High-performance liquid chromatography of selenium compounds utilizing perfluorinated carboxylic acid ion-pairing agents and inductively coupled plasma and electrospray ionization mass spectrometric detection

Kotrebai, Mihály; Tyson, Julian F.; Block, Eric; Uden, Peter C.

doi:10.1016/s0021-9673(99)01060-2

Cited by 137 publications

(108 citation statements)

References 41 publications

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“…The good separation of different selenium species is the prerequisite for the purpose of speciation analysis. AE HPLC 15,28 and RP HPLC 29,30 are the popular separation technique for selenium species. In this work, we test the application of the different HPLC modes in selenium separation.…”

Section: Resultsmentioning

confidence: 99%

Quantitative Speciation of Selenium in Human Blood Serum and Urine with AE- RP- and AF-HPLC-ICP/MS

Jeong¹,

Lee²,

Pak

2013

Bulletin of the Korean Chemical Society

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Various separation modes in HPLC, such as anion exchange (AE), reversed-phase (RP), and affinity (AF) chromatography were examined for the separation of selenium species in human blood serum and urine. While RP-and AE-HPLC were mainly used for the separation of small molecular selenium species, double column AF-HPLC achieved the separation of selenoproteins in blood serum efficiently. Further, the effluent of AF-HPLC was enzymatically hydrolyzed and then analyzed with RP HPLC for selenoamino acid study. The versatility of the hybrid technique makes the in-depth study of selenium species possible. For quantification, post column isotope dilution (ID) with 78Se spike was performed. ORC ICP/MS (octapole reaction cell inductively coupled plasma/mass spectrometry) was used with 4 mL min −1 Hydrogen as reaction gas. In urine sample, inorganic selenium and SeCys were identified. In blood serum, selenoproteins GPx, SelP and SeAlb were detected and quantified. The concentration for GPx, SelP and SeAlb was 22.8 ± 3.4 ng g Enzymatic hydrolysis of each selenium proteins revealed that SeCys is the major amino acid for all three proteins and SeMet is contained in SeAlb only.

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Section: Resultsmentioning

confidence: 99%

Quantitative Speciation of Selenium in Human Blood Serum and Urine with AE- RP- and AF-HPLC-ICP/MS

Jeong¹,

Lee²,

Pak

2013

Bulletin of the Korean Chemical Society

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“…Five selenium species and several unknown peaks were observed in selenium-enriched garlic, onion, and broccoli [22,23]. Several seleno-analogues of sulfur-containing amino acids and their derivatives have been identified in terrestrial plants, especially in so-called "selenium accumulator plants" [24][25][26]. Among the six organoselenium compounds detected in seagull eggs the major compounds were selenocysteine and selenocystamine [27].…”

Section: Species Of Interestmentioning

confidence: 99%

“…Ion-exchange and reversed-phase HPLC have been mainly applied to the separation of Se(IV), Se(VI), selenomethionine, and selenocysteine (for an exhaustive list of references see [17,18,55]). In recent studies a large number of synthetic organoselenium compounds were used [24][25][26].…”

Section: Hplc With Selenium-specific Detectionmentioning

confidence: 99%

“…Fan et al [29] synthesized the selenium metabolite compounds Se-methylselenomethionine, Semethylselenocysteine, and dimethylselenonium propionate, and employed a combination of 2D multinuclear NMR, electrospray MS and GC-MS methods to identify Se-compounds in synthetic preparations [29]. Uden et al synthesized a number of selenoamino acids and tried to match their retention times with signals produced by extracts of yeast supplements [18,23,24,25,26]. The success of these approaches so far has been moderate.…”

Section: Identification Of Selenium Speciesmentioning

confidence: 99%

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Species-selective determination of selenium compounds in biological materials (Technical Report)

Łobiński

Edmonds

Suzuki

et al. 2000

Pure and Applied Chemistry

157

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Republication or reproduction of this report or its storage and/or dissemination by electronic means is permitted without the need for formal IUPAC permission on condition that an acknowledgmentMembership of the Commission during the preparation of this report (1997)(1998)(1999) Species-selective determination of selenium compounds in biological materials (Technical Report)Abstract: There is substantial evidence of the complexity of selenium speciation in living organisms and of the importance of the selective determination of the particular species of this element in order to understand its metabolism and biological significance in clinical chemistry, biology, toxicology, and nutrition. The state-of-the-art of analytical techniques available for this purpose is critically evaluated with particular emphasis on the element-selective detection and identification of the detected selenium compounds. Whereas there are a number of techniques available that are able to detect various selenium species in living organisms selectively, few techniques exist that are able to identify and to characterize the species detected.

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“…As a complementary technique to LC-ICP-MS, electrospray ionization (ESI) or atmospheric pressure chemical ionization (APCI)-mass spectrometry was used for the identification of unknown Se-containing metabolites in biological samples. [10][11][12][13][14][15] Since ESI is a softer technique than ICP, it can provide molecular information. 16 Hence, ICP-MS and ESI/APCI-MS are occasionally called inorganic and organic mass spectrometry, respectively.…”

Section: Introductionmentioning

confidence: 99%

Complementary Use of LC-ICP-MS and LC-ESI-Q-TOF-MS for Selenium Speciation

2015

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Experimental ReagentsSodium selenate and ammonium acetate were purchased from Wako (Osaka, Japan).L-Selenomethionine (SeMet) and trimethylselenonium iodide (TMSe) were purchased from Sigma (St. Louis, MO) and Tri Chemical Laboratories, Inc. 2015 © The Japan Society for Analytical Chemistry † To whom correspondence should be addressed. E-mail: ogra@chiba-u.jp Complementary Use of LC-ICP-MS and LC-ESI-Q-TOF-MS for Selenium SpeciationYasumi ANAN,* Genki NAKAJIMA,* and Yasumitsu OGRA* , ** † We demonstrated the complementary use of inductively coupled plasma-mass spectrometry (ICP-MS) and electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-Q-TOF-MS) for the analysis of Se-containing compounds, such as selenate, selenomethionine (SeMet), and trimethylselenonium ion (TMSe), found in biological samples. The sensitivity of ESI-Q-TOF-MS for Se-containing compounds was strongly dependent on the chemical species. ICP-MS exhibited higher sensitivity than ESI-Q-TOF-MS, and had no species dependency. On the other hand, ESI-Q-TOF-MS enabled easy and robust identification of Se-containing compounds.

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High-performance liquid chromatography of selenium compounds utilizing perfluorinated carboxylic acid ion-pairing agents and inductively coupled plasma and electrospray ionization mass spectrometric detection

Cited by 137 publications

References 41 publications

Quantitative Speciation of Selenium in Human Blood Serum and Urine with AE- RP- and AF-HPLC-ICP/MS

Quantitative Speciation of Selenium in Human Blood Serum and Urine with AE- RP- and AF-HPLC-ICP/MS

Species-selective determination of selenium compounds in biological materials (Technical Report)

Complementary Use of LC-ICP-MS and LC-ESI-Q-TOF-MS for Selenium Speciation

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