Complementary Use of LC-ICP-MS and LC-ESI-Q-TOF-MS for Selenium Speciation

Abstract

Experimental ReagentsSodium selenate and ammonium acetate were purchased from Wako (Osaka, Japan).L-Selenomethionine (SeMet) and trimethylselenonium iodide (TMSe) were purchased from Sigma (St. Louis, MO) and Tri Chemical Laboratories, Inc. 2015 © The Japan Society for Analytical Chemistry † To whom correspondence should be addressed. E-mail: ogra@chiba-u.jp Complementary Use of LC-ICP-MS and LC-ESI-Q-TOF-MS for Selenium SpeciationYasumi ANAN,* Genki NAKAJIMA,* and Yasumitsu OGRA* , ** † We demonstrated the complementary use of inductively coupled plasma-mass spectrometry (ICP-MS) and electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-Q-TOF-MS) for the analysis of Se-containing compounds, such as selenate, selenomethionine (SeMet), and trimethylselenonium ion (TMSe), found in biological samples. The sensitivity of ESI-Q-TOF-MS for Se-containing compounds was strongly dependent on the chemical species. ICP-MS exhibited higher sensitivity than ESI-Q-TOF-MS, and had no species dependency. On the other hand, ESI-Q-TOF-MS enabled easy and robust identification of Se-containing compounds.

Inductively Coupled Plasma-Mass Spectrometry

“…Anan et al reported on "Complementary Use of LC-ICP-MS and LC-ESI-Q-TOF-MS for Selenium Speciation", describing the analytical performance of these techniques for Se-containing compounds, such as selenate, selenomethionine (SeMet), and trimethylselenonium ion (TMSe). 9 This study indicated that the sensitivity of LC-ICP-MS was higher than that of LC-ESI-Q-TOF-MS. Iwai et al reported on "Arsenic Speciation and Cadmium Determination in Tobacco Leaves, Ash and Smoke". 10 Tobacco leaves, ash, and smoke samples were prepared and the concentrations of As and Cd were determined after microwave-assisted digestion.…”

Atomic Spectrometry Update: review of advances in elemental speciation

“…A technical note which reports the advantages and disadvantages of ICP-MS and ESI-MS for Se speciation in biological samples has been presented this year. 169 The article includes a summary table with information on LOD values and sensitivity provided by ICP-MS and ESI-QTOF-MS for the determination of Se VI , SeMet and TMSe. Based on their own experimental data, the authors underline that the most effective use of ESI-MS is for species identication in conjunction with ICP-MS for species quantitation.…”

Simultaneous Species Analysis of Arsenic, Selenium, Bromine, and Iodine in Bottled Drinking Water and Fruit Juice by High-Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry

“…17 However, high (>levels of iodate 600 mg day -1 ) have been shown to cause ocular toxicity by damaging the retina. 18 At present, the studies of element species in water and food samples were mostly focused on the analysis of single element, [19][20][21][22][23][24] and there are few reports on simultaneous analysis of multielement species. The simultaneous analysis and determination of multi-element species can significantly improve the analysis efficiency and save testing costs.…”