High Performance Liquid Chromatography of Natural Products

Kingston, David G. I.

doi:10.1021/np50003a001

Cited by 40 publications

(12 citation statements)

References 43 publications

(46 reference statements)

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“…Though numerous phenolic plant constituents have successfully been separated by HPLC [6][7][8], few reports on isoflavones had appeared [9][10][11] at the beginning of our studies. Most of these failed to describe simultaneous separation of both aglycones and glucosides.…”

Section: Hpl C Separation Of Isoflavonesmentioning

confidence: 99%

High Performance Liquid Chromatographic Separation of Isoflavones and Structural Elucidation of Isoflavone 7-O-glucoside 6″-malonates from Cicer arietinum

1983

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Section: Hpl C Separation Of Isoflavonesmentioning

confidence: 99%

High Performance Liquid Chromatographic Separation of Isoflavones and Structural Elucidation of Isoflavone 7-O-glucoside 6″-malonates from Cicer arietinum

1983

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“…For the separation and quantification of natural products in complex mixtures, high performance liquid chromatography (HPLC) is the method of choice. The reversed-phase technique (RP-HPLC) is the most useful in the flavonoid field [1][2][3]. Many papers show the analysis of more or less purified flavonoid mixtures, for qualitative or quantitative determinations in plants [4][5][6][7], food [8] and drugs [9][10][11], and for chemotaxonomic studies [12,13].…”

Section: Introductionmentioning

confidence: 99%

HPLC Separation and Quantitative Determination of Biflavones in Leaves fromGinkgo biloba

Briançon-Scheid¹,

Lobstein-Guth²,

Anton³

1983

Planta Med

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An HPLC method for separation and quantitative determination of biflavones in crude leaf extracts from GINKGO BILOBA L. is described. A system using a Lichrosorb(R)-Diol column and the ternary elution system: hexane-chloroform-tetrahydrofuran, was suitable for separation of sciadopitysin, ginkgetin, isoginkgetin and bilobetin. The biflavones were detected at 330 nm; their calibration curves were established and their response factors were calculated using acacetin as internal standard. This method was used for the quantitative determination of the biflavones in crude leaf extracts; the seasonal variations of the biflavone content were studied and showed a higher amount in autumn leaves.

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“…Reviews on equipment and instrumentation are published periodically in journals such as Analytical Chemistryfor example, by LaCourse [20]. An excellent early comprehensive review on the analysis of natural products was provided by Kingston in 1979 [21]. Since then, there have been numerous developments but the same basic conditions predominate, i.e., octadecylsilyl columns and methanol-water or acetonitrile-water as eluent.…”

Section: Column Chromatographic Techniquesmentioning

confidence: 99%

Natural Product Analysis over the Last Decades

Marston

Hostettmann²

2009

Planta Med

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Progress in natural product chemistry has always been strongly linked to innovations in analytical technology. The characterisation of metabolites in complex mixtures requires sophisticated techniques, which should provide good sensitivity and selectivity as well as structural information on the constituents of interest. This review outlines the most important chromatographic and spectral techniques which have been introduced in the field of natural products. Although there has been a very rapid evolution of methods over the last 50 years, the introduction of highthroughput screening programmes require even more efficient and sensitive methodologies which yield adequate on-line information for metabolite structure determinatio

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High Performance Liquid Chromatography of Natural Products

Cited by 40 publications

References 43 publications

High Performance Liquid Chromatographic Separation of Isoflavones and Structural Elucidation of Isoflavone 7-O-glucoside 6″-malonates from Cicer arietinum

High Performance Liquid Chromatographic Separation of Isoflavones and Structural Elucidation of Isoflavone 7-O-glucoside 6″-malonates from Cicer arietinum

HPLC Separation and Quantitative Determination of Biflavones in Leaves fromGinkgo biloba

Natural Product Analysis over the Last Decades

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