High‐performance liquid chromatography/nano‐electrospray ionization tandem mass spectrometry, two‐dimensional difference in‐gel electrophoresis and gene microarray identification of lymphatic metastasis‐associated biomarkers

Liu, Shuqing; Sun, Ming‐Zhong; Tang, Jianwu; Wang, Zhiqiang; Sun, Chengrong; Greenaway, Frederick T.

doi:10.1002/rcm.3725

Cited by 51 publications

(38 citation statements)

References 45 publications

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“…Previous proteomics work from our group, which used two-dimensional difference in gel electrophoresis (2-D DIGE) coupled high-performance liquid chromatography/HPLC-electro-spray ionisation mass spectrometry (HPLC-nESI-MS/MS) analysis, indicated that 113 proteins were differentially expressed between the two cell lines. The expression levels of annexinA7, Uchl3 and ER protein 29 were doubly validated by Western blotting analysis [2,8]. Our proteomic results of Uchl3 and ERp29 are consistent with their role in the progression of breast cancer [8,9] and protein function [10].…”

Section: Progress In Cellular Proteomic Research On Hepatocarcinomasupporting

confidence: 84%

“…Therefore, annexin A7 is functionally involved in the tumour malignancy progression and is potentially associated with LNM [8]. Seventeen out of the identifi ed proteins, including Fbp2, Glud1, Crk, Ndrg1, Tkt, Anxa5, Krt2-7, Krt2-8 and Lactb2, were found to be differentially expressed, in accordance with their mRNA levels, which might be critical protein candidates associated with the LNM of hepatocarcinoma [2].…”

Section: Progress In Cellular Proteomic Research On Hepatocarcinomamentioning

confidence: 83%

“…It is a complicated dynamic network system involving multiple factors [2]. The lymphatic system is involved in metastases of a variety of cancers with increased incidences of lymph node metastasis (LNM).…”

Section: Introductionmentioning

confidence: 99%

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Proteomic research progress in lymphatic metastases of cancers

et al. 2012

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Lymph node metastasis (LNM) is recognised as an important factor involved in malignant tumour progression by interfering with a favourable prognosis. It is involved in a variety of cancers. Proteins are believed to play important roles in the LNM of cancers. The rapid achievements of state-of-the-art proteomic techniques have emerged as the key technologies successfully applied to identify markers for cancers at high-throughput level by providing novel targets and creating possible therapeutic interventions in cancer research. This review summarises recent progress in proteomic research in hepatocarcinoma, gastric cancer, oesophageal cancer, colorectal cancer, breast cancer, lung cancer and nasopharyngeal cancer. Actin, heat-shock proteins (HSPs), annexins, cytokeratin 10 (CK10), CK19, protein gene product 9.5 (PGP9.5) and protein disulfide isomerase (PDI) are the most common proteins in lymphatic metastases of cancers revealed by proteomic and protein functional studies. Other protein candidates specifically associated with LNMs of certain cancers are also summarised and discussed.

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Section: Progress In Cellular Proteomic Research On Hepatocarcinomasupporting

confidence: 84%

Section: Progress In Cellular Proteomic Research On Hepatocarcinomamentioning

confidence: 83%

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Proteomic research progress in lymphatic metastases of cancers

et al. 2012

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“…Alterations in the activity of human TKT have been reported to cause and/or accompany different pathological disorders, including the Wernicke-Korsakoff syndrome, Alzheimer disease, or diabetes (14 -16). Furthermore, TKT was suggested to be a critical determinant for impaired hippocampal neurogenesis, lymphatic metastasis of hepatocarcinoma, fibromyalgia, tumor cell growth, resistance of colon cancer toward 5-fluoruracil, and female fertility (17)(18)(19)(20)(21).…”

Section: Reactions 1 Andmentioning

confidence: 99%

The Crystal Structure of Human Transketolase and New Insights into Its Mode of Action

Mitschke

Parthier

Schröder-Tittmann

et al. 2010

Journal of Biological Chemistry

171

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The crystal structure of human transketolase (TKT), a thiamine diphosphate (ThDP) and Ca 2؉ -dependent enzyme that catalyzes the interketol transfer between ketoses and aldoses as part of the pentose phosphate pathway, has been determined to 1.75 Å resolution. The recombinantly produced protein crystallized in space group C2 containing one monomer in the asymmetric unit. Two monomers form the homodimeric biological assembly with two identical active sites at the dimer interface. Transketolase (TKT 3 ; EC 2.2.1.1) is a ubiquitous enzyme in cellular carbon metabolism and requires thiamine diphosphate (ThDP), the biologically active derivative of vitamin B1, and Ca 2ϩ ions as cofactors for enzymatic activity (1, 2). TKT catalyzes the reversible transfer of two-carbon (1,2-dihydroxyethyl) units from ketose phosphates to the C1 position of aldose phosphates and thus provides, together with the Schiff base-forming transaldolase, a reversible link between glycolysis and the pentose phosphate pathway. This shunt permits cells a flexible adaptation to different metabolic needs as the pentose phosphate pathway supplies intermediates for other metabolic pathways; generates precursors for biosynthesis of nucleotides, aromatic amino acids, and vitamins; and further produces NADPH for sustaining the glutathione level and for reductive biosynthetic pathways of, for example, cholesterol and fatty acids.TKT acts on different ketose phosphate (donor) and aldose phosphate (acceptor) substrates of variable carbon chain length (3-7 carbons) in two major, essentially reversible reactions. REACTIONS 1 AND 2A simplified reaction scheme for the TKT-catalyzed conversion of substrates X5P and R5P into products S7P and G3P is shown in Fig. 1. The reaction cycle can be subdivided into a donor half-reaction (donor ligation and cleavage) and an acceptor half-reaction (acceptor ligation and product liberation) (3).After formation of the reactive ylide form of ThDP, the C2 carbanion of ThDP attacks the carbonyl of donor X5P in a nucleophilic manner to yield the covalent donor-ThDP adduct X5P-ThDP (step 1). Ionization of C3-OH and cleavage of the scissile C2-C3 bond of X5P-ThDP results in the formation of product G3P and of the 1,2-dihydroxylethyl-ThDP (DHEThDP) carbanion/enamine intermediate (step 2). This intermediate may then react with either G3P (reverse reaction of step 2) or R5P in competing equilibria. In the latter case, C2␣ of DHE-ThDP ligates to C1 of R5P (in the acyclic form), yielding * This work was supported by a grant from the "Fonds der Chemischen Industrie" (stipend to S. L.) and the Deutsche Forschungsgemeinschaft-funded Gö ttingen Graduate School for Neurosciences and Molecular Biosciences (to K. T.

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“…integrins [86](Unpublished). This was associated with increased cell adhesion to fibronectin, collagen, and matrigel extracellular matrices.…”

Section: Lung Cancermentioning

confidence: 99%

The role of a7 nicotine acetylcholine receptors in lung injury and repair.

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High‐performance liquid chromatography/nano‐electrospray ionization tandem mass spectrometry, two‐dimensional difference in‐gel electrophoresis and gene microarray identification of lymphatic metastasis‐associated biomarkers

Cited by 51 publications

References 45 publications

Proteomic research progress in lymphatic metastases of cancers

Proteomic research progress in lymphatic metastases of cancers

The Crystal Structure of Human Transketolase and New Insights into Its Mode of Action

The role of a7 nicotine acetylcholine receptors in lung injury and repair.

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