High-performance liquid chromatography method development and validation for the quantification of mangiferin in Coffea arabica leaves

Abstract

Introduction and Aim: Mangiferin is accompanied by the therapeutic potential for various human ailments. The present study is aimed to develop a validated HPLC method for the quantification of mangiferin in coffee (Coffea arabica) leaves.
 
 Materials and Methods: The ­­high-performance liquid chromatography (HPLC) was carried out on Agilent Technologies 1260 LC Infinity Series liquid chromatography system (Agilent, USA) using an isocratic mobile phase of a mixture of methanol and 1% orthophosphoric acid v/v (70:30) at a flow rate 1mL min-1 for 14 min with the controlled temperature at 25°C. The mangiferin content was detected by a UV diode array detector at 258 nm.
 
 Results: The linearity of the HPLC system was established in the range of 10-100 ppm mL-1 with a regression coefficient (R2) of 0.99670. The accuracy of the method was assessed by a study performed using 3 different levels, with a 99% average recovery. The limit of detection(LOD) and limit of quantification (LOQ) were 1.3 and 3.9 ppm mL-1. Intraday and interday precision analysis showed the relative standard deviation 0.012342 separately. The content of mangiferin in the coffee leaves was 94 mg/gram (w/w).
 
 Conclusion: All necessary factors, such as linearity, accuracy, LOD, LOQ, precision, and % relative standard deviation(RSD), were inside the appropriate limits. The HPLC was validated using all necessary variables with a successful estimation of mangiferin.

Synthesis of Substituted Arylidene Hydrazinyl Trifluoromethyl Thiazole Derivatives and their Antibacterial Studies using different Genes Expression

“…1H singlet in the range 7.11-7.87 ppm is ascribed to thiazole proton at C-5 position for each compound. The structures of 3 a-m were further verified by 13 C NMR spectroscopy where the azomethine linkages were identified in the range 133.5-143.3 ppm. Furthermore, 13 C NMR allowed us to identify the substitution pattern in the thiazole: the C2 carbon resonates at 169.3-171.4 ppm, the C4 in the range Table 1.…”

“…Total RNA from bacterial strains was extracted and purified using an RNA extraction kit (Promega Corporation USA) following the manufacturer's instructions, and the RNA concentration and purity were evaluated using the Nano Drop device. [13] The RNA was reverse transcribed into cDNA, and the contents of the tubes were thoroughly mixed with a mixer before being placed in a qRT-PCR device, the replication steps were performed to investigate the amount of gene expression (recAE, FLU) in E. coli and (recAE, mexB) in K. pneumoniae against thirteen treatments (3 a-m). The qRT-PCR apparatus was set up, and the Cycle threshold (Ct) value was determined using the Livak equation.…”

“…The gene expression of the FLU genes was measured using qRT-PCR technique [13] for E. coli. The extent of gene expression and the Cycle Threshold (Ct) was determined.…”