High-performance liquid chromatography–mass spectrometric analysis of furosemide in plasma and its use in pharmacokinetic studies

Analytical issues in HPLC/MS/MS simultaneous assay of furosemide, spironolactone and canrenone in human plasma samples

“…Although UV spectrometric detection has been used in the early nineties [2,3], it seems that fluorescence detection was preferred for furosemide together with the ion pair separation mechanism [4,5]. Mass spectrometry (MS) and tandem mass spectrometry (MS/MS) have also been used to determine furosemide in biological samples, more often by applying negative ion monitoring and atmospheric pressure chemical ionization (APCI) [6,7]. Liquid-liquid extraction (LLE) and solid phase extraction (SPE) are generally cited as sample preparation methods for the assay of furosemide in biological fluids [8].…”

Unlocking the Chemotherapeutic Potential of β‐Aminovinyl Ketones and Related Compounds

“…Recently, HPLC-MS has been used as an alternative to HPLC in many clinical investigations such as metabolic and pharmacokinetics studies. [37,38] For carrying out biological studies on the anticonvulsant activity of enaminones in animals, a sensitive and selective HPLC-MS method was developed for the determination of selected aminovinyl ketones in rat serum [39] to discern the possible role of metabolites as the active/co-active entities of enaminone esters. The most active compound was ethyl-4-[(4-chlorophenyl)amino]-6methyl-2-oxocyclohex-3-ene-1-carboxylate (13), which displayed an ED 50 value of 16.7 mg kg À1 .…”

Recent developments in analytical determination of furosemide

“…The high specificity and sensitivity with marked short time of analysis are prominent advantages of LC-MS. Abdel-Hamid presents a new investigation to elucidate the potential of LC-MS in the determination of FUR, using diclofenac as IS, in plasma with one-step extraction procedure [98]. Both compounds were extracted from human plasma with ethyl acetate at pH 1 and were chromatographed using Shim-Pack GLC-CN column and a mobile phase consisting of acetonitrile and 20 mM ammonium acetate buffer solution pH 7, 4:1 (v:v) at a flow-rate 1 mL min −1 .…”