High-performance liquid chromatography (HPLC)-size exclusion chromatography (SEC) for qualitative detection of humic substances and dissolved organic matter in mineral soils and peats in Lithuania

Jokubauskaitė, Ieva; Amalevičiūtė, Kristina; Lepane, Viia; Šlepetienė, Alvyra; Šlepetys, J.; Liaudanskienė, Inga; Karčauskienė, Danutė; Booth, Colin A.

doi:10.1080/03067319.2015.1048435

Cited by 21 publications

(12 citation statements)

References 24 publications

(26 reference statements)

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“…2). Among humic substances, the large-molecule fraction dominates [28][29][30]; however, in the studied water the molecular sizes were significantly smaller than found in other studies. Additionally, it must be noticed that among dissolved organic substances, the biodegradable fraction (substances of a low molecular mass) [31] made up 14.9-31.3% of TOC.…”

Section: Resultscontrasting

confidence: 67%

Effectiveness of humic substance removal during the coagulation process

2019

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This study concerns the utility of the coagulation process in removing humic substances and its dependence on the properties of these substances and their concentrations. The coagulation process was performed on model solutions of natural humic acids. Polyaluminum chloride (PAX XL3) and aluminum sulfate were used for this study, which allowed for a comparison of the effectiveness of pre-hydrolyzed and hydrolyzing coagulants. The coagulant dosages were determined as gram aluminum per gram carbon, thanks to which it was possible to compare the process effectiveness for different initial organic carbon concentrations, whose values were in the range of 5.51-21.23 gC/m 3. The obtained values of reductions in organic carbon concentrations point to a significant process effectiveness (37.2-59.4% and 20.0-41.5% for pre-hydrolyzed and hydrolyzing coagulants, respectively), which increased with increasing molecular mass of the humic substances present in water. These results are analogous to those found for coagulation of surface waters and point to a greater effectiveness of pre-hydrolyzed coagulant in removing humic substances (at the same coagulant dosage). The effectiveness in removing organic substances increased with coagulant dosage and the initial total organic carbon concentration. The coagulation process most effectively removed aromatic substances absorbing UV light. The content of substances absorbing UV light in raw and post-coagulation water was proportional to the dissolved organic carbon content.

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Section: Resultscontrasting

confidence: 67%

Effectiveness of humic substance removal during the coagulation process

2019

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“…() examined the molecular size of DOM extracted from organic horizons of a Podzol using HPSEC and showed that the apparent molecular weight of tested DOM generally ranged between 470 and 16,890 Da with a number‐average molecular weight of 1791 Da and a weight‐average molecular weight (M w ) of 2468 Da. Similarly, a M w of 2439 Da was obtained for the DOM from mineral soils, but a higher M w of 3436 Da for peat samples ( Jokubauskaite et al., ). Chorover and Amistadia () showed that most of the DOM from forest floor leachates was smaller than 10 kDa and the M w was between 5–6 kDa.…”

Section: Tracing the Ambivalence Of The Multilayer Modelmentioning

confidence: 76%

“…The apparent molecular weight distribution of DOM from wetland soils was tested by Rouwane et al (2018) using high performance size exclusion chromatography (HPSEC), and they reported that the prevailing apparent molecular weight ranges from 0.5 to 6 kDa (> 80% according to chromatogram area). (Jokubauskaite et al, 2015). Chorover and Amistadia (2001) showed that most of the DOM from forest floor leachates was smaller than 10 kDa and the M w was between 5-6 kDa.…”

Section: Molecular Size Of Soil Dissolved Organic Matter and Potentiamentioning

confidence: 99%

The multilayer model of soil mineral–organic interfaces—a review

Gao

Mikutta

Jansen

et al. 2019

J. Plant Nutr. Soil Sci.

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Association of organic matter (OM) with minerals is an important pathway in the formation of stable OM in soil. While the importance of mineral-organic associations (MOA) in regulating soil carbon cycling has been rigorously demonstrated by empirical evidence, knowledge about the molecular-scale arrangement of OM at mineral surfaces is still lacking. Such knowledge is urgently needed to disentangle the mechanisms of long-term storage of soil OM. Based on indirect observations regarding the formation, composition, and structure of MOA, a conceptual multilayer model was proposed by Kleber et al. in 2007 to foster debate and help elucidating the structure and reactivity of MOA. According to this model, the associated OM at mineral surfaces is discrete and self-organized into a multilayer structure. In this review, we aim to collect and evaluate existing studies that used this model to explain biogeochemical processes at mineral-organic interfaces, and based on this, assess the applicability of the model. The multilayer model has seen extensive adoption within soil science and related fields. In general, existing studies either support the concept of a patchy distribution of adsorbed OM on mineral surfaces or advocate that OM can be coprecipitated with nanosized poorly crystalline minerals or hydrolysable metals. However, the evidence for the patchy distribution of adsorbed OM cannot support the multilayer model on its own. There is little consensus about the role of N-rich OM in forming the contact zone according to the multilayer model but surface conditioning by different classes of organic compounds appears to be an essential factor for the overall adsorption of OM. Nevertheless, large uncertainty still remains with respect to multilayer-like organization of MOA. By taking advantage of recent developments in surface analytical sciences and computational chemistry, a rigid experimental testing of the multilayer model at the molecular level is still required and awaits to be integrated into improved concepts of MOA formation and OM stabilization.

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“…Studies of the effect of liming on the individual groups and fractions of soil organic matter remain among the priority tasks for theory and application of liming. Despite the complexity of this analysis, recently, there has been an increase in the number of similar works (Takahashi et al, 2006;Litvinovich, Pavlova, 2007;Zavyalova, 2010;Liaudanskienė et al, 2011;Jokubauskaite et al, 2015). In the process of liming, simultaneous changes of soil acidity and content of mobile forms of elements that primarily bound humic substances in soil (Al, Fe and Ca) take place (Takahashi et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Empirical models of transformations of humic acids and humin in Umbric Albeluvisol Abruptic as influenced by liming

Litvinovich

Pavlova

Lavrishchev

et al. 2017

Zemdirbyste-Agriculture

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The research studied the changes in total carbon (C) content-carbon of humic acids (HA), and non-hydrolysable residue-humin (NR) in Umbric Albeluvisol Abruptic (umABap) under application of a wide range of doses (0.1-2.5 hydrolytic acidity (Hy), meq per 100 g of soil) of lime in the controlled conditions of a pot experiment. The trend of decreasing of total humus content in the soil was revealed only under application of the highest dose (2.5 Hy) of lime. A gradual decline in the proportion of humin and increase in the proportion of humic acids correspondingly to the doses of lime was observed. The correlation coefficient between humin and ƩHA was r = −0.49. Moderately negative correlation between the content of humin and humic acid 1 (HA1) "free" and bound with sesquioxides was found: r = −0.37; between humin and humic acid 2 (HA2), bound to calcium: r = −0.75. HA1 and HA2 fractions were not significantly correlated. The increase in the humus content of humic acid 3 (HA3) that is tightly bound with clay minerals and stable forms of sesquioxides was found only starting from the dose of lime corresponding to 0.9 Hy. At a dose of lime, equal to 2.5 Hy its content in humus exceeded the content of HA2. The hypothesis of the research suggesting that the source of replenishment of the portion of humic acids might be the organic substances of humin was proved. The empirical models of transformation of humic acid and humin as influenced by increasing doses of lime and in the process of its dissolution were developed. Optical densities of humic acids were presented.

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High-performance liquid chromatography (HPLC)-size exclusion chromatography (SEC) for qualitative detection of humic substances and dissolved organic matter in mineral soils and peats in Lithuania

Cited by 21 publications

References 24 publications

Effectiveness of humic substance removal during the coagulation process

Effectiveness of humic substance removal during the coagulation process

The multilayer model of soil mineral–organic interfaces—a review

Empirical models of transformations of humic acids and humin in Umbric Albeluvisol Abruptic as influenced by liming

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