High performance liquid chromatography equipped with a cathodic detector and column-switching device as a high-throughput method for a phosphatase assay with p-nitrophenyl phosphate

Yamauchi, Yuji; Ido, Megumi; Maeda, Hatsuo

doi:10.1016/j.chroma.2005.01.071

Cited by 37 publications

(9 citation statements)

References 17 publications

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“…Traditional assays for ACP are based on colorimetric determination of hydrolysis products of its substrates, which varied from p -nitrophenylphosphate and phosphotyrosine to 2,6-dichloro-4-acetylphenyl phosphate. − Although several assays based on potentiometric and chromatographic approaches were established to detect ACP level, − most efforts have been devoted to fluorometric assay because of its high sensitivity, convenience, and accessible instrument requirement. To date, only a few fluorescent assays for ACP have been proposed, and most of them mainly adopted organic dyes or fluorescent polymers as the fluorogenic indicators.…”

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confidence: 99%

Luminescent Aggregated Copper Nanoclusters Nanoswitch Controlled by Hydrophobic Interaction for Real-Time Monitoring of Acid Phosphatase Activity

et al. 2016

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A reversible luminescence nanoswitch through competitive hydrophobic interaction among copper nanoclusters, p-nitrophenol and α-cyclodextrin is established, and a reliable real-time luminescent assay for acid phosphatase (ACP) activity is developed on the basis of this luminescence nanoswitch. Stable and intensely luminescent copper nanoclusters (CuNCs) were synthesized via a green one-pot approach. The hydrophobic nature of CuNCs aggregate surface is identified, and further used to drive the adsorption of p-nitrophenol on the surface of CuNCs aggregate due to their hydrophobic interaction. This close contact switches off the luminescence of CuNCs aggregate through static quenching mechanism. However, the introduction of α-cyclodextrin switches on the luminescence since stronger host-guest interaction between α-cyclodextrin and p-nitrophenol causes the removal of p-nitrophenol from the surface of CuNCs. This nanoswitch in response to external stimulus p-nitrophenol or α-cyclodextrin can be run in a reversible way. Luminescence quenching by p-nitrophenol is further utilized to develop ACP assay using p-nitrophenyl phosphate ester as the substrate. Quantitative measurement of ACP level with a low detection limit of 1.3 U/L was achieved based on this specific detection strategy. This work reports a luminescence nanoswitch mediated by hydrophobic interaction, and provides a sensitive detection method for ACP level which is capable for practical detection in human serum and seminal plasma.

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confidence: 99%

Luminescent Aggregated Copper Nanoclusters Nanoswitch Controlled by Hydrophobic Interaction for Real-Time Monitoring of Acid Phosphatase Activity

et al. 2016

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“…Various methods for detecting ACP activity have been proposed to assess the functional capacity of the prostate, such as high-performance liquid chromatography (HPLC) [6], surface-enhanced Raman spectroscopy (SERS) [7], immunoassay [8] and voltammetry [9]. These approaches have achieved sensitive and accurate detection of ACP, but are not feasible for on-site and rapid assay.…”

Section: Introductionmentioning

confidence: 99%

A dual-signal fluorescent probe for detection of acid phosphatase

Wang

et al. 2021

Anal Bioanal Chem

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Acid phosphatase has become a significant indicator of prognostic and medical diagnosis, and its dysfunction may lead to a series of diseases. A novel dual-signal fluorescence method for acid phosphatase detection based on europium polymer (europiumpyridine dicarboxylicacid-adenine) and pyridoxal phosphate (PLP) was proposed. PLP coordinated with europium polymer via Eu 3+ and P-O bonds, and the fluorescence of europium polymer was quenched due to the photoinduced electron transfer (PET) effect between aldehyde and europium polymer. Upon addition of acid phosphatase, the PLP was transformed to phosphate (Pi) and pyridoxal (PL). The PL was released from the surface of europium polymer, and the blue emission was enhanced due to the formation of internal hemiacetal, while the fluorescence of europium polymer recovered. The blue (PL) and red emission (Eu 3+ ) were positively correlated with acid phosphatase activity; thus the sensitive assay of acid phosphatase was effectively achieved. The two signals were applied to determine the acid phosphatase with limits of detection (LOD) of 0.04 mU/mL and 0.38 mU/mL, and the linear ranges were 0.13-5.00 mU/mL and 1.25-20.00 mU/mL, respectively. The probe can be used to trace the acid phosphatase in biological systems and holds promise for use in clinical diagnosis and early prevention.

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“…In addition, some subtypes of ACPs, including phosphatidic acid phosphatase type 2C 4 and tartrate-resistant acid phosphatase (TRAP), have been recognized as promising drug targets. , Hence, the development of convenient, sensitive, and selective methods toward ACP is of great importance in both diagnostics and drug screening. In recent years, several ACP monitoring strategies have been explored, including spectrophotometry such as fluorometric and colorimetric methods, , high-performance liquid chromatography, surface acoustic wave sensors, and electrochemical methods . Although the above methods can quantitatively detect ACP with efficient sensitivity, most of them show drawbacks such as tedious sample preparation, complicated instrumental setup, costly signal labels, and limited application range.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Colorimetric Detection of Acid Phosphatase and Screening of Its Inhibitors Based on Biomimetic Oxidase Activity of MnO₂ Nanosheets

Wang

Weng

et al. 2020

ACS Biomater. Sci. Eng.

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In this research, we attempted to develop a sensitive colorimetric sensing strategy for the detection of acid phosphatase (ACP) based on MnO2 nanosheets and explored its applications in screening and evaluating inhibitors of ACP. The MnO2 nanosheets exhibit intrinsic biomimetic oxidase activity, which can catalyze the oxidation of the colorless 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonate) diammonium salt (ABTS) into green oxidized ABTS (oxABTS). Upon the introduction of ACP, l-ascorbic acid-2-phosphate can be dephosphorylated to ascorbic acid, which arouses the disintegration of MnO2 nanosheets into Mn2+ ions. This disintegration weakens the enzyme mimicking activity of the MnO2 nanosheets, leading to the impediment of the oxidation of ABTS. Conversely, in the absence of ACP, the ABTS is rapidly oxidized by MnO2, leading to a significant colorimetric signal change. The absorbance difference at 420 nm displayed a linear relationship with the concentration of ACP ranging from 0.075 to 0.45 mU·mL–1, generating a detection limit of 0.046 mU·mL–1. In the inhibition assays, this sensing platform provided simple detection for parathion-methyl (PM), a representative inhibitor of ACP. The facile evaluation of the inhibitory effect of PM, including its IC50 toward ACP, was also realized.

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High performance liquid chromatography equipped with a cathodic detector and column-switching device as a high-throughput method for a phosphatase assay with p-nitrophenyl phosphate

Cited by 37 publications

References 17 publications

Luminescent Aggregated Copper Nanoclusters Nanoswitch Controlled by Hydrophobic Interaction for Real-Time Monitoring of Acid Phosphatase Activity

Luminescent Aggregated Copper Nanoclusters Nanoswitch Controlled by Hydrophobic Interaction for Real-Time Monitoring of Acid Phosphatase Activity

A dual-signal fluorescent probe for detection of acid phosphatase

Label-Free Colorimetric Detection of Acid Phosphatase and Screening of Its Inhibitors Based on Biomimetic Oxidase Activity of MnO₂ Nanosheets

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High performance liquid chromatography equipped with a cathodic detector and column-switching device as a high-throughput method for a phosphatase assay with p-nitrophenyl phosphate

Cited by 37 publications

References 17 publications

Luminescent Aggregated Copper Nanoclusters Nanoswitch Controlled by Hydrophobic Interaction for Real-Time Monitoring of Acid Phosphatase Activity

Luminescent Aggregated Copper Nanoclusters Nanoswitch Controlled by Hydrophobic Interaction for Real-Time Monitoring of Acid Phosphatase Activity

A dual-signal fluorescent probe for detection of acid phosphatase

Label-Free Colorimetric Detection of Acid Phosphatase and Screening of Its Inhibitors Based on Biomimetic Oxidase Activity of MnO2 Nanosheets

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Product

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Label-Free Colorimetric Detection of Acid Phosphatase and Screening of Its Inhibitors Based on Biomimetic Oxidase Activity of MnO₂ Nanosheets