“…Various extraction methods such as affinity purification, adsorption-based separation, hydrophobic adsorbant, monolith column, and magnetic separation , have been developed to enrich trace CKs from complex matrix, but their selectivity to CKs is still a big challenge. Some analytical techniques including immunoassays, electroanalysis, capillary electrophoresis, liquid chromatography, , high/ultra performance liquid chromatography (HPLC/UPLC) coupled to different detectors, i.e., mass spectrometry (MS), have been developed for quantification of CKs. Compared with other methods, HPLC–MS and UPLC–MS/MS are the most powerful techniques because of its good resolution separation, sensitivity, and identification of CKs from plant samples.…”