High-Performance Liquid Chromatography-Diodic Array Detector, Fungistatic, and Anti-Morphogenical Analysis of Extracts from <i>Psidium brownianum</i> Mart. ex DC. Against Yeasts of the Genus <i>Candida</i>

Morais-Braga, Maria Flaviana Bezerra; Carneiro, Joara Nályda Pereira; Machado, Antônio Júdson Targino; Sales, Débora Lima; Brito, Dara Isabel Vieira de; Albuquerque, Rosimeire Sabino; Boligon, Aline Augusti; Athayde, Margareth Linde; Júnior, João Tavares Calixto; Souza, Djair S.L.; Lima, Edeltrudes O.; Menezes, Irwin Rose Alencar de; Costa, José G.M.; Ferreira, Felipe Silva; Coutinho, Henrique Douglas Melo

doi:10.1080/10942912.2015.1079786

Cited by 15 publications

(8 citation statements)

References 26 publications

(29 reference statements)

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“…Virulence inhibition of Candida species has already been observed both by the action of extracts from other species belonging to the Myrtaceae family as well as by the action of plant products derived from other families. Morais-Braga et al (2016;2017) reported in their study that the aqueous and hydroethanolic extracts from Psidium guajava and Psidium brownianum (Myrtaceae) leaves affected the fungal virulence of C. albicans and C. tropicalis at the 16.384 μg/mL concentration. Brito et al (2015), showed that the C. krusei and C. tropicalis strains cultivated in culture medium in the presence of the Lippia sidoides (Verbenaceae) essential oil did not present pseudohyphae and hyphae at the 256 μg/mL concentration.…”

Section: Antifungal Activitymentioning

confidence: 99%

Chemical composition, antifungal activity and potential anti-virulence evaluation of the Eugenia uniflora essential oil against Candida spp.

et al. 2018

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The development of fungal resistance to antifungal drugs has been worsening over the years and as a result research on new antifungal agents derived from plants has intensified. Eugenia uniflora L. (pitanga) has been studied for its various biological actions. In this study the chemical composition and antifungal effects of the E. uniflora essential oil (EULEO) were investigated against Candida albicans (CA), Candida krusei (CK) and Candida tropicalis (CT) standard strains. The essential oil obtained through hydro-distillation was analyzed by gas chromatography coupled to mass spectrometry (GC-MS). To determine the IC of the oil, the cellular viability curve and the inhibitory effects were measured by means of the oil's association with Fluconazole in a broth microdilution assay with spectrophotometric readings. The Minimum Fungicidal Concentration (MFC) was determined by solid medium subculture with the aid of a guide plate while the assays used to verify morphological changes emerging from the action of the fractions were performed in microculture chambers at concentrations based on the microdilution. Two major oil constituents stand out from the chemical analysis: selina-1,3,7(11)-trien-8-one (36.37%) and selina-1,3,7(11)-trien-8-one epoxide (27.32%). The concentration that reduced microorganismal growth was ≥8,192 μg/mL while the IC varied, this being between 1892.47 and 12491.80 μg/mL (oil), 10.07 - 80.78 μg/mL (fluconazole) and 18.53 - 295.60 μg/mL (fluconazole + oil). The combined activity (fluconazole + oil) resulted in indifference and antagonism. A MFC of the oil in association with fluconazole was recorded at the concentration of 8,192 μg/mL against CA and CK. The oil caused the inhibition of CA and CT morphological transition. In view of the results obtained, additional research is needed to elucidate the activity of the E. uniflora oil over genetic and biochemical processes regarding its effect on Candida spp. virulence.

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Section: Antifungal Activitymentioning

confidence: 99%

Chemical composition, antifungal activity and potential anti-virulence evaluation of the Eugenia uniflora essential oil against Candida spp.

et al. 2018

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“…C. albicans was selected for association study because it is the most common pathogenic agent involved in systemic infections and the main strain responsible for infections caused by Candida fungi ( Romani, 2012 ; Yapar, 2014 ). In the previous studies, of our research group, with other species of this genus, P. brownianum and P. guajava extracts had their antifungal potential investigated, obtaining favorable results, where they also managed to affect the phenotypic plasticity of C. albicans and C. tropicalis , reducing the formation process of hyphae and pseudohyphae as their concentrations were increased ( Morais-Braga et al, 2016b ; Morais-Braga et al., 2016a ; Morais-Braga et al., 2017 ).…”

Section: Discussionmentioning

confidence: 95%

“…Within the genus Psidium , some data exist; however, they are related to extracts. Morais-Braga et al. (2016a) and Morais-Braga et al.…”

Section: Discussionmentioning

confidence: 99%

“…A control for yeast growth (hyphae stimulated by depleting medium) was performed, as well as a control with the conventional antifungal fluconazole for comparative purposes. Tests using DMSO as a control were previously performed ( Morais-Braga et al, 2016a ), demonstrating that it does not cause inhibition of hyphae at the concentrations tested.…”

Section: Methodsmentioning

confidence: 97%

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Effect of seasonality on chemical profile and antifungal activity of essential oil isolated from leaves Psidium salutare (Kunth) O. Berg

Macêdo

Souza

Morais-Braga

et al. 2018

PeerJ

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Medicinal plants play a crucial role in the search for components that are capable of neutralizing the multiple mechanisms of fungal resistance. Psidium salutare (Kunth) O. Berg is a plant native to Brazil used as both food and traditional medicine to treat diseases and symptoms such as stomach ache and diarrhea, whose symptoms could be related to fungal infections from the genus Candida. The objective of this study was to investigate the influence of seasonal variability on the chemical composition of the Psidium salutare essential oil, its antifungal potential and its effect on the Candida albicans morphogenesis. The essential oils were collected in three different seasonal collection periods and isolated by the hydrodistillation process in a modified Clevenger apparatus with identification of the chemical composition determined by gas chromatography coupled to mass spectrometry (GC/MS). The antifungal assays were performed against Candida strains through the broth microdilution method to determine the minimum fungicidal concentration (MFC). Fungal growth was assessed by optical density reading and the Candida albicans dimorphic effect was evaluated by optical microscopy in microculture chambers. The chemical profile of the essential oils identified 40 substances in the different collection periods with γ-terpinene being the predominant constituent. The antifungal activity revealed an action against the C. albicans, C. krusei and C. tropicalis strains with an IC50 ranging from 345.5 to 2,754.2 µg/mL and a MFC higher than 1,024 µg/mL. When combined with essential oils at sub-inhibitory concentrations (MIC/16), fluconazole had its potentiated effect, i.e. a synergistic effect was observed in the combination of fluconazole with P.salutare oil against all Candida strains; however, for C. albicans, its effect was reinforced by the natural product in all the collection periods. The results show that the Psidium salutare oil affected the dimorphic transition capacity, significantly reducing the formation of hyphae and pseudohyphae in increasing concentrations. The results show that P. salutare oil exhibits a significant antifungal activity against three Candida species and that it can act in synergy with fluconazole. These results support the notion that this plant may have a potential use in pharmaceutical and preservative products.

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“…Fruits of species of the genus Psidium are widely consumed by the population and avifauna of the Caatinga biome in the Northeast of Brazil (LUCENA;MAJOR;BONILLA, 2015;MENDES;SOUZA, 2016). Several studies report the characteristics of essential oils of these species because they are very promising for antioxidant, antimicrobial, cytotoxic (SIMONETTI et al, 2016), and antifungal applications (MORAIS-BRAGA et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

SEED, SEEDLING AND FRUIT MORPHOLOGY AND SEED GERMINATION OF Psidium sobralianum PLANTS OF THE SÃO FRANCISCO VALLEY, BRAZIL

et al. 2018

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The Northeast region of Brazil has the second highest number of species of the Myrtaceae family. It is mostly covered by the Caatinga biome, which is very degraded, making it difficult to preserve species of this family. Thus, the objective of this work was to describe the seed, seedling, and fruit morphology, and seed germination of Psidium sobralianum Landrum & Proença plants of the São Francisco Valley, Brazil. The fruits were collected in an area of the Brazilian Agricultural Research Corporation (Embrapa Semiarid), in Petrolina PE, Brazil. The evaluations consisted of determinations of fruit shape, consistency, and number of seeds; seed form, cotyledons, hilum, hypocotyl-radicle axis, and embryo type; epicarp, mesocarp, endocarp, and seed staining; longitudinal, transversal, and ventral diameters of fruits and seeds; fruit, pulp, and seed fresh and dry masses; germination test; first count of germinated seeds; germination speed index; shoot and root lengths; shoot and root fresh and dry masses; and imbibition test. Psidium sobralianum has polyspermic, berry fruits, subclassified as solanidium, with persistent sepals and globular shape, consisting of epicarp, mesocarp, endocarp and seeds. The fruits have green with orange epicarp, pale-green mesocarp, and white endocarp. The seeds have a pilose and bony aspect, a pimentoid type of embryo, foliaceous cotyledons, presence of operculum and hilum, and pale-yellow tegument. The germination is epigeal phanerocotiledonar, with root protrusion from 26 days after sowing (DAS); it presents a short, glabrous, thick radicle, and a rounded, pale-green apex. The germination is slow, probably due to the mechanical barrier of the tegument, and stabilizes at 90 DAS.

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High-Performance Liquid Chromatography-Diodic Array Detector, Fungistatic, and Anti-Morphogenical Analysis of Extracts from Psidium brownianum Mart. ex DC. Against Yeasts of the Genus Candida

Cited by 15 publications

References 26 publications

Chemical composition, antifungal activity and potential anti-virulence evaluation of the Eugenia uniflora essential oil against Candida spp.

Chemical composition, antifungal activity and potential anti-virulence evaluation of the Eugenia uniflora essential oil against Candida spp.

Effect of seasonality on chemical profile and antifungal activity of essential oil isolated from leaves Psidium salutare (Kunth) O. Berg

SEED, SEEDLING AND FRUIT MORPHOLOGY AND SEED GERMINATION OF Psidium sobralianum PLANTS OF THE SÃO FRANCISCO VALLEY, BRAZIL

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