High-performance liquid chromatography coupled with electrospray tandem mass spectrometry (LC/MS/MS) method for the simultaneous determination of diazepam, atropine and pralidoxime in human plasma

Development and validation of novel RP-UPLC method for estimation of atropine sulphate in pharmaceutical dosage form

“…Atropine sulphate was eluted using different stationary phases such as C 18 , C 8 , phenyl, amino and cyano as well as different mobile phases containing buffers like phosphate, sulphate, and acetate with different pH (2)(3)(4)(5) and using organic modifiers like acetonitrile, methanol and ethanol in the mobile phase. The peak shape of the atropine sulphate was found to be symmetrical at 210 nm wavelength.…”

“…Atropine sulphate injection is official in Indian pharmacopeia, British Pharmacopeia and United States Pharmacopeia. Some methods for the determination of tropane alkaloids appearing in the literature are based on TLC [2][3], gas chromatography [4], LC--MS [5,6], high performance liquid chromatography [7--12], capillary zone electrophoresis [13,14], chiral separation [15], with fluorescence detection [16], with conductometric detection [17], cation exchange [18], ion-pair high performance chromatography [19]. To the best of our knowledge, there is no RP-UPLC method reported in literature for determination of atropine suphate.…”

Tropane alkaloid analysis by chromatographic and electrophoretic techniques: An update

“…Single ion monitoring enabled a LOQ of 0.5 ng/ml. The problem of appropriate dosage of atropine after intoxication by organophosphates was addressed by Abbara et al [91]. They used HPLC coupled with electrospray mass spectrometry for the simultaneous determination of three drugs often given simultaneously to victims of nerve gas intoxications.…”

Development of a simple and sensitive HPLC-MS/MS method for determination of diazepam in human plasma and its application to a bioequivalence study

“…[2] Previous studies have proposed several analytical methods using high-performance liquid chromatography (HPLC) with an ultraviolet (UV) [3,4] or mass spectrometry (MS) [5][6][7][8][9] detection to determine the concentrations of diazepam administered by various routes including nasal spray, rectal gel, intramuscular injection, and oral formulations. [10][11][12][13][14][15] However, these previously developed analytical methods have been associated with several limitations including inadequate sample preparation due to difficulty in removing impurities, [5] inadequate sensitivity (a lower limit of quantitation [LLOQ] up to 50 ng/mL), [6] labor-intensive sample preparation process, [7] the need for a relatively large volume of plasma (≥ 100 μL) for analysis, [8] and long retention times (≥ 8 min). [9] Therefore, a new bioanalytical method to quantitate diazepam has been needed to improve those limitations.…”