High‐performance liquid chromatography–based fingerprint analysis with chemical pattern recognition for evaluation of Mahonia bealei (Fort.) Carr

Abstract

A simple and efficient high‐performance liquid chromatography method combined with chemical pattern recognition was established for quality evaluation of Mahonia bealei (Fort.) Carr. A common pattern of 30 characteristic peaks was applied for similarity analysis, hierarchical cluster analysis, principal component analysis, and partial least squares discriminant analysis in the 37 batches of M. bealei (Fort.) Carr. to discriminate wild M. bealei (Fort.) Carr., cultivated M. bealei (Fort.) Carr., and its substitutes. The results showed that partial least squares discriminant analysis was the most effective method for discrimination. Eight characteristics peaks with higher variable importance in projection values were selected for pattern recognition model. A permutation test and 26 batches of testing set samples were performed to validate the model that was successfully established. All of the training and testing set samples were correctly classified into three clusters (wild M. bealei (Fort.) Carr., cultivated M. bealei (Fort.) Carr., and its substitutes) based on the selected chemical markers. Moreover, 26 batches of unknown samples were used to predict the accuracy of the established model with a discrimination accuracy of 100%. The obtained results indicated that the method showed great potential application for accurate evaluation and prediction of the quality of M. bealei (Fort.) Carr.

Determination of Cytotoxic Activity of Selected Isoquinoline Alkaloids and Plant Extracts Obtained from Various Parts of Mahonia aquifolium Collected in Various Vegetation Seasons

“…For determination of isoquinoline alkaloids contents in various Mahonia species extracts high performance liquid chromatography (HPLC) was often applied [19]. Most often analysis were performed on octadecyl (C18) columns with mobile phases contained organic modifier (most often acetonitrile or methanol), water and addition of acids, e.g., phosphoric acid [19], formic acid [20,21], buffer at acidic pH [22], and addition of salts, e.g., sodium dihydrogen phosphate [2]. Capillary high performance liquid chromatography was also applied for analysis of alkaloids from Mahonia aquifolium extract [23].…”

“…Various detection mode were applied for detection of alkaloids in Mahonia species. Most often diode array detection (DAD) [19,22], mass spectrometry (MS) or tandem mass spectrometry (MS/MS) [20], DAD, and MS/MS [23] were used for this purpose. The application of various detection methods allowed the detection of alkaloids in extracts at different levels of their concentrations.…”

Application of fingerprint combined with quantitative analysis and multivariate chemometric methods in quality evaluation of dandelion (Taraxacum mongolicum)

“…The combination of characteristic fingerprint and QAMS by multivariate chemometric methods (MCM) was used to compare the similarity of dandelion fingerprint. MCM were established by hierarchical cluster analysis (HCA) and factor analysis (FA) [ 25 ], and HCA was especially performed using the R language and SPSS 22.0 software.…”

Simultaneous Determination of Four Bioactive Alkaloid Components in the Stems of Three <i>Mahonia</i> Plant Species by HPLC

“…The exhaustion of such resources has led to imbalance between the supply and demand of this crude medicine. The main bioactive components of Mahoniae caulis are alkaloids such as columbamine, jateorhizine, palmatine, and berberine [5,6] Mahonia confusa Sprague, which belongs to the same genus, is also used as a substitute source of Mahoniae caulis in Tujia Nationality residence areas (e.g., Hubei Province, China). Some documents described that the dried stem of M. confusa has similar efficiencies, pharmacological characteristics, and effective material basics to those of M. bealei and M. fortunei.…”