Application of fingerprint combined with quantitative analysis and multivariate chemometric methods in quality evaluation of dandelion (Taraxacum mongolicum)

Abstract

A quality assessment method based on quantitative analysis of multi-components by single marker (QAMS) and fingerprint was constructed from 15 batches of dandelion ( Taraxacum mongolicum ), using multivariate chemometric methods (MCM). MCM were established by hierarchical cluster analysis (HCA) and factor analysis (FA). HCA was especially performed using the R language and SPSS 22.0 software. The relative correction factors of chlorogenic acid, caffeic acid, p-coumaric acid, luteolin and apigenin were calculated with cichoric acid as a reference, and their contents were determined. The differences between external standard method (ESM) and QAMS were compared. There was no significant difference ( t -test, p > 0.05) in quantitative determination, proving the consistency of the two methods (QAMS and ESM). Dandelion material from Yuncheng, Shandong was used as a reference chromatogram. The fingerprints in 15 batches of dandelion were established by HPLC analysis. The similarity of the fingerprints in different batches of dandelion material was greater than or equal to 0.82. A total of 10 common peaks were identified. This strategy is simple, rapid and efficient in multiple component detection of dandelion. It is beneficial in simplifying dandelion's quality control processes and providing references to enhance quality control for other herbal medicines.

Study of Dandelion (Taraxacum mongolicum Hand.-Mazz.) Salt Response and Caffeic Acid Metabolism under Saline Stress by Transcriptome Analysis

“…Caffeic acid (µg/g), ρ-coumaric acid (µg/g), ferulic acid (µg/g), and caffeoyl shikimic acid contents (µg/g) were determined based on the method described in [13,14]. Dandelion leaves were dried at 80 • C in an oven, and then were soaked in 80% methanol with a liquid-to-solid ratio of 15:1 and ultrasonic-extracted for 30 min.…”

Integrated Microbiome-Metabolomics Analysis Reveals the Potential Mechanism of Dandelion Root Polysaccharides to Ameliorate Ulcerative Colitis

“…Documented in ancient texts such as the Compendium of Materia Medica, dandelion has been recognized for its dual utility as both a nutritional and medicinal herb [1]. This plant is celebrated for its comprehensive therapeutic benefits including, but not limited to, anti-cancer, anti-inflammatory, immunomodulatory, and antibacterial properties, in addition to its ability to alleviate swelling and disperse nodules [2].…”

Dandelion (Taraxacum Genus): A Review of Chemical Constituents and Pharmacological Effects

“…As an edible medicinal herb and vegetable, dandelion ( Taraxacum genus) has long been utilized in traditional medicine, folk remedies, and substitution therapies in many countries to treat diverse diseases ( Figure 1 ) [ 3 ]. Taraxacum genus as a drug was first used to treat liver and spleen diseases in Arabian medicine.…”