High-performance Liquid Chromatography/Atmospheric Pressure Chemical Ionization Mass Spectrometry with Ionization Polarity Switching for the Determination of Selected Pesticides

Barnes, Karen A.; Fussell, Richard J.; Startin, James R.; Pegg, Michael K.; Thorpe, S. A.; Reynolds, S. L.

doi:10.1002/(sici)1097-0231(19970115)11:1<117::aid-rcm781>3.0.co;2-v

Cited by 66 publications

(23 citation statements)

References 7 publications

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“…As far as crude sample extract is analysed, matrix components co-eluting with target analyte may in some extent suppress the ionisation process occurring in APCI, and consequently the limit of quantitation is increased. (Kebarle & Tang 1993;Barnes et al 1997;Choi et al 2001;Zrostlíková et al 2002Zrostlíková et al , 2003. Attempting to analyse crude poppy seeds extract, response suppression as low as 20% was observed as compared to area of isoproturon standard in neat solvent.…”

Section: Optimisation and Methods Performance Characteristicsmentioning

confidence: 68%

HPLC-MS/MS method for analysis of isoproturon in difficult matrix: poppy seeds

Kovalczuk¹,

Poustka²,

Hajšlová³

2008

Czech J. Food Sci.

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Kovalczuk T., Poustka J., Hajšlová J. (2008): HPLC-MS/MS method for analysis of isoproturon in difficult matrix: poppy seeds. Czech J. Food Sci., 26: 146-152.While several validated methods have been developed for analysis of phenylurea herbicides in staple food plants, analytical procedures suitable for their quantification in minor crops such as poppy seeds are not available. For the registration of isoproturon use in this crop, the documentation of dynamics of its residues following treatment was requested. To accomplish this task, HPLC-MS/MS method was developed. Extraction of residues was realised by methanol-water mixture, Supelclean LC-18 SPE cartridges were used for purification of crude extracts. For HPLC separation of isoproturon SPE fraction Lichrospher C18 column (25 cm × 4 mm, 5 µm) was employed. For detection and quantification of target analyte, mass spectrometer with ion trap analyser operated in positive atmospheric pressure chemical ionisation (APCI) and MS/MS mode was used. Following performance parameters of method were obtained: detection limit 0.01 mg/kg, recovery 84%, and repeatability 7%.

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Section: Optimisation and Methods Performance Characteristicsmentioning

confidence: 68%

HPLC-MS/MS method for analysis of isoproturon in difficult matrix: poppy seeds

Kovalczuk¹,

Poustka²,

Hajšlová³

2008

Czech J. Food Sci.

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“…In all cases, these LODs and LOQs were significantly lower than those reported by other techniques without column switching mode, such as GC-ECD [37], LC-UV [18,20,21] or PIF [38,39] and are in the same order as those obtained by LC-PIF-FD [22] and LC-MS [23][24][25], using a SPE step in all cases.…”

Section: Validation Of the Lc-lc-pif-fd Methodsmentioning

confidence: 49%

“…Benzoylurea residues are generally analyzed by LC using UV [18][19][20][21], FD [22], or MS detection [23][24][25]. Because of their high polarity and poor detectability using any kind of detector, these methodologies require considerable effort in sample preparation steps such as extraction, concentration, and clean-up techniques.…”

Section: Introductionmentioning

confidence: 99%

Coupled‐column liquid chromatography method with photochemically induced derivatization for the direct determination of benzoylureas in vegetables

García

Martínez

Galera

et al. 2004

J of Separation Science

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The coupled-column (LC-LC) system, consisting of a first column packed with internal surface reversed phase (ISRP) (50x4.6 mm ID) and a Chrompack C18 (100x4.6 mm ID) as second column, allowed the simultaneous determination of five benzoylurea insecticides in dichloromethane (CH 2 Cl 2 ) extracts of vegetable samples without any clean-up step. This system was combined with a photochemically induced fluorescence (PIF) post-column derivatization in order to provide strongly fluorescent photoproducts from the non-fluorescent benzoylureas. Limits of detection ranged from 0.21 to 0.98 g L -1 of pesticide (equivalent to 0.14-0.65 g kg -1 in vegetable samples) and limits of determination ranged from 4.0 to 10.0 g L -1 (equivalent to 2.7-6.7 g kg -1 ). Linearity of the method was established between 2 and 1800 g L -1 , depending upon the compound.Validation of the total method was performed by randomly analyzing recoveries of four vegetable samples (aubergine, cucumber, green bean, and tomato) spiked at two levels of concentration (10.0 and 33.3 g kg -1 ). The combination of the LC-LC system with PIF detection provides a sensitive, selective, and rapid method for the determination of pesticides in vegetable samples at levels lower than the maximum residue levels (MRLs) established for these compounds by Spanish legislation.

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“…In order to select the most suitable LC/MS technique, an initial comparison between APCI and ES was made. 53 Spectra of the selected compounds were acquired using APCI and ES in both PI and NI modes, obtaining similar spectra, although for most compounds with ES the most significant ion was the sodium adduct ion. Limits of detection were comparable for most compounds with the two techniques, but APCI was more sensitive for aldicarb and its metabolites.…”

Section: Atmospheric Pressure Chemical Ionization Interfacementioning

confidence: 99%

High‐Performance Liquid Chromatography/Mass Spectrometry Methods in Pesticide Analysis

Cappiello

Palma

2000

Encyclopedia of Analytical Chemistry

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In this article, a detailed overview of the current high‐performance liquid chromatography/mass spectrometry (HPLC/MS) instrumentation, suitable for the determination of a large number of pesticides, is reported. Particular emphasis is given to the role of the mass spectrometric apparatus involved in each interfacing technique with several types of information regarding the ionization processes, analyzer principles and system configurations. The article gives an up‐to‐date picture of the current instrumentation that is available and readers will find only brief references to some important historical approaches that are no longer in use.

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High-performance Liquid Chromatography/Atmospheric Pressure Chemical Ionization Mass Spectrometry with Ionization Polarity Switching for the Determination of Selected Pesticides

Cited by 66 publications

References 7 publications

HPLC-MS/MS method for analysis of isoproturon in difficult matrix: poppy seeds

HPLC-MS/MS method for analysis of isoproturon in difficult matrix: poppy seeds

Coupled‐column liquid chromatography method with photochemically induced derivatization for the direct determination of benzoylureas in vegetables

High‐Performance Liquid Chromatography/Mass Spectrometry Methods in Pesticide Analysis

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