HPLC-MS/MS method for analysis of isoproturon in difficult matrix: poppy seeds

Abstract

Kovalczuk T., Poustka J., Hajšlová J. (2008): HPLC-MS/MS method for analysis of isoproturon in difficult matrix: poppy seeds. Czech J. Food Sci., 26: 146-152.While several validated methods have been developed for analysis of phenylurea herbicides in staple food plants, analytical procedures suitable for their quantification in minor crops such as poppy seeds are not available. For the registration of isoproturon use in this crop, the documentation of dynamics of its residues following treatment was requested. To accomplish this task, HPLC-MS/MS method was developed. Extraction of residues was realised by methanol-water mixture, Supelclean LC-18 SPE cartridges were used for purification of crude extracts. For HPLC separation of isoproturon SPE fraction Lichrospher C18 column (25 cm × 4 mm, 5 µm) was employed. For detection and quantification of target analyte, mass spectrometer with ion trap analyser operated in positive atmospheric pressure chemical ionisation (APCI) and MS/MS mode was used. Following performance parameters of method were obtained: detection limit 0.01 mg/kg, recovery 84%, and repeatability 7%.

Determination of Isoproturon in Environmental Samples using the QuEChERS Extraction‐Spectrofluorimetric Method

“…The data in Table show that the proposed method is comparable to other reported methods (Irace‐Guigand et al 2005; Pulido‐Tofino et al ; Kovalczuk et al ; Yang et al ) in terms of accuracy and sensitivity; in terms of simplicity and cost‐effectiveness, our proposed method is superior to other reported methods and is useful for routine analysis of isoproturon in a variety of samples.…”

“…A literature method (Kovalczuk et al 2008) with some modifications was employed for extraction of isoproturon from poppy seeds and mustard seeds. Exactly 20 mL of a CH 3 OH: H 2 O (1:1, v/v) mixture was added to each of the samples followed by shaking the suspensions on an orbital shaker for 30 min at 150 rpm.…”

“…3-(4-Isopropylphenyl)-1,1-dimethylurea (isoproturon) is marketed under the trade names of Ipuron, Alon, Guideline, Panron, Passport, and many others. It is a systemic and selective herbicide sprayed against wild oats, silky bent grass, black grass, ray grass, annual meadow grass, and many broadleaf weeds among spring and winter wheat, winter rye, and spring and winter barley (Winrow et al 2003;Zhang et al 2006;Kovalczuk et al 2008). Being slightly soluble in water, isoproturon enters the human food chain through soil and crops (Krämer et al 2004).…”