High-performance liquid chromatography and capillary electrophoresis for the analysis of maize proteins

Abstract

ReviewHigh-performance liquid chromatography and capillary electrophoresis for the analysis of maize proteinsMethods for the analysis of maize proteins using HPLC and CE are reviewed. Most of the references cited in this review concern HPLC methods. Size-exclusion HPLC and especially RP-HPLC methods have been developed for characterization of normal and genetically modified maize, cultivar differentiation, and prediction of quality. Few CE methods for the analysis of maize proteins were found in the existing literature. Most of these methods focus on optimization of the separation of maize proteins using CZE and SDS-capillary gel electrophoresis.

Modification of Resolution in Capillary Electrophoresis for Protein Profiling in Identification of Genetic Modification in Foods

“…Among employed modifications of BGE with dendrimers or coating capillary wall with polybrene, the latter method seems to be more appropriate providing better reproducibility and more pronounced differences in protein profiles. The observed differences in analyzed samples show some potential possibilities of application of this concept for identification of GMO, which is consistent with some earlier works using HPLC [37][38][39] and CE measurements [17][18][19][20] of protein profiles. However, this should Croat.…”

Capillary electrophoresis of proteins 2005–2007

“…The use of CE for identifying grain variety and quality assurance has been reviewed [110,111] as well as CE analysis of maize proteins [112].…”

CE‐MS of zein proteins from conventional and transgenic maize

“…HPLC and CE have also been used to separate zein proteins [8]. Thus, proteins can be separated by their affinity to a stationary phase, by their pI or by their electrophoretic mobility.…”

“…This might be due to the high tendency of zein proteins to form aggregates through disulfide and hydrogen bridge bonds. For this reason, most separations are performed either under reducing condition or using surfactants (e.g., SDS) [8] especially in CE, making highly incompatible the separation step with the subsequent MS analysis.…”