High-performance liquid chromatographic study of the photochemical oxidation of vasicine and its analogues

Chowdhury, B. K.; Hirani, S. K.; Ngur, Dan O.

doi:10.1016/s0021-9673(01)94396-1

Cited by 14 publications

(8 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, it was demonstrated that Nitrogen base fertilizer caused a significant increase in the vegetative growth, essential oil, fixed oil, total carbohydrates, soluble sugars plants (Zheljazkov and Margina, 1996;Khalid, 2001). Physicochemical constituent such as total ash provides information on the mineral compo- than 30% of crude extract (Chowdhuri et al, 1987).…”

Section: Discussionmentioning

confidence: 99%

Growth and phytochemical composition of adhatoda zeylanica in response to foliar application of growth hormones and urea

Tandel

Animasaun

Krishnamurthy

2018

J. Soil Sci. Plant Nutr.

View full text Add to dashboard Cite

This study evaluates effects of foliar application of Kinetin, Benzyladenine and urea on growth, phytochemical and quality of active compound vacisine in Adhatoda zeylanica. Five treatments; distilled water (T 1 ) which serves as control, 0.1% urea + 100 ppm kinetin (T 2 ), 0.1 % urea + 100 ppm Benzyladenine (T 3 ), 0.2% urea + 100 ppm kinetin (T 4 ) and 0.2 % urea + 100 pm Benzyladenine (T 5 ) were applied as foliar spray every four weeks. Growth parameters, phytochemical screening and physicochemical properties of the treated plants were evaluated. Also, bioactive compound vicisine from the leaf extract was characterised and compared with standard using High Performance Thin Layer Chromatography (HPTLC). All the treatments had better growth performances than T1, however, in term of yield, T2 has optimum effect. Phytochemical screening reveal presence of alkaloid, tannin, phenolic compounds, steroids, proteins and carbohydrates. Water and alcohol solubility, pH, and total ash values were enhance by the treatments. HPTLC analysis showed that the leaf extract active compound vicisine had same quality as the market standard. The study concludes that foliar application of growth hormones and urea in combinations and different concentrations could be used to improve growth and phytochemical yield of medicinal plants.

show abstract

Section: Discussionmentioning

confidence: 99%

Growth and phytochemical composition of adhatoda zeylanica in response to foliar application of growth hormones and urea

Tandel

Animasaun

Krishnamurthy

2018

J. Soil Sci. Plant Nutr.

View full text Add to dashboard Cite

show abstract

“…The medicinal properties of AB and AV are due to the presence of pyrroquinazoline alkaloids (tricyclic quinazoline alkaloids), viz. vasicine and vasicinone (Brain & Thapa, ; Chowdhuri & Hirani, ; Chihara, ; Singh, Vadmavathi, & Rao, ; Zabeer et al, ; Lone et al, ; Srinivasan et al, ). AV is reported to be the richest source of tricyclic quinazoline alkaloids (Dyakonov & Telezhenetskaya, ).…”

Section: Introductionmentioning

confidence: 99%

“…Several analytical methods are reported for the quantification of vasicine and vasicinone in AV extract. HPTLC, TLC, HPLC-UV and capillary electrophoresis are used to obtain the content of vasicine (Brain & Thapa, 1983;Chowdhuri & Hirani, 1987;Lone et al, 2013;Farooqui et al, 2014;Madhukar et al, 2014). From the above-mentioned techniques, vasicine was reported as a major bioactive pyrroquinazoline alkaloid of AV leaf in the range of 0.05-2.08% w/w (Brain & Thapa, 1983;Soni, Anandjiwala, Patel, & Rajani, 2008;Farooqui et al, 2014;Madhukar et al, 2014).…”

mentioning

confidence: 99%

“…From the above-mentioned techniques, vasicine was reported as a major bioactive pyrroquinazoline alkaloid of AV leaf in the range of 0.05-2.08% w/w (Brain & Thapa, 1983;Soni, Anandjiwala, Patel, & Rajani, 2008;Farooqui et al, 2014;Madhukar et al, 2014). The UHPLC-ESI-MS/MS analytical method in multiple reaction monitoring (MRM) mode is a more powerful approach compared with other reported analytical methods (Chowdhuri & Hirani, 1987;Soni et al, 2008;Singh et al, 2015a). This method rapidly quantifies multiple components in a complex matrix owing to its rapid separation power, good sensitivity, high specificity and short run time (Qian et al, 2015).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous determination of pyrroquinazoline alkaloids and flavonoids in Adhatoda beddomei and Adhatoda vasica and their marketed herbal formulations using ultra‐high‐performance liquid chromatography coupled with triple quadrupole linear ion trap mass spectrometry

Singh

Kumar

Bajpai

et al. 2016

Biomedical Chromatography

View full text Add to dashboard Cite

Adhatoda beddomei and Adhatoda vasica leaf, known as 'Vasaka' and/or 'Vasa' in Ayurveda and 'Malabar nut' in English, is an official drug in the Indian Pharmacopoeia. The medicinal properties of these plants are due to the presence of pyrroquinazoline alkaloids. An UHPLC-ESI/MS/MS method in both positive and negative electrospray ionization in multiple-reaction-monitoring mode was developed and validated for the estimation of alkaloids and flavonoids in Adhatoda species and their marketed herbal formulations. Chromatographic separation was achieved on an Acquity UPLC® BEH C -column using a gradient elution with 0.1% formic acid in water and methanol. The developed method was validated as per International Conference on Harmonization guidelines and found to be accurate with overall recovery in the range 94.2-105.0% (RSD ≤ 1.71%), precise (RSD ≤ 3.44%) and linear (R ≥ 0.9992) over the concentration range of 0.5-1000 ng/mL. The total content of alkaloids and flavonoids were highest in the chloroform and aqueous fraction of A. vasica leaf, respectively. The results indicated that the developed method was simple, rapid, sensitive, selective and accurate for the estimation of multiple bioactive constituents in crude mixture, and therefore could make a contribution to the quality control of Adhatoda species and its derived herbal formulations.

show abstract

“…[12] Vasicine is a light sensitive compound and when exposed to light, it is auto-oxidized to vasicinone. [1920] Both vasicine and vasicinone are chief alkaloids known to possess interesting biological activities. [212223] Vasicine and vasicinone are quinazoline alkaloids known to possess bronchodilatory activity; the first was reported to be more potent and also showed anti asthmatic activity.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of vasicine and vasicinone by high-performance liquid chromatography in roots of eight Sida species

Subramanya¹,

Pai²,

Ankad

et al. 2016

AYU

View full text Add to dashboard Cite

Introduction:Sida L. is a medicinally important genus widely used in conventional systems of medicine in India.Aim:The present study aims toward simultaneous determination of two bioactive compounds vasicine and vasicinone in root extracts of eight Sida spp. from Western Ghats, India.Materials and Methods:Determination of vasicine and vasicinone was undertaken in methanolic root extracts (10% w/v) of Sida acuta, Sida cordata, Sida cordifolia, Sida rhombifolia, Sida spinosa, Sida indica, Sida retusa and Sida mysorensis by high performance liquid chromatography (HPLC) method. The standards were prepared with the concentration of mg/mL. Data were expressed as mean values of three reading and relative standard deviations. The separation was achieved on a Waters, Nova-Pack, C18 (250 mm × 4.6 mm, 5 μ) column, with acetonitrile - 0.1 M phosphate buffer-glacial acetic acid (15: 85: 1, v/v/v) as solvent system at a flow-rate of 1.0 mL/min. The effluent was monitored using ultraviolet detection at a wavelength of 300 nm.Results:Both calibration curves of standard showed good linear regression (R2 > 0.994). The limit of detection and the limit of quantification for vasicine was 0.110 and 0.333 μg/mL and for vasicinone was 0.059 and 0.179 μg/mL respectively. The vasicine content was highest in S. cordifolia (9.891 ± 0.495 μg/100 mg) and vasicinone content was rich in S. cordata (33.013 ± 1.651 μg/100 mg.) The content of vasicinone was higher than vasicine.Conclusion:HPLC method provides simple, accurate, and reproducible quantitative analysis for simultaneous determination of vasicine and vasicinone. Among the selected Sida species, S. cordifolia and S. cordata were found to be rich in the vasicine and vasicinone contents, respectively.

show abstract

High-performance liquid chromatographic study of the photochemical oxidation of vasicine and its analogues

Cited by 14 publications

References 5 publications

Growth and phytochemical composition of adhatoda zeylanica in response to foliar application of growth hormones and urea

Growth and phytochemical composition of adhatoda zeylanica in response to foliar application of growth hormones and urea

Simultaneous determination of pyrroquinazoline alkaloids and flavonoids in Adhatoda beddomei and Adhatoda vasica and their marketed herbal formulations using ultra‐high‐performance liquid chromatography coupled with triple quadrupole linear ion trap mass spectrometry

Simultaneous determination of vasicine and vasicinone by high-performance liquid chromatography in roots of eight Sida species

Contact Info

Product

Resources

About