Growth and phytochemical composition of adhatoda zeylanica in response to foliar application of growth hormones and urea

Abstract

This study evaluates effects of foliar application of Kinetin, Benzyladenine and urea on growth, phytochemical and quality of active compound vacisine in Adhatoda zeylanica. Five treatments; distilled water (T 1 ) which serves as control, 0.1% urea + 100 ppm kinetin (T 2 ), 0.1 % urea + 100 ppm Benzyladenine (T 3 ), 0.2% urea + 100 ppm kinetin (T 4 ) and 0.2 % urea + 100 pm Benzyladenine (T 5 ) were applied as foliar spray every four weeks. Growth parameters, phytochemical screening and physicochemical properties of the treated plants were evaluated. Also, bioactive compound vicisine from the leaf extract was characterised and compared with standard using High Performance Thin Layer Chromatography (HPTLC). All the treatments had better growth performances than T1, however, in term of yield, T2 has optimum effect. Phytochemical screening reveal presence of alkaloid, tannin, phenolic compounds, steroids, proteins and carbohydrates. Water and alcohol solubility, pH, and total ash values were enhance by the treatments. HPTLC analysis showed that the leaf extract active compound vicisine had same quality as the market standard. The study concludes that foliar application of growth hormones and urea in combinations and different concentrations could be used to improve growth and phytochemical yield of medicinal plants.

Combined effects of NPK fertilizer with foliar application of benzyladenine or gibberellic acid on Dracaena marginata ‘Bicolor’ grown in different potting media

“…Similar results on increasing the contents of chlorophylls or carbohydrates due to application the combined NPK with GA 3 were reported by previous study (El-Sayed et al, 2016;Ghatas, 2016). Earlier studies (Tandel et al, 2018) reported increase in carbohydrates content due to combined urea with BA.…”

“…The Least Significant Difference (L.S.D.) test at the 5% level was used to compare the means values of different parameters (Steel and Torrie, 1997).…”

“…It was stated that the increase of plant growth by BA mainly associated with higher net assimilation rates and increased N content per unit leaf area (Di Benedetto and Galmarini, 2015), while increasing plant growth due to GA 3 may be attributed to its influence on increasing photosynthesis rate through increasing leaf surface (Lima et al, 2014). Moreover, increasing plant growth as a result of combined growth hormones with nutrient may be owing to physiological role of hormones and nutrient in synthesis of the plant phytochemicals through the action of various enzymes activity and protein synthesis (Tandel et al, 2018).…”

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“…In the current study, Adhatoda zeylanica plant extracts also showed the presence of various phytochemicals such as alkaloids, tannins, saponins, steroids, flavonoids, carbohydrates, polyphenols, proteins, and amino acids. Our present study results were agreed with past study of Tandel et al, (2018) 19 . In the past study, the authors have conducted experiments on ethanol extract of Adhatoda zeylanica plant leaves, and they found the presence of alkaloids, tannins, steroids, carbohydrates, polyphenols, and proteins like present study results.…”

Standardization of Agro-techniques for Melaleuca bracteata F. Muell

“…The number of leaves also increased with the application of BAP by Eid and Abou-Leila [11] on croton. Moreover, increasing plant growth as a result of combined growth hormones with nutrient may be owing to physiological role of hormones and nutrient in synthesis of the plant phytochemicals through the action of various enzymes activity and protein synthesis [12].…”