High-performance liquid chromatographic separation of caffeine, theophylline, theobromine and paraxanthine in rat brain and serum

Parra, Pilar; Limon, A. Williams; Ferré, Sergi; Guix, Teresa; Jané, F

doi:10.1016/0378-4347(91)80214-w

Cited by 20 publications

(4 citation statements)

References 22 publications

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“…Our results are not expected to differ from those obtained by other methods of administration, such as oral or subcutaneous, because they show similar pharmacokinetic curves in plasma (8). Furthermore, the selected dose is in accordance with previous studies (16,17,21,23).…”

Section: Discussionsupporting

confidence: 69%

Different brain structures exhibit the same caffeine levels after the administration of a single dose of caffeine

et al. 2016

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Caffeine is a highly consumed stimulant of the nervous system. Although caffeine has diverse effects on different brain functions, little is known about the specific pharmacokinetics of this substance in the brain. For instance, most studies that assessed caffeine distribution in the rat brain have only measured caffeine levels in the cortex and striatum but not in more specific brain areas.Aims: The purpose of this work was to measure the caffeine concentration in blood and different brain regions (i.e. cortex, striatum, hippocampus, cerebellum and brainstem) at different times after the administration of a single intraperitoneal dose of caffeine.Methods: Adult Wistar rats (250 to 300 gr) were injected with a single intraperitoneal dose of 30 mg/ Kg of caffeine. 20, 40, 60 and 80 minutes after administration, subjects were sacrificed by decapitation and samples of plasma, cerebral cortex, striatum, hippocampus, cerebellum and brainstem were obtained. Caffeine levels in the blood and each brain structure were DIFFERENT BRAIN STRUCTURES EXHIBIT THE SAME CAFFEINE LEVELS AFTER THE ADMINISTRATION OF A SINGLE DOSE OF CAFFEINE K e y w o r d s : e b r a i n , c a f f e i n e , H P L C , pharmacokinetics, plasma.Como citar este artículo: Orrego M, Jiménez-Rodriguez A, Osorio-Forero A, Restrepo F, Arias JM, Tamayo-Orrego L. Different brain structures exhibit the same caffeine levels after the administration of a single dose of caffeine.

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Section: Discussionsupporting

confidence: 69%

Different brain structures exhibit the same caffeine levels after the administration of a single dose of caffeine

et al. 2016

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“…Plasma concentrations of alcohol and caffeine were assayed by immunofluorescence [13] and h.p.l.c. [14] methods, respectively. The inter-assay coefficients of variation were lower than 7%.…”

Section: Alcohol and Caffeine Plasma Concentrationsmentioning

confidence: 99%

Evaluation of the central effects of alcohol and caffeine interaction.

Azcona

Barbanoj

Torrent

et al. 1995

Brit J Clinical Pharma

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1. The dynamic and kinetic interactions of alcohol and caffeine were studied in a double‐blind, placebo controlled, cross‐over trial. Treatments were administered to eight healthy subjects in four experimental sessions, leaving a 1 week wash‐out period between each, as follows: 1) placebo, 2) alcohol (0.8 g kg‐1), 3) caffeine (400 mg) and 4) alcohol (0.8 g kg‐1) + caffeine (400 mg). 2. Evaluations were performed by means of: 1) objective measures: a) psychomotor performance (critical flicker fusion frequency, simple reaction time and tapping test), b) long latency visual evoked potentials ('pattern reversal'); 2) subjective self‐rated scales (visual analogue scales and profile of mood states); 3) caffeine and alcohol plasma concentration determinations. 3. The battery of pharmacodynamic tests was conducted at baseline and at +0.5 h, +1.5 h, +2.5 h, +4 h and +6 h. An analysis of variance was applied to the results, accepting a P < 0.05 as significant. The plasma‐time curves for caffeine and alcohol were analysed by means of model‐independent methods. 4. Results obtained with caffeine in the objective measures demonstrated a decrease in simple reaction time and an increase in the amplitude of the evoked potentials; the subjects' self‐ratings showed a tendency to be more active. Alcohol increased simple reaction time and decreased amplitude of the evoked potentials, although the subjects rated themselves as being active. The combination of alcohol + caffeine showed no significant difference from placebo in the objective tests; nevertheless, the subjective feeling of drunkenness remained. The area under the curve (AUC) for caffeine was significantly higher when administered with alcohol.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…The reversed-phase liquid chromatography (LC) with UV absorbance detection is the most commonly separation technique used for this purpose. [6][7][8][9][10][11][12][13] However, the application of LC coupled with mass spectrometric detection 14 and amperometric detection 15 have also been reported for the analysis of methylxanthines. The isolation of theophylline, theobromine and caffeine could also be carried out by ion-pair, or ion-interaction, or ion chromatography coupled with UV detection.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of theophylline, theobromine and caffeine in different tea beverages by graphene-oxide based ultrasonic-assisted dispersive micro solid-phase extraction combined with HPLC-UV

et al. 2014

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A simple ultrasound-assisted dispersive micro-solid phase extraction method based on graphene-oxide (GO) nanosorbents (UAD-m-SPE), followed by high performance liquid chromatography-ultraviolet detection (HPLC-UV) was developed for the simultaneous determination of theophylline, theobromine and caffeine in different infusion tea samples. GO was synthesized by Hummers method and characterized with field emission-scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), and Fourier transform infrared (FT-IR) spectroscopy. The GO sorbent was dispersed in the sample solution with the assistance of ultrasound to enhance adsorption and desorption performance and rates. The main parameters influencing the extraction efficiency, including desorption solvent (type and volume), sorbent dosage, adsorption time, desorption time, and ionic strength were studied and optimized by a one factor at a time method. The best performance of the method was achieved with 3 mg mL À1 of GO, adsorption time of 15 min, desorption time of 4 min, salt concentration of 5%, and 100 mL of desorption solvent (alkaline methanol). Under the optimized conditions, the limit of detection (LOD) and limit of quantification (LOQ) were attained in the ranges 0.11-0.90 ng mL À1 and 0.37-3.00 ng mL À1 , respectively. The method showed a good linearity in the range of 0.003-5 mg mL À1 with a coefficient of determination (R 2 ) higher than 0.986. The repeatability and reproducibility of the method were 1.80% and 2.9%, respectively. The developed technique (GO-UAD-m-SPE) was successfully applied to the determination of the analytes in beverage tea samples including black, white, oolong, and green teas with the relative recoveries in the range of 84.6-113.6%.

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High-performance liquid chromatographic separation of caffeine, theophylline, theobromine and paraxanthine in rat brain and serum

Cited by 20 publications

References 22 publications

Different brain structures exhibit the same caffeine levels after the administration of a single dose of caffeine

Different brain structures exhibit the same caffeine levels after the administration of a single dose of caffeine

Evaluation of the central effects of alcohol and caffeine interaction.

Simultaneous determination of theophylline, theobromine and caffeine in different tea beverages by graphene-oxide based ultrasonic-assisted dispersive micro solid-phase extraction combined with HPLC-UV

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