High-Performance Liquid Chromatographic Quantification of Flavonoids in Eriocaulaceae Species and Their Antimicrobial Activity

Abstract

Quantification of prepared samples by analysis using high performance liquid chromatography with DAD detection was developed to analyze rutin, 6-methoxyapigenin, and 6-methoxyapigenin-7-O-β-D-glucopyranoside isolated from methanolic extracts of different genus: Syngonanthus, Leiothix and Eriocaulon (Eriocaulaceae). The linearity, accuracy, and the inter-day precision of the procedure were evaluated. The calibration curves were linear. The recoveries of the flavonoids in the samples analyzed were 96.3% to 98.5%. The percentage coefficient of variation for the quantitative analysis of the flavonoids in the analyses of the samples was under 5%. The antimicrobial activity of the five methanol extracts of these Eriocaulaceae species was assayed against the microorganisms Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella setubal, Saccharomyces cerevisiae and Candida albicans. Measured MIC values ranged from 1.25 to 10.00 mg/mL. The flavonoid contents suggest that Eriocaulaceae species may be a promising source of compounds to produce natural phytomedicines.

Chemical Constituents of the Methanolic Extract of Leaves of Leiothrix spiralis Ruhland and Their Antimicrobial Activity

“…The MIC values observed by us are similar to those reported by Silva et al . [ 32 ]; however, the activity was observed in the present study against other Candida species.…”

“…Silva et al . [ 32 ] have reported the anti- C. albicans activity of this extract. The MIC values observed by us are similar to those reported by Silva et al .…”

Correlation among Antioxidant, Antimicrobial, Hemolytic, and Antiproliferative Properties of Leiothrix spiralis Leaves Extract

“…Some species of this family are of great economic importance since they are exported as ornamental plants to various countries, mainly Germany and Japan [ 11 ]. Chemical studies of the contents of soluble phenolic compounds of Leiothrix species were performed and the presence of xanthones and flavones, including nepetin-7- O -β- d -glucopyranoside, nepetin-7- O -β- d -arabinopyranoside and luteolin O- and C- glucopyranoside, rutin, 6-methoxyapigenin-7- O -β - d - glucopyranoside and 6-methoxyapigenin, were identified [ 5 , 12 – 14 ]. Antimicrobial activity of phenolic compounds from Leiothrix spiralis was investigated, showing activity on different strains of bacteria and yeasts [ 15 ].…”

Phenolic compounds and antioxidant, antimicrobial and antimycobacterial activities of Serjania erecta Radlk. (Sapindaceae)

“…The antimicrobial activity of the ethanolic extracts was assayed using the broth microdilution method (Silva et al, 2009) , and the standard antibiotics chloramphenicol and nystatin were used in order to control the sensitivity of the microbial test. The minimal inhibitory concentration (MIC) was determined in 96-well culture plates by a microdilution method using a microorganism suspension at a density of 10 5 colonyforming units/mL with soy casein broth incubated for 24 hours at 37 °C for bacteria and Sabouraud broth incubated for 72 hours at 25 °C for yeasts.…”