High-performance liquid chromatographic method for the determination of pioglitazone in human plasma using ultraviolet detection and its application to a pharmacokinetic study

Sripalakit, Pattana; Neamhom, Penporn; Saraphanchotiwitthaya, Aurasorn

doi:10.1016/j.jchromb.2006.05.032

Cited by 69 publications

(42 citation statements)

References 10 publications

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“…Although in the range of concentrations used in most in-vitro studies establishing their role in cellular metabolism [29,38,39,42], we have used here drug concentrations well above plasma circulating concentrations of treated patients. For instance, the concentration for 1 h incubation with thiazolidinediones and 4 h with metformin corresponds approximately to a 10-to 15-fold higher exposure (concentration×time) than in patients in one day having the usual treatment [43,44].…”

Section: Discussionmentioning

confidence: 99%

Biguanides and thiazolidinediones inhibit stimulated lipolysis in human adipocytes through activation of AMP-activated protein kinase

et al. 2009

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Aims/hypothesis In rodent adipocytes, activated AMPactivated protein kinase reduces the lipolytic rate. As the hypoglycaemic drugs metformin and thiazolidinediones activate this enzyme in rodents, we tested the hypothesis that in addition to their known actions they could have an anti-lipolytic effect in human adipocytes. Methods Adipose tissue was obtained from individuals undergoing plastic surgery. Adipocytes were isolated and incubated with lipolytic agents (isoprenaline, atrial natriuretic peptide) and biguanides or thiazolidinediones. Lipolysis was quantified by the glycerol released in the medium. AMPactivated protein kinase activity and phosphorylation state were determined using standard procedures. Results In human adipocytes, isoprenaline and atrial natriuretic peptide stimulated the lipolytic rate three-to fourfold. Biguanides and thiazolidinediones activated AMPactivated protein kinase and inhibited lipolysis by 30-40%, at least in part by inhibiting hormone-sensitive lipase translocation to the lipid droplet. Inhibition of AMPactivated protein kinase by compound C precluded this inhibitory effect on lipolysis. Stimulation of lipolysis also induced an activation of AMP-activated protein kinase concomitant with a drop in ATP concentration. Conclusions/interpretation We show for the first time in human adipocytes that biguanides and thiazolidinediones activate AMP-activated protein kinase, thus counteracting lipolysis induced by lipolytic agents. In addition, β-agonistor ANP-stimulated lipolysis increases AMP-activated protein kinase activity. This is because of an increase in the AMP/ATP ratio, linked to activation of some of the released fatty acids into acyl-CoA. AMP-activated protein kinase activation could represent a physiological means of avoiding a deleterious drain of energy during lipolysis but could be used to restrain pharmacological release of fatty acids.

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Section: Discussionmentioning

confidence: 99%

Biguanides and thiazolidinediones inhibit stimulated lipolysis in human adipocytes through activation of AMP-activated protein kinase

et al. 2009

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“…Literature survey revealed HPLC, RP-HPLC, LC-MS, Spectrofluorimetric and simultaneous UV spectrophotometric methods are reported for the estimation of metformin hydrochloride [2][3][4][5], pioglitazone hydrochloride [6][7][8][9] and glibenclamide [10] alone or in combination with other anti-diabetic agents. So the present study aim to develop a simple, selective and precise RP-HPLC method for the simultaneous estimation of MET, PIO and GLI in bulk drug samples and in combined dosage formulation.…”

Section: Introductionmentioning

confidence: 99%

Stability Indicating RP-HPLC Method for the Simultaneous Estimation of Metformin Hydrochloride, Pioglitazone Hydrochloride and Glibenclamide in Bulk and Pharmaceutical Dosage Forms

S¹

2014

IOSRJPBS

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“…Pioglitazone decreases insulin resistance in the periphery and liver, resulting in increased insulindependent glucose disposal and decreased hepatic glucose output. Few methods for the determination of pioglitazone hydrochloride were reported including voltammetry in tablets and biological fluids [3], potentiometry in tablets [4], extractive spectrophotometry in raw material and tablets [5], HPLC in pharmaceutical formulations [6][7][8][9][10][11] and in biological fluids [11][12][13][14]. Pioglitazone hydrochloride has been determined in combination with other drugs using UV-spectrophotometry [15][16][17], HPLC [15,[18][19][20][21] in combined dosage forms, HPLC in formulations and human serum [22][23][24][25] and LC-MS methods in human…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of Stability Indicating HPLC Method for Quality Control of Pioglitazone Hydrochloride

2014

Can Chem Trans

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A simple, rapid and sensitive RP-HPLC method for the quantification of pioglitazone hydrochloride in bulk drug and tablet formulation was developed and validated. Chlordiazepoxide was used as internal standard. The separation was achieved on a Nucleodur 100-5 C18 column (250 mm× 4.6 mm i.d., 5 m particle size). The mobile phase consisted of methanol-0.07 M formic acid (57:43, v/v) at a flow rate of 0.9 mL/min and detection was performed at 266 nm using photodiode array (PDA) detector. The drug was subjected to various ICH prescribed stress conditions including hydrolysis (acid and alkaline), oxidation, photolysis and thermal degradation. Degradation in acid, base and peroxide was found in range 36-45%. The proposed method was validated with respect to specificity, linearity, accuracy, precision, limit of detection (LOD), limit of quantitation (LOQ), stability, and robustness as per ICH guideline. The developed method was found to be successively applied for the quality control of pioglitazone hydrochloride in bulk drug and tablets as well as the stability indicating studies.

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High-performance liquid chromatographic method for the determination of pioglitazone in human plasma using ultraviolet detection and its application to a pharmacokinetic study

Cited by 69 publications

References 10 publications

Biguanides and thiazolidinediones inhibit stimulated lipolysis in human adipocytes through activation of AMP-activated protein kinase

Biguanides and thiazolidinediones inhibit stimulated lipolysis in human adipocytes through activation of AMP-activated protein kinase

Stability Indicating RP-HPLC Method for the Simultaneous Estimation of Metformin Hydrochloride, Pioglitazone Hydrochloride and Glibenclamide in Bulk and Pharmaceutical Dosage Forms

Development and Validation of Stability Indicating HPLC Method for Quality Control of Pioglitazone Hydrochloride

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