Stability Indicating RP-HPLC Method for the Simultaneous Estimation of Metformin Hydrochloride, Pioglitazone Hydrochloride and Glibenclamide in Bulk and Pharmaceutical Dosage Forms

S, SaiThanuja V Chandan R

doi:10.9790/3008-0914124133

Cited by 4 publications

(1 citation statement)

References 8 publications

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“…It is official in Indian Pharmacopoeia, pairing HPLC, 15 Stability indicating HPTLC, 16,17 Stability indicating PHPLC, [18][19][20][21][22][23][24] LCMS. Benfotiamine (S-benzoylthiamine O-monophosphate) is a member of allithiamines group which is a synthetic S-acyl derivative of thiamine (vitamin-B1).…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of Stability Indicating HPTLC Method for the Determination of Metformin Hydrochloride and Benfotiamine in Bulk and Combined Dosage Form

Sakhare¹,

Pekamwar²,

Mohkare³

2017

IJPER

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Context: A Simple, selective, precise, and Stability indicating High Performance Thin Layer Chromatography (HPTLC) method of analysis of Metformin Hydrochloride (MET) and Benfotiamine (BENT) both as a bulk drug and in their combined formulation has been developed. Method: The basic aim of this method is to separate both the drugs by HPTLC and measure their spots at 249 nm. The separation was carried out on TLC aluminium sheets of silica gel 60F 254 using Benzene: Methanol: Triethylamine (8.5:1:0.5, v/v/v) as a mobile phase. Stability of MET and BENT was carried out by forced degradation study. Result: MET and BENT gave distinct and well defined peak at Rf 0.26 and 0.72, respectively. Calibration curves were linear in range of 500-3000 and 75-450 ng/spot for MET and BENT, respectively. Method was successively applied to tablet formulation. Stability study shows that the chromatograms of samples degraded with acid, base, hydrogen peroxide, light and dry heat showed well separated spots of pure MET and BENT as well as some additional peaks at different RF values. Conclusion: The HPTLC method was also able to selectively quantitate Metformin hydrochloride and Benfotiamine in presence of their degradation products obtained in forced degradation study. Hence, the method can be used as stability indicating. The method was validated as per ICH guidelines and it is applied for the analysis of pharmaceutical dosage form containing these two drugs.

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Section: Introductionmentioning

confidence: 99%

Development and Validation of Stability Indicating HPTLC Method for the Determination of Metformin Hydrochloride and Benfotiamine in Bulk and Combined Dosage Form

Sakhare¹,

Pekamwar²,

Mohkare³

2017

IJPER

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Thiazolidinediones: Recent Development in Analytical Methodologies

Patel

2023

Journal of Chromatographic Science

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The instrumental analytical methods that have been developed and utilized for the determination of thiazolidinedione in bulk medications, formulations and biological fluids have been reviewed after an in-depth analysis of the literature published in a variety of analytical and pharmaceutical chemistry-related journals. The approaches covered by this research, which covers the years 2001–2022, include complex methods for analysis, chromatographic techniques and spectrometric analytical procedures. The mobile phase, flow rate, sample matrix, wavelength and other factors identified in the literature were just a few of the parameters used to evaluate thiazolidinediones. The present review focuses on the published analytical techniques for thiazolidinedione analysis that have been previously identified in the literature. The specified outcomes followed extensive learning, and the most recent advances in analytical methods for the identification of pioglitazone, pioglitazone HCl, rosiglitazone, rosiglitazone maleate and lobeglitazone were reviewed. Additionally, this article briefly discusses features of analytical discovery on thiazolidinediones, which will enable readers to access all discoveries in one place with precise outcomes.

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A Sensitive HPTLC Method for the Estimation of Glibenclamide, Rosiglitazone Maleate and Metformin Hydrochloride from a Multicomponent Dosage Form

Bhende

Varanasi²,

Abbulu

2020

Journal of Chromatographic Science

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A sensitive, rapid and cost-effective method based on HPTLC with UV detection was developed for the quantitation of Glibenclamide (GLIBEN), Rosiglitazone maleate (ROSI) and Metformin hydrochloride (MET) from a combined dosage form. Pre-coated RP-18 F254s aluminum sheets were used as the stationary phase. Methanol–tetrahydrofuran–water–glacial acetic acid (16: 3.6: 4: 0.4, v/v) used as the mobile phase, along with chamber saturation of 10 min offered an optimum migration (Rf = 0.54, 0.62 and 0.80 for GLIBEN, ROSI and MET, respectively). TLC Scanner 3 was used for densitometric evaluation of the chromatograms. DigiStore 2 Documentation System with winCATS software version 1.4.10 was used for the quantitation and photodocumentation. The LOD for GLIBEN, ROSI and MET was found to be 80 ng, 80 ng and 48 ng, respectively. Moreover, the LOQ was 200 ng, 200 ng and 120 ng for GLIBEN, ROSI and MET, respectively. The method was linear for GLIBEN (r = 0.9991), ROSI (r = 0.9993) and MET (r = 0.9988) within the tested range (200–1000, 200–1000 and 120–600 ng/band, respectively). The method was found to be precise and accurate for all the three drugs. The method was applied for the analysis of Triglucored tablets, and it proved to be a reliable quality control tool for the routine analysis of GLIBEN, ROSI and MET in a combined dosage form.

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Stability Indicating RP-HPLC Method for the Simultaneous Estimation of Metformin Hydrochloride, Pioglitazone Hydrochloride and Glibenclamide in Bulk and Pharmaceutical Dosage Forms

Cited by 4 publications

References 8 publications

Development and Validation of Stability Indicating HPTLC Method for the Determination of Metformin Hydrochloride and Benfotiamine in Bulk and Combined Dosage Form

Development and Validation of Stability Indicating HPTLC Method for the Determination of Metformin Hydrochloride and Benfotiamine in Bulk and Combined Dosage Form

Thiazolidinediones: Recent Development in Analytical Methodologies

A Sensitive HPTLC Method for the Estimation of Glibenclamide, Rosiglitazone Maleate and Metformin Hydrochloride from a Multicomponent Dosage Form

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