High Performance Liquid Chromatographic Determination of Diclofenac Sodium in Plasma Using Column‐switching Technique for Sample Clean‐up

Abstract

For routine analysis of diclofenac sodium in plasma, a new high performance liquid chromatographic method, which is combined with column-switching technique is developed. The precolumn packed with Corasil RP C-18 was connected to analytical column by switching system in order to enrich the sample drugs in plasma without extraction. This method showed excellent sensitivity, precision and reproducibility. The limit of detection, using a 100 microL injection of plasma, was 0.1 micrograms/mL and the mean coefficient of variation for intra- and inter-assay was better than 4.6%. Total analysis time was 20 min between injections. The present method offers distinct practical advantages over conventional liquid-liquid extraction methods of sample preparation with respect to time, effort, recovery, and sample volume required. The method has been applied to the samples from rats receiving oral administration of diclofenac sodium.

In-vitro protein binding interaction between a metabolite of triflusal, 2-hydroxy-4-trifluoromethylbenzoic acid and other drugs

“…The concentration of free (fu) HTB in serum was determined by HPLC (Ramis et al 1990). The f, values of glisentide, diclofenac, ibuprofen, indomethacin, naproxen, piroxicam and salicylic acid were determined by HPLC (Applied Biosystems Inc., Foster City, CA, USA) in accordance with the chromatographic conditions described by other authors (Emilsson et a1 1986;Lapicque et al 1989;Lee et al 1989;Streete 1989). The radioactivity of the ultrafiltrate with [I4C]warfarin was measured in an LS-3800 scintillation counter (Beckman Instruments Inc., Fullerton, CA, USA) after adding 10 mL Optiphase Hisafe I1 scintillation liquid (LKB Scintillation Products, UK) to each sample.…”

Effect of Altitude (2500 M) on The Pharmacokinetics of Diclofenac Sodium and Cefadroxil After Oral Administration To Human Volunteers

“…The present study describes a highly sensitive, accurate, and reproducible HPLC method for the determination of DF in human plasma. This method has several advantages over the previously reported methods [41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57]. Most of the published methods do not include an internal standard (IS), which is crucial because the sample preparation methods involve more than one extraction step [41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57].…”

“…This method has several advantages over the previously reported methods [41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57]. Most of the published methods do not include an internal standard (IS), which is crucial because the sample preparation methods involve more than one extraction step [41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57]. Sample preparation is simpler, the chromatographic column and internal standard used are available commercially.…”

“…[38][39][40]. There are large number of studies on determination of diclofenac sodium [40][41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56].…”

Subcutaneous concentrations following topical iontophoretic delivery of diclofenac

“…Several methods have been reported to analyze diclofenac levels in the biological fluids (17)(18)(19)(20)(21)(22)(23)(24)(25). The operating parameters were adopted from literature and the simplified HPLC assay was validated for intraday and inter-day variations.…”