High-performance liquid chromatographic determination of urinary free catecholamines with electrochemical detection after prepurification on immobilized boric acid

Speek, A.J.; Odink, J.; Schrijver, J.; Schreurs, W.H.P.

doi:10.1016/0009-8981(83)90060-8

Cited by 32 publications

(4 citation statements)

References 17 publications

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“…One milliliter of this was put into autosampler vials and mixed with 20 μl 5 M HCl. For injection of the eluate into the HPLC system the flow rate was adjusted to 0.8 ml/m and the detection range of the electrochemical detector was set at 610 mV (Intra-assay -precision <5%, inter-assay-precision <6%) [19,20].…”

Section: Methodsmentioning

confidence: 99%

Postmortem determination of concentrations of stress hormones in various body fluids—is there a dependency between adrenaline/noradrenaline quotient, cause of death and agony time?

Wilke¹,

Janssen²,

Fahrenhorst

et al. 2007

Int J Legal Med

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To find out whether a certain cause of death or a certain length of an agonal period shows specific adrenaline or noradrenaline profiles, heart blood, femoral vein blood, liquor, urine and vitreous humour were taken from corpses (n = 98) at the Medical School Hannover, and noradrenaline and adrenaline were determined using high-performance liquid chromatography (HPLC). Corpses were classified according to the following five categories: short agony, long agony, state after hanging, state after asphyxiation and state after CPR with documented administration of epinephrine. Once results were collected the adrenaline/noradrenaline quotient was determined. It became clear that there were no significant differences regarding the concentration of adrenaline and noradrenaline in the various body fluids in relation to the above-mentioned categories. The means adrenaline/noradrenaline quotients in femoral vein blood were 0.21 +/- 0.29 for hanged persons, 0.38 +/- 0.47 for asphyxiated persons, 0.17 +/- 0.19 for those with short agony and 0.42 +/- 0.43 for those with long agony, significantly below 1 (p < 0.001; p = 0.001; p = 0.003). For condition after CPR we found an adrenaline/noradrenaline quotient of 2.81 +/- 5.8. In liquor the adrenaline/noradrenaline quotients for short agony was 0.17 +/- 0.17, for hanged persons 0.18 +/- 0.19 and for asphyxiated ones 0.30 +/- 0.38, significantly lower than 1 (p < 0.001). In urine the adrenaline/noradrenaline quotients for all categories are lower than 1 (p < 0.001); short agony (0.13 +/- 0.09), long agony (0.21 +/- 0.16), hanged (0.15 +/- 0.16), asphyxiated (0.14 +/- 0.08) and CPR (0.14 +/- 0.06). In vitreous humour the quotients for short agony (0.14 +/- 0.28), long agony (0.13 +/- 0.12), hanged (0.07 +/- 0.09) and asphyxiated (0.09 +/- 0.11) are lower than 1 (p < 0.001). The spread of data for the adrenaline/noradrenaline quotient did not allow for any conclusions about cause of death and length of agony in individual cases.

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Section: Methodsmentioning

confidence: 99%

Postmortem determination of concentrations of stress hormones in various body fluids—is there a dependency between adrenaline/noradrenaline quotient, cause of death and agony time?

Wilke¹,

Janssen²,

Fahrenhorst

et al. 2007

Int J Legal Med

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“…Since the occurrence of nucleosides in physiological fluids turned out to be predicative for the diagnosis of pathobiochemical processes, the on-line System is the method of choice, äs it is particularly suitable for automatization and applicable for routine clinical analysis. As affinity chromatography on boronic acid-functionalized polymers is also well known for the pre-purification of catecholamines in physiological fluids (34)(35)(36)(37)(38), for the Separation of ribonucleosides and ribonucleotides from the corresponding deoxy-compounds (22,39,40), the Separation of RNA from DNA (41,42) and the Separation of queuine-containing tRNA from non-queuine tRNA (41) or aminoacyl-tRNA from uncharged tRNA (41,42) the use of boronate HPLAC in combination with the presented column switching technique offers a wide ränge of application.…”

Section: Discussionmentioning

confidence: 99%

Development of a Chromatographic Method for the Quantitative Determination of Minor Ribonucleosides in Physiological Fluids. Characterization and quantitative determination of minor ribonucleosides in physiological fluids, Part I

Hagemeier¹,

Kemper²,

Ks³

et al. 1984

Clinical Chemistry and Laboratory Medicine

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The authors wish to congratulate Professor Walther Lamprecht, Hannover, on the occasion of his sixtieth birthday Summary:We describe an on-line multi-cplumn high performance liquid Chromatographie method for the selective clean-up and analysis of major and minor ribonucleosides in physiological fluids. Quantitative data obtained for the determination of some methylated ribonucleosides in human urines are compared with those obtained with the traditional off-line method.The on-line technique developed in our laboratory is distinguished from the off-line method by the following features:(1) Sample clean-up and analysis of the target-compounds can easily be automatized, (2) Total time of analysis, for example of urinary ribonucleosides, is decreased to 35 minutes, (3) Laborious and error-prone evaporation and redissolution Steps are avoided, (4) Reliability of the overall analytical System can be controlled with ease, (5) Small sample-volumes can be applied directly, (6) Sensitive samples can be processed very rapidly under mild conditions, (7) Results obtained with the -line and off-line-techniques compare well. Hagemeier, Kemper, Boos and Schlimme: Quantitative determination of minor ribonucleosides (5) Kleine Probenvolumina können direkt appliziert werden, (6) Empfindliche Proben können in kürzester Zeit unter schonenden Bedingungen analysiert werden, (7) Die mit der "on-line" wie mit der "off-line"-Technik erhaltenen Ergebnisse zeigen eine gute ÜbereinŜ timmung. Entwicklung einer chromatographischen Methode zur quantitativen Bestimmung seltener Ribonucleoside in physiologischen Flüssigkeiten Charakterisierung und quantitative Bestimmung von seltenen Ribonucleosiden in physiologischen Flüssig

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“…Various analytical techniques for CAs analysis have been reported. Reverse-phase liquid chromatography (RPLC) using C18 columns hyphenated to different detection systems, including mass spectrometry (MS) [21][22][23], electrochemical detection (ECD) [24][25][26][27][28], and fluorescence detection [29] has been widely applied. However, due to the non-polar character of C18 phases and the high polarity of the CAs, derivatization steps, as well as ion pair additives are often considered.…”

Section: Introductionmentioning

confidence: 99%

Efficient Sub-1 Minute Analysis of Selected Biomarker Catecholamines by Core-Shell Hydrophilic Interaction Liquid Chromatography (HILIC) with Nanomolar Detection at a Boron-Doped Diamond (BDD) Electrode

Alsaeedi¹,

Alghamdi²,

Hayes³

et al. 2021

Separations

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A rapid, sensitive method for the separation of catecholamine biomarkers (CAs), of importance in traumatic brain injury (TBI) and in Parkinson’s disease (PD), has been successfully developed using hydrophilic interaction liquid chromatography (HILIC). Dopamine (DA), epinephrine (EPI), and norepinephrine (NE) are known to be three to fivefold elevated above normal in traumatic brain injury (TBI) patients. HILIC facilitates the rapid and efficient separation of these polar biomarkers, which can be poorly retained by reversed-phase liquid chromatography (RPLC), while electrochemical detection (ECD) at the boron-doped diamond (BDD) electrode provides enhanced nanomolar detection. Three HILIC columns were compared, namely the superficially porous (core-shell) Z-HILIC column and the Z-cHILIC and Z-HILIC fully porous columns. The core-shell Z-HILIC showed the highest efficiency with a rapid separation within 60 s. The HILIC method utilizing the core-shell Z-HILIC column was initially optimized for the simultaneous analysis of DA, EPI, and NE using UV detection. The advantages of using the BDD electrode over UV detection were explored, and the improved limits of detection (LODs, S/N = 3) measured were 40, 50, and 50 nM for DA, EPI, and NE, respectively. Method validation is reported in terms of the linearity, repeatability, reproducibility, and LODs. Furthermore, the proposed method was successfully applied to the real sample analysis of urinary CAs following phenylboronic acid (PBA) solid phase extraction (SPE) pretreatment.

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High-performance liquid chromatographic determination of urinary free catecholamines with electrochemical detection after prepurification on immobilized boric acid

Cited by 32 publications

References 17 publications

Postmortem determination of concentrations of stress hormones in various body fluids—is there a dependency between adrenaline/noradrenaline quotient, cause of death and agony time?

Postmortem determination of concentrations of stress hormones in various body fluids—is there a dependency between adrenaline/noradrenaline quotient, cause of death and agony time?

Development of a Chromatographic Method for the Quantitative Determination of Minor Ribonucleosides in Physiological Fluids. Characterization and quantitative determination of minor ribonucleosides in physiological fluids, Part I

Efficient Sub-1 Minute Analysis of Selected Biomarker Catecholamines by Core-Shell Hydrophilic Interaction Liquid Chromatography (HILIC) with Nanomolar Detection at a Boron-Doped Diamond (BDD) Electrode

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