High-performance liquid chromatographic determination of valproic acid in plasma using a micelle-mediated pre-column derivatization

Headspace Solid-Phase Microextraction-Gas Chromatography Method for the Determination of Valproic Acid in Human Serum, and Formulations Using Hollow-Fiber Coated Wire

“…Gas chromatography (GC), [3][4][5][6][7][8][9] liquid chromatography [10][11][12][13][14][15][16][17][18][19][20] and capillary electrophoresis [21][22][23] are the main reported techniques for the determination of VPA.…”

Determination of valproic acid in human serum and pharmaceutical preparations by headspace liquid-phase microextraction gas chromatography-flame ionization detection without prior derivatization

“…Many analytical approaches have been used in determination of VPA, based on high-performance liquid chromatography (HPLC) [3][4][5][6][7][8][9], capillary electrophoresis [10,11] in combination with mass spectrometry (MS) [12][13][14][15][16][17][18], ultraviolet detection (UV) or fluorescence detection, usually after derivatization with a suitable chromophore or fluorophore [19][20][21], and also potentiometry ion selective sensors [22,23]. Although HPLC methods are frequently applied for this analysis but due to volatile nature of VPA, GC-MS is often preferred, offering unrivalled high resolution [24][25][26][27].…”

“…Extraction with solvent is most commonly used for isolation of VPA of serum solution prior to chromatographic analysis [3][4][5][6][7][8][9][10][11][12][19][20][21]29]. Evaporation and clean-up of extractive solvent prior to injection to GC column is essential to concentrate and eliminate background interferences.…”

Simultaneous determination of mycophenolic acid and valproic acid based on derivatization by high‐performance liquid chromatography with fluorescence detection

“…To date, a number of HPLC methods have been developed to determine MPA (Huang et al, 1997;Jones et al, 1998;Yau et al, 2004;Shipkova et al, 1998) and VPA (Alric et al, 1981;Chen et al, 2000Chen et al, , 2001; Van der Horst et al, 1988;Lin et al, 2004) in plasma. However, none of them can quantify both MPA and VPA in a single HPLC run.…”