High-performance liquid chromatographic determination of tentoxin in fermentation of Alternaria porri (Ellis) Ciferri

Development of a Stable Isotope Dilution LC–MS/MS Method for the Alternaria Toxins Tentoxin, Dihydrotentoxin, and Isotentoxin

“…For analysis of these metabolites, an LC–UV method for TEN was described by Suemitsu et al An LC–MS method using external calibration was published by Horiuchi et al, 2003, for simultaneous identification of TEN, DHT, and isoTEN from the culture liquid. Most recently, a semiquantitative analysis of TEN in moldy food samples via LC–MS/MS within their multimycotoxin screening without cleanup was developed by Sulyok et al…”

“…Therefore, the European Food and Safety Authority (EFSA) applied for its preliminary risk assessment the concept of thresholds of toxicological concern (TTC) for TEN, which was set to 1500 ng/kg body weight per day. 8 For analysis of these metabolites, an LC−UV method for TEN was described by Suemitsu et al 9 An LC−MS method using external calibration was published by Horiuchi et al, 2003, 10 for simultaneous identification of TEN, DHT, and isoTEN from the culture liquid. Most recently, a semiquantitative analysis of TEN in moldy food samples via LC− MS/MS within their multimycotoxin screening without cleanup was developed by Sulyok et al 11 Until now, there has been very limited analytical data of those toxins in food.…”

Detection of Tentoxin byAlternaria solani, A. cucumerina, A. bataticolaandA. japonica

“…7 ) This paper deals with the HPLC determination of tentoxin from a culture liquid of A. porri, A. solani, A. cucumerina, A. bataticola, and A. japonica, the causal fungus of plant disease .in stone-leek, potato, cucumber, sweet-potato and radish, respectively. These were grown as artificial cultures, i.e., Richards and Czapek Dox (with ZnS0 4 ), and natural cultures with potato sucrose medium.…”