Analytical Methods for the Determination of Alternaria Mycotoxins

Man, Yan; Liang, Gang; Liu, An; Pan, Ligang

doi:10.1007/s10337-016-3186-x

Cited by 38 publications

(23 citation statements)

References 94 publications

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“…Although the statutory or guideline limits have not been set for AME toxin in food and agricultural products by the regulatory authorities, recently the European Standing Committee suggested that EU member states should collect the occurrence data of Alternaria mycotoxins containing AME toxin in food. In addition, the European Food Safety Authority (EFSA) has published its scientific opinion about the health risk of AME toxin in food and feed [ 2 ]. So, the problem of food safety about AME toxin has caused wide public concern over the recent years.…”

Section: Discussionmentioning

confidence: 99%

“…Alternariol monomethyl ether (AME, 3, 7-Dihydroxy-9-methoxy-1-methyl-6H-dibenzo [b, d] pyran-6-one) is one of the major Alternaria mycotoxins. AME is structurally related to alternariol (AOH) and altenuene (ALT), and belongs to dibenzo-α-pyrone derivatives which are produced by Alternaria alternata , Alternaria solani and some other species within the genus Alternaria [ 1 , 2 ]. AME is distributed all over the world and shows no acute toxic effects to the health of humans and animals [ 3 ], but it possesses the properties of mutagenicity and carcinogenicity, causing particularly high incidences of esophageal cancer [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

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Development of an Immunochromatographic Strip Test for the Rapid Detection of Alternariol Monomethyl Ether in Fruit

Man

Liang

Jia

et al. 2017

Toxins

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A rapid, portable, and semi-quantitative immunochromatographic strip was developed for rapid and visual detection of alternariol monomethyl ether (AME). For this purpose, the anti-AME monoclonal antibody (mAb) was prepared and identified. AME coupled to bovine serum albumin (BSA) via methyl 4-bromobutanoate was prepared as immunogen. The recoveries of AME in spiked cherry and orange fruits determined by competitive ELISA were 86.1% and 80.7%, respectively. A colloidal gold nanoparticle (CGN) and CGNs-mAb conjugate were synthesized, and on this basis, a competitive colloidal gold immunochromatographic strip was developed and applied to the detection of AME toxin in fruit samples. The intensity of red density of the test line (T line) is inversely proportional to AME concentration in the range 0.1–10 ng/mL. The visual limit of detection (LOD) of AME was found to be about 10 ng/mL. The semi-quantitative detection can be completed in 10 min. Moreover, the immunochromatographic strip has lower cross-reactivity with AME analogues, and it has a good stability performance (following 3 months of storage). Hence, the colloidal gold immunochromatographic strip could be used as a semi-quantitative tool for the on-site, rapid, and visual detection of AME in fruit.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of an Immunochromatographic Strip Test for the Rapid Detection of Alternariol Monomethyl Ether in Fruit

Man

Liang

Jia

et al. 2017

Toxins

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“…Moreover, this compound is characterized by a poor peak shape in HPLC due to its high acidity at the prevailing pH (pKa 4.5) and metal chelation. This compound is normally not included in multi-residue methods as a pre-column derivatisation has been shown as requisite to enhance its sensitivity (Man, Liang, Li, & Pan, 2017). For both compounds another sample treatment and/or a more sensitive instrument might be required .…”

Section: Screening Detection Limitmentioning

confidence: 99%

Ultra-high-performance liquid chromatography coupled to quadrupole orbitrap high-resolution mass spectrometry for multi-residue screening of pesticides, (veterinary) drugs and mycotoxins in edible insects

Paepe

Wauters

Borght³

et al. 2019

Food Chemistry

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A generic extraction and UHPLC-Q-Orbitrap TM-HRMS method was developed for four insect species (mealworm, grasshopper, house cricket and black soldier fly) analyzing a large spectrum of organic chemical contaminants, including pesticides (n=25), (veterinary) drugs (n=29), and mycotoxins (n=23). To prove the method as 'fit-for-purpose', a successful validation was performed, both qualitatively, by determining the screening detection limit (SDL), selectivity and specificity, as well as semi-quantitatively, by assessing the within-day precision (relative standard deviation (RSD)) and recovery. For both the mealworm, grasshopper, house cricket and black soldier fly, 64, 61, 59 and 62 compounds were detected at the respective SDL levels (1-100 g kg-1), predominantly below existing maximum residue limits for other edible matrices. Mean recoveries ranged between 70% and 120% and RSD-values were in line with European regulations (CD 2002/657/EC; SANCO). Finally, the potential of the screening methodology was demonstrated on real insect samples, revealing minor to no contamination.

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“…Instead, proper sample preparation for toxins extraction and quantification by MRM using triple quads is the most common MS-based methodology applied [66][67]. Nuts [68], maize [69], shellfish [70], tomato [71], or beer [72], among others, are examples of food products assayed following this approach.…”

Section: Detection Of Microbial Contaminants (Pathogens and Toxins)mentioning

confidence: 99%

Application of mass spectrometry-based metabolomics approaches for food safety, quality and traceability

Castro-Puyana

Pérez-Míguez

Montero

et al. 2017

TrAC Trends in Analytical Chemistry

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The always more-demanding fields of food safety, quality and traceability are continuously fostering the development of robust, efficient, sensitive and cost-effective analytical methodologies. Mass spectrometry-based metabolomics is a key tool nowadays with great potential in many analytical fields and has been demonstrated to be capable of facing some important challenges related to these areas within the food science domain. The main aim of this review is to present a critical overview of the most recent applications of MS-based metabolomics approaches for food quality, safety and traceability assessment, covering the most relevant works published from 2014 to 2017. Information about the different steps needed to develop a MS-metabolomics approach, i.e. sample treatment, analytical platform, and data processing, is also provided and discussed.

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Analytical Methods for the Determination of Alternaria Mycotoxins

Cited by 38 publications

References 94 publications

Development of an Immunochromatographic Strip Test for the Rapid Detection of Alternariol Monomethyl Ether in Fruit

Development of an Immunochromatographic Strip Test for the Rapid Detection of Alternariol Monomethyl Ether in Fruit

Ultra-high-performance liquid chromatography coupled to quadrupole orbitrap high-resolution mass spectrometry for multi-residue screening of pesticides, (veterinary) drugs and mycotoxins in edible insects

Application of mass spectrometry-based metabolomics approaches for food safety, quality and traceability

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