Development of an Immunochromatographic Strip Test for the Rapid Detection of Alternariol Monomethyl Ether in Fruit

Man, Yan; Liang, Gang; Jia, Fuchao; Liu, An; Fu, Hailong; Wang, Meng; Pan, Ligang

doi:10.3390/toxins9050152

Cited by 18 publications

(10 citation statements)

References 27 publications

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“…The first antibodies and immunoassays for AOH were reported in 2011 [10,11]. Since then, a few immunoassays have been described using either polyclonal or monoclonal antibodies specific to AOH [12,13], and only one study has been published using an antibody specific to AME [14]. Additionally, Wang et al have reported a generic antibody for both mycotoxins [15].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Optimum Linker Tethering Site of Alternariol Haptens for Antibody Generation and Immunoassay Development

Addante-Moya

Abad‐Somovilla

Abad‐Fuentes

et al. 2021

Toxins

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Immunochemical methods for mycotoxin analysis require antigens with well-defined structures and antibodies with outstanding binding properties. Immunoreagents for the mycotoxins alternariol and/or alternariol monomethyl ether have typically been obtained with chemically uncharacterized haptens, and antigen conjugates have most likely been prepared with mixtures of functionalized molecules. For the first time, total synthesis was performed, in the present study, to obtain two haptens with opposite linker attachment locations. The functionalized synthetic haptens were purified and deeply characterized by different spectrometric methods, allowing the preparation of bioconjugates with unequivocal structures. Direct and indirect competitive enzyme-linked immunosorbent assays, using homologous and heterologous conjugates, were employed to extensively evaluate the generated immunoreagents. Antibodies with high affinity were raised from conjugates of both haptens, and a structure-activity relationship between the synthetic haptens and the specificity of the generated antibodies could be established. These results pave the way for the development of novel highly sensitive immunoassays selective of one or two of these Alternaria mycotoxins.

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Section: Introductionmentioning

confidence: 99%

Assessment of the Optimum Linker Tethering Site of Alternariol Haptens for Antibody Generation and Immunoassay Development

Addante-Moya

Abad‐Somovilla

Abad‐Fuentes

et al. 2021

Toxins

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“…The following reagents were used: PBS; boric acid buffer solution (0.2 mol/L); resuspension solution, gold label pad treatment solution, sample pad treatment solution, sample buffer [23].…”

Section: Reagents and Solutionsmentioning

confidence: 99%

Simultaneous Rapid Detection of Aflatoxin B1 and Ochratoxin A in Spices Using Lateral Flow Immuno-Chromatographic Assay

Zhao

Jin

Lin

et al. 2021

Foods

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Spices are susceptible to contamination by aflatoxin B1 (AFB1) and ochratoxin A (OTA), which are both mycotoxins with high toxicity and carcinogenicity. In this study, we aimed to develop an immuno-chromatographic strip test for the simultaneous quantification of AFB1 and OTA in spices by spraying the coupled antigens AFB1–ovalbumin (AFB1–OVA) and OTA–ovalbumin (OTA–OVA) on a nitrocellulose membrane. The test strip had high sensitivity, good specificity, and strong stability. The detection limits of these two mycotoxins in Chinese prickly ash, pepper, chili, cinnamon, and aniseed were 5 μg/kg. The false positivity rate was 2%, and the false negativity rate was 0%. The maximum coefficient of variation was 4.28% between batches and 5.72% within batches. The average recovery rates of AFB1 and OTA in spices were 81.2–113.7% and 82.2–118.6%, respectively, and the relative standard deviation (RSD) was <10%. The actual sample detection was consistent with high performance liquid chromatography analysis results. Therefore, the immuno-chromatographic test strips developed in this study can be used for the on-site simultaneous detection of AFB1 and OTA in spices. This method would allow the relevant regulatory agencies to strengthen supervision in an effort to reduce the possible human health hazards of such contaminated spices.

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“…However, in beverages, these methods have largely been disseminated for alcoholic drink (e.g., wine and grape must [211]), milk [212,213] and cereal analysis [214]. Recent reports have described the use of these techniques to assess Alternaria toxins in fruit [215] and fruit juice [216].…”

Section: Non-chromatographic Multiple Mycotoxin Analysismentioning

confidence: 99%

Mycotoxin Contamination of Beverages Obtained from Tropical Crops

2018

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This review is mainly centered on beverages obtained from tropical crops, including tea, nut milk, coffee, cocoa, and those prepared from fruits. After considering the epidemiological data found on the matrices above, the focus was given to recent methodological approaches to assess the most relevant mycotoxins. Aspects such as singularities among the mycotoxin and the beverage in which their were found, and the economic effects and repercussions that the mycotoxin-tainted ingredients have on the beverage industry were pointed out. Finally, the burden of their consumption through beverages, including risk and health effects on humans, was addressed as well.

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Development of an Immunochromatographic Strip Test for the Rapid Detection of Alternariol Monomethyl Ether in Fruit

Cited by 18 publications

References 27 publications

Assessment of the Optimum Linker Tethering Site of Alternariol Haptens for Antibody Generation and Immunoassay Development

Assessment of the Optimum Linker Tethering Site of Alternariol Haptens for Antibody Generation and Immunoassay Development

Simultaneous Rapid Detection of Aflatoxin B1 and Ochratoxin A in Spices Using Lateral Flow Immuno-Chromatographic Assay

Mycotoxin Contamination of Beverages Obtained from Tropical Crops

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