High–performance liquid chromatographic determination of nitrofurantoin in plasma

Abstract

SUMMARY A reversed–phase high–performance liquid chromatographic method for the analysis of nitrofurantoin in rabbit plasma was developed. Acetanilide was used as the internal standard. The chromatography was performed using a C–18 column; the mobile phase consisted of 75:25 water to methanol; a flow rate of 09 ml/min; and UV detection at 270 run. Retention times were 58 and 101 min for nitrofurantoin and acetanilide, respectively. Recovery of nitrofurantoin added to plasma in the concentration range of 005–5 μg/ml was found to be greater than 92%. This procedure was used for the analysis of plasma samples collected over time following the oral administration of nitrofurantoin tablets to rabbits.

Suppression of Adjuvant-Induced Arthritic Bone Destruction by Cyclooxygenase-2 Selective Agents With and Without Inhibitory Potency Against Carbonic Anhydrase II

“…(33)(34)(35) This range of dosage was also determined to cover their clinical daily dosages: 4 mg/kg for celecoxib, 0.5 mg/kg for rofecoxib, 2-5 mg/kg for JTE-522, and 5 mg/kg for acetazolamide. Although the dosage of rofecoxib seems about 1/10 of those of other drugs, gastrointestinal absorption of this drug in rats is reported to be about 1/10 that in humans, (36,37) suggesting that these drugs of the same dosage have similar biological potencies in AIA rats. Indeed, this does not lead to the conclusion that the efficacy of the agents can be compared simply by the ED 50 values calculated by the dose-response experiments (Fig.…”

Analytical Aspects of Amino, Quaternary Ammonium, Nitro, Nitroso and Related Functional Groups

“…260 and 261 were determined in shrimp muscle tissue by LC 541 . 263 was determined in plasma of rabbits by RP-HPLC, using acetanilide as internal standard 542 . LC was used to determine residues of 261 in chicken raised to maturity on a diet fortified with 0.0055% of this drug 543 .…”