High-performance liquid chromatographic determination of colouring matters in historical garments from the Holy Mountain of Athos

Extracting natural dyes from wool—an evaluation of extraction methods

“…Neither brazilin or brazilein were detected. Nevertheless, a compound with r t =18.80 min in the chromatographic profile of this sample extract is tentatively identified as Type C compound (Table 2), an analyte previously described by Karapanagiotis et al in samples collected from Cretan icons [42] and post-Byzantine textiles [43]. The authors [42,43] did not suggest an explanation for the nature of this compound.…”

“…Nevertheless, a compound with r t =18.80 min in the chromatographic profile of this sample extract is tentatively identified as Type C compound (Table 2), an analyte previously described by Karapanagiotis et al in samples collected from Cretan icons [42] and post-Byzantine textiles [43]. The authors [42,43] did not suggest an explanation for the nature of this compound. However, preliminary results obtained by our group on light artificially aged wool samples dyed with brazilwood and extracted by the Na 2 EDTA/DMF method suggest that Type C compound is likely to be a photodegradation product of this natural dye (unpublished data).…”

Investigation of the colourants used in icons of the Cretan School of iconography

“…Natural organic colouring materials used in objects of the cultural heritage are identified by electrochemical methods [18][19][20] and more commonly by high performance liquid chromatography (HPLC) equipped with diode array detector (DAD) [21][22][23][24][25][26][27][28][29][30][31] and/or mass spectrometry (MS) [32][33][34][35][36][37][38][39][40][41][42][43][44][45]; HPLC with fluorescence detection has been also used [46]. Prior to HPLC analysis, samples have to be treated to extract and solubilise the contained dyestuffs.…”

Rapana venosa da Bulunan İndigoid Boyarmaddelerin Yüksek Performanslı Sıvı Kromatografisi - Diyod-sıralı Dedektör (HPLCDAD) ile Belirlenmesi

“…Çalışma sonucunda geliştirilen kromatografik yöntemin indigoid boyarmaddelerin ayrılmasında literatürde önerilen yöntemlere göre daha başarılı olduğu tespit edilmiştir (4,13). Yöntem Rapana venosa numunesindeki maddelerin belirlenmesi içinde yeterli kromatografik ayırımı sağlamıştır.…”