High-performance liquid chromatographic determination of conjugated estrogens in tablets

Pharmacokinetics of Exogenous Natural and Synthetic Estrogens and Antiestrogens

“…Other methods described include a chemiluminescent immunoassay (SATO et al 1996); receptor-binding assays (IIDA et al 1991); high-performance liquid chromatography with photometric detection (CASTAGNETTA et al 1991;DESTA 1988;SUKKER et al 1981;TOWNSEND et al 1988); chemiluminescence detection (NOZAKI et al 1988); electrochemical detection (NOMA et al 1991) or ftuorimetric detection (KATAYAMA and TANIGUCHI 1993;CHANDRASEKARAN et al 1996); adsorptive stripping voltammetry (Hu et al 1992); recombinant cell bioassay (KLEIN et al 1994); and gas chromatography-mass spectrometry (GC-MS) (SPINK et al 1990;VANLUCHENE et a1.1983;ZIMMERMANN et al 1998b).…”

Pyrene Sulphonyl Chloride as a Reagent for Quantitation of Oestrogens in Human Serum Using HPLC with Conventional and Laser-Induced Fluorescence Detection

“…This method is, however, laborious and expensive. A number of HPLC techniques coupled with chemiluminescence, electrochemical and UV absorbance detection have been reported for oestrogen analysis (Nozaki er al., 1988;Noma, et al, 1991;Desta, 1988;Townsend et al, 1988;Castagnetta, et al, 1991). Although these methods are superior in simplicity, rapidity and safety, no HPLC method thus far reported has achieved adequate sensitivity to determine oestrogens at the low pg/mL concentrations that are necessary for clinical monitoring of oestrogen therapy.…”