High-performance liquid chromatographic assay for ibuprofen in whole blood using solid-phase extraction

Direct potentiometry and potentiotitrimetry of warfarin and ibuprofen in pharmaceutical preparations using PVC ferroin-based membrane sensors

“…The working range of fluorimetry [8,9] amperometry [10] and capillary electrophoresis [7] methods used for warfarin assay are 0.2-4 gg/ml, 1-40 gg/ml and 0.02-2gg/ml, respectively, compared with 6.6-300.0 gg/ml (lower detection limit 0.8 gg/ml) of the warfarin sensor. The working range offered by the present ibuprofen sensor (4-2000 gg/ml, lower detection limit 1.3 gg/ml) compares favourably with those obtained by spectrophotometry (10-3000 gg/ml) [18][19][20], high performance liquid chromatography (0.2-200 gg/ml) [11][12][13] and polarography (80-800 gg/ml) [17]. The overall assay time of the present method is less than 5 rain and the method does not involve any pretreatment, derivatization or extraction steps, as are necessary with other instrumental techniques in current use [6,[18][19][20][21].…”

“…[21]. Many of these methods, however, involve several manipulation, extraction and derivatization steps [6,[18][19][20][21], suffer from lack of sensitivity [3,4,20], and require sophisticated instrumentation [5][6][7][11][12][13][14][15][16]. The advantages offered by capillary electrophoresis and HPLC techniques are the detection of enantiomers and metabolites of the drugs.…”

“…Ibuprofen has been also determined by methods based on HPLC [11][12][13], differential scanning calorimetry [14], capillary zone electrophoresis [15,16], polarography [17], spectrophotometry [18][19][20] and spectrofluorimetry [21]. Many of these methods, however, involve several manipulation, extraction and derivatization steps [6,[18][19][20][21], suffer from lack of sensitivity [3,4,20], and require sophisticated instrumentation [5][6][7][11][12][13][14][15][16].…”

Liquid chromatographic analysis of certain commercial formulations for non‐opioid analgesics

“…Determination of ibuprofen in pharmaceutical and biological fluids has been reported using HPLC (Sochor et al, 1995;Sochor et al, 1994;Castillo and Smith, 1993;Bhushan et al, 2005) and gas-liquid chromatography (Vangiessan and Kaiser, 1975;Kaiser and Martin, 1978). Enantiomeric resolution of ibuprofen in pharmaceutical preparations has been reported (Bhushan and Gupta, 2004;Bhushan and Thiongo, 1999;Bhushan and Parshad, 1996).…”

Rp-HPLC Assay of Ibuprofen in Plasma Using Different Extraction Procedures

“…Like most of the non-steroidal antiinflammatory drugs, it is metabolized in the liver by oxidation and conjugation to inactive metabolites which are typically excreted in the urine. Various analytical procedures have been employed earlier for determination of IBF in body fluids, plasma and serum [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18] . However many of these assays comprise of complex extraction procedure and require more sample volume leading to difficulties in pharmacokinetic studies in small animals, neonates and children.…”