High-performance liquid chromatographic and capillary electrophoretic methods for the quantitation of the (S)-terbutaline in (R)-terbutaline

Abstract

KeyWordsColumn liquid chromatography Capillary electrophoresis Terbutaline Chiral separation Optical purity testing SummaryThe resolution of chiral drugs, metabolites and related substances continues to be an important area in pharmaceutical analysis. Two methods for the optical purity testing of (R)-(-)-terbutaline were developed, namely capillary electrophoresis using hydroxypropyl-13-cyclodextrin and high-performance liquid chromatography using a chiral stationary phase. Validation data such as linearity, recovery, detection limit, and precision of the ~o methods are presented. The detection limit of (S)-terbutaline in (R)-terbutaline was 0.05% by the HPLC method and 0.03% by the CE method. There was generally good agreement between the HPLC and CE results. These methods were bund to be applicable as a practical quality control method for the enantiomeric purity determination of (R)-terbutaline.

Terbutaline Enantiomer Separation and Quantification by Complexation and Field Asymmetric Ion Mobility Spectrometry−Tandem Mass Spectrometry

“…If this blank signal is due to contamination, the content of (−)-terbutaline in the used (+)-terbutaline is less than the calculated lod (i.e., <0.10%), and the enantiomeric excess (ee) of the (+)-terbutaline used was thus in excess of 99.80%. This result has been confirmed by CE measurements of the same batch of (+)-terbutaline following a method described elsewhere, which had a lod of 0.2% (−)-terbutaline of total terbutaline, and the (−)-terbutaline content was determined to be below this lod.…”

“…The target compound of this study was terbutaline, a β 2 -adrenergic receptor agonist used as a fast-acting bronchodilator (for short-term asthma treatment) and to delay premature labor . Terbutaline contains one chiral center and has been used as a test compound for investigation of many chiral separation techniques, particularly those using HPLC – and CE. – Comparative HPLC and CE methods for the quantitation of (+)-terbutaline in (−)-terbutaline have also been published . This allowed for the comparison of the new FAIMS-based separation method with a more conventional liquid-phase separation for enantiomers.…”

Separation and elution order of the enantiomers of some β-agonists using polysaccharide-based chiral columns and normal phase eluents by high-performance liquid chromatography

“…In literature separation of enantiomers of some chiral β-agonists are described using HPLC [14][15][16][17][18][19][20][21][22][23][24][25], supercritical fluid chromatography (SFC) [26], capillary electrophoresis (CE) [17,23,[27][28][29], capillary electrochromatography (CEC) [30] and micellar electrokinetic chromatography (MEKC) [31]. Some studies were devoted to investigation of enantiomer migration order and chiral recognition mechanisms in CE [17].…”

Quantification of Terbutaline in Pharmaceutical Formulation and Human Serum by Adsorptive Stripping Voltammetry at a Glassy Carbon Electrode

“…These include capillary electrophoresis, [5][6][7][8][9][10][11] flow-injection chemiluminescence, 12,13) colorimetry, [14][15][16][17] spectrophotometry, [18][19][20] liquid chromatography, [21][22][23][24] gas chromatography, 25) high performance liquid chromatography [26][27][28][29][30][31][32][33] and cyclic voltammetry. 34) Most of the reported methods require time-consuming extraction steps prior to the analysis.…”