Quantification of Terbutaline in Pharmaceutical Formulation and Human Serum by Adsorptive Stripping Voltammetry at a Glassy Carbon Electrode

Abstract

Terbutaline sulfate (Chart 1), 2-tert-butylamino-1-(3,5-dihydroxyphenyl) ethanol hemisulfate, is an adrenergic agonist which predominantly stimulates b 2 -receptor widely used in the treatment of bronchial asthma, chronic bronchitis, emphysema and chronic obstructive pulmonary disease.1,2) The drug is given orally and/or by injection or inhalation, thus producing relaxation of bronchial smooth muscle, inhibition of the release of endogenous spasmogens, inhibition of edema caused by endogenous mediators, increased mucociliary clearance and relaxation of the uterine muscle.3) In some respiratory diseases, it can often be taken in overdoses which can cause tremor, tachycardia, hypokalamia and sometimes fatal consequences. 4) For this reason, trace analysis of terbutaline is important in pharmaceutical research and clinical chemistry.Various techniques have been used for determination of terbutaline sulfate in its pharmaceutical formulations and biological fluids. These include capillary electrophoresis, [5][6][7][8][9][10][11] flow-injection chemiluminescence, 12,13) colorimetry, [14][15][16][17] spectrophotometry, [18][19][20] liquid chromatography, 21-24) gas chromatography, 25) high performance liquid chromatography [26][27][28][29][30][31][32][33] and cyclic voltammetry. 34) Most of the reported methods require time-consuming extraction steps prior to the analysis.Here a square-wave adsorptive anodic stripping voltammetric procedure was described for determination of terbutaline in bulk form, pharmaceutical formulation and human serum at a glassy carbon electrode. ExperimentalInstrumentation Computer-controlled Electrochemical Analyzers Models 263A and 394-PAR (Princeton Applied Research, Oak Ridge, TN., U.S.A.) were used for the voltammetric measurements. The electrode assembly (Model 303A-PAR) incorporated with a dark micro-electrolysis cell of three electrode system comprising of a glassy carbon disk electrode (G0197, 7 mm 2 surface area) as a working electrode, an Ag/AgCl/KCl s reference electrode and a platinum wire counter electrode, was used. Stirring of the solution in the micro-electrolysis cell was performed using a magnetic stirrer (305-PAR) with a star-shaped magnet to provide the convective transport during the preconcentration step. The whole measurements were automated and controlled through the programming capacity of the apparatus. The data were treated through a personal computer connected to the potentiostat and loaded with the 394 Analytical voltammetry software version 2.01-copyright © 1994 (PAR). A Mettler balance (Toledo-AB104, Greifensee, Switzerland) was used for weighing the solid materials. A pH-meter (Crison, Barcelona, Spain) was used for the pH measurements of the supporting electrolytes. A centrifuge (Eppendorf-5417 C, Hamburg, Germanny) was used for separation of the precipitated proteins from the human serum samples before assay of the drug. A micopipetter (Eppendorf-Multipette ® plus) was used for transfer of the reactant solutions throughout the present experimental work. The ...

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Stability indicating HPTLC method for determination of terbutaline Sulfate in bulk and from Submicronized dry Powder inhalers

“…1,2 A literature survey revealed that various analytical methods have been reported for the estimation of TBS; they include UV spectroscopy, 3,4 voltammetry, 5,6 capillary electrophoresis, [7][8][9] chemiluminescence, [10][11][12] high performance liquid chromatography (HPLC), 1,13,14 HPLC-fluorescence detection, 15 HPLC-electrochemical detection, 16,17 HPLCchemiluminescence detection, 18 RP-liquid chromatography, 19 LC-electrochemical detection, 20 column-switching LC, 21 LC-tandem mass spectrometry, 22,23 and high-resolution GC-mass spectrometry analysis. 24 For TBS pharmacokinetic studies, the most useful analytical method is GC-mass spectrometry.…”

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