High-performance liquid chromatographic analysis of baclofen in plasma and urine of man after precolumn extraction and derivatization with o-phthaldialdehyde

Wuis, E.W.; Dirks, Rita; Vree, T. B.; Kleyn, E. Van Der

doi:10.1016/0378-4347(85)80047-5

Cited by 49 publications

(12 citation statements)

References 21 publications

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“…Racemic baclofen in plasma and urine was measured by high pressure liquid chromatography (HPLC) with fluorimetric detection [6]. The minimum detectable concentration was 0.02mg.1-1.…”

Section: Analysis Of Plasma and Urinementioning

confidence: 99%

Pharmacokinetics of baclofen in spastic patients receiving multiple oral doses

Wuis

Dirks

Vree

et al. 1990

Pharmaceutisch Weekblad Scientific Edition

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The pharmacokinetics of racemic baclofen as determined from plasma and urine data in six spastic patients treated with individualized oral doses, 30-80 mg daily, are presented. Peak plasma concentrations were achieved 1.9 h (+/- 0.7) after a dose. The fluctuation in the plasma concentration was great, ranging from 188 to 439%. The total body clearance averaged 175 ml.min-1 (+/- 44), plasma protein binding 35% (+/- 6). Baclofen was for the greater part excreted unchanged by the kidney, 65% (+/- 16). Its apparent renal equalled the creatinine clearance. The contribution of the renal clearance to the total body clearance can explain the previously described toxicity when renal impairment is present. The results agree with earlier reports on single doses in healthy subjects.

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“…Racemic baclofen in plasma and urine was measured by high pressure liquid chromatography (HPLC) with fluorimetric detection [6]. The minimum detectable concentration was 0.02mg.1-1.…”

Section: Analysis Of Plasma and Urinementioning

confidence: 99%

Pharmacokinetics of baclofen in spastic patients receiving multiple oral doses

Wuis

Dirks

Vree

et al. 1990

Pharmaceutisch Weekblad Scientific Edition

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“…20±420 ng/mL). This range sufficiently covers the normal drug level encountered in plasma (0±300 ng/mL) following clinical administration of 20 mg of baclofen to a healthy adult [7,9]. Typical parameters for the calibration graphs in plasma were y = 3.2´10 5 x ± 6.0´10 3 , r = 0.9989 for S-(+)-baclofen and y = 3.1´10 5 x ± 4.1´10 3 , r = 0.9995 for R-(±)-baclofen, where y denotes the corrected peak area, x the concentration in mM and r the correlation coefficient.…”

Section: Calibration and Methods Validationmentioning

confidence: 99%

Chiral analysis of baclofen by α-cyclodextrin-modified capillary electrophoresis and laser-induced fluorescence detection

2001

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An enantioselective method for baclofen (4-amino-3-p-chlorophenylbutyric acid) based on capillary electrophoresis (CE) separation and laser-induced fluorescence (LIF) detection has been developed. Naphthalene-2,3-dicarboxaldehyde (NDA) was used for precolumn derivatization of the nonfluorescent drug. alpha-Cyclodextrin (alpha-CD) was included in the buffer as a chiral selector for the separation of NDA-labeled S-(+)- and R-(-)-baclofen. Optimal resolution and detection were obtained with an electrophoretic buffer of 50 mM sodium borate (pH 9.5) containing 7 mM alpha-CD and a He-Cd laser (lambda ex = 442 nm, lambda em = 500 nm). Combined with a simple cleanup procedure, this method can be applied to the analysis of baclofen enantiomers in human plasma. The relative standard deviation (RSD) values on peak areas of a plasma sample containing 1.0 microM racemic baclofen were 6.4 and 4.9% (n = 8) for the S-(+)- and R-(-)-enantiomer, respectively. The RSD value on migration times of both enantiomers was 0.5% (n = 8). Calibration graphs for S-(+)- and R-(-)-baclofen in plasma showed a good linearity (r > or = 0.999) in the concentration range of 0.1-2.0 microM. The limit of detection of baclofen in plasma was about 10 ng/mL.

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“…The detection limit of baclofen in plasma was estimated to be about 4.8 laM (SIN 3). However, the detection limit is not sufficient for the normal drug level encountered in plasma (0 300ng.mL 1) following clinical administration of 20 mg of baclofen to a healthy adult [10,12]. CE analysis preceded by an appropriate sample pretreatment procedure (e.g., solid phase extraction) is probably a solution for improving the detection limit.…”

Section: Applicationmentioning

confidence: 97%

“…Lengthy extraction and deanup procedures are required for the analysis of real samples. HPLC techniques with pro-column derivatization offer more variability, o-Phthaldialdehyde (OPA) [10,11] and 4-chloro-7-nitrobenzofurazan [12] have been used as derivatizing agents for fluorescence detection. In addition, LC-tandem MS with solid phase extraction was reported for the determination of baclofen in plasma [13].…”

Section: Introductionmentioning

confidence: 99%

Determination of baclofen by derivatization with anthracene-2,3-dicarboxaldehyde followed by capillary electrophoresis with laser-induced fluorescence detection

Chang

Yang

2003

Chromatographia

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KeyWordsCa pilla ry electrophoresis Laser-induced fluorescence detectTon DerTvatizatTon with anthracene-2,3-dicorboxaldehyde Baclofen Human plasma SummaryA new derTvatizatTon method has been developed for the sensitTve and selective determTnatTon of baclofen (4-amino-3-p-chlorophenylbutyrTc add). Anthracene-2,3-dicarboxaldehyde (ADA) was employed to form a stable conjugate of baclofen whose excitation wavelength is red-shifted from the orTginal reagent. The ADA-baclofen derTvative was separated by cepTIlary electrophoresis and detected wth a green HeNe laser-induced fluorescence detecton Linearity of more than iwo orders of magnTtudes was generally obtained. The concentration ITmTt of detection (CLOD) was in the 10 8 M range. A derivatization limit of detection of 0.3 u.M was achieved for baclofen. The precisions of the migration times and peak areas were 1.0% and 4.2%, respectively The applicability of the method for the determination of baclofen in human plasma was examined.

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High-performance liquid chromatographic analysis of baclofen in plasma and urine of man after precolumn extraction and derivatization with o-phthaldialdehyde

Cited by 49 publications

References 21 publications

Pharmacokinetics of baclofen in spastic patients receiving multiple oral doses

Pharmacokinetics of baclofen in spastic patients receiving multiple oral doses

Chiral analysis of baclofen by α-cyclodextrin-modified capillary electrophoresis and laser-induced fluorescence detection

Determination of baclofen by derivatization with anthracene-2,3-dicarboxaldehyde followed by capillary electrophoresis with laser-induced fluorescence detection

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