Determination of baclofen by derivatization with anthracene-2,3-dicarboxaldehyde followed by capillary electrophoresis with laser-induced fluorescence detection

Chang, Sarah Y.; Yang, Hsi-Hsien

doi:10.1007/bf02491772

Cited by 11 publications

(8 citation statements)

References 23 publications

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“…ADA has rarely been used, although it has a suitable excitation maximum at 480 nm, thus can be efficiently excited with the commonly used argon‐ion or LED lasers. So far, only a limited number of articles have focused on derivatization with ADA both for CE [31‐33] and for HPLC [34, 35], despite the reagent being commercially available (Sigma‐Aldrich). As a result, it does not deserve a separate chapter.…”

Section: Homocyclic O‐dicarboxaldehydesmentioning

confidence: 99%

Homocyclic o‐dicarboxaldehydes: Derivatization reagents for sensitive analysis of amino acids and related compounds by capillary and microchip electrophoresis

Celá

Glatz

2020

Electrophoresis

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Amino acids are essential compounds for living organisms, and their determination in biological fluids is crucial for the clinical analysis and diagnosis of many diseases. However, the detection of most amino acids is hindered by the lack of a strong chromophore/fluorophore or electrochemically active group in their chemical structures. The highly sensitive determination of amino acids often requires derivatization. Capillary electrophoresis is a separation technique with excellent characteristics for the analysis of amino acids in biological fluids. Moreover, it offers the possibility of precapillary, on‐capillary, or postcapillary derivatization. Each derivatization approach has specific demands in terms of the chemistry involved in the derivatization, which is discussed in this review. The family of homocyclic o‐dicarboxaldehyde compounds, namely o‐phthalaldehyde, naphthalene‐2,3‐dicarboxaldehyde, and anthracene‐2,3‐dicarboxaldehyde, are powerful derivatization reagents for the determination of amino acids and related compounds. In the presence of suitable nucleophiles they react with the primary amino group to form both fluorescent and electroactive derivatives. Moreover, the reaction rate enables all of the derivatization approaches mentioned above. This review focuses on articles that deal with using these reagents for the derivatization of amino acids and related compounds for ultraviolet‐visible spectrometry, fluorescence, or electrochemical detection. Applications in capillary and microchip electrophoresis are summarized and discussed.

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Section: Homocyclic O‐dicarboxaldehydesmentioning

confidence: 99%

Homocyclic o‐dicarboxaldehydes: Derivatization reagents for sensitive analysis of amino acids and related compounds by capillary and microchip electrophoresis

Celá

Glatz

2020

Electrophoresis

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“…To date, Bac has been determined in various biological materials using techniques based on gas (Giovanni & d'Aloja, ; Kochak & Hunc, ) or liquid (Ban, Park, & Kim, ; Fraser, MacNeil, & Isner, ; Tosunoglu & Ersoy, ) chromatography, liquid chromatography–tandem mass spectrometry (Di Rago et al, ; Dukova et al, ; Flardh & Jacobson, ; Sørensen & Hasselstrøm, ), HPLC with UV–vis and fluorescent detection (Cao & Li, ; Harrison, Tonkin, & Mclean, ), HPLC‐fluorescence detection after derivatization (Tosunoglu & Ersoy, ; Wuis, Dirks, Vree, & van der Kleyn, ) and HPLC‐electrochemical detection (Millerioux, Brault, Gualano, & Mignot, ). In addition, separation of naphthalene‐2,3‐dicarboxaldehyde‐derivatves of ( RS )‐Bac has been obtained (Chang & Yang, ; Chang, Zheng, & Chen, ; Chiang, Chang, & Whang, , ) using capillary electrophoresis (CE), and a chiral capillary column was employed to separate N ‐heptafluorobutyryl isobutyl ester derivatives of ( RS )‐Bac in human plasma and urine (Sioufi, Kaiser, Leroux, & Dubois, ). Enantiomers of Bac have been determined in pharmaceutical formulations using enantioselective membrane electrodes based upon maltodextrin and α ‐ and β ‐cyclodextrins (Rat'ko & Stefan‐van Staden, ; Stefan‐van Staden & Rat'ko, ).…”

Section: Introductionmentioning

confidence: 99%

Enantioresolution of (RS)‐baclofen by liquid chromatography: A review

Batra

Bhushan

2016

Biomedical Chromatography

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Baclofen is a commonly used racemic drug and has a simple chemical structure in terms of the presence of only one stereogenic center. Since the desirable pharmacological effect is in only one enantiomer, several possibilities exist for the other enantiomer for evaluation of the disposition of the racemic mixture of the drug. This calls for the development of enantioselective analytical methodology. This review summarizes and evaluates different methods of enantioseparation of (RS)-baclofen using both direct and indirect approaches, application of certain chiral reagents and chiral stationary phases (though very expensive). Methods of separation of diastereomers of (RS)-baclofen prepared with different chiral derivatizing reagents (under microwave irradiation at ease and in less time) on reversed-phase achiral columns or via a ligand exchange approach providing high-sensitivity detection by the relatively less expensive methods of TLC and HPLC are discussed. The methods may be helpful for determination of enantiomers in biological samples and in pharmaceutical formulations for control of enantiomeric purity and can be practiced both in analytical laboratories and industry for routine analysis and R&D activities.

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“…carboxaldehyde (ADA) [15], and naphthalene-2,3-dicarboxaldehyde (NDA) [16]. However, these probes show many shortcomings such as low fluorescence quantum yield, low stability, and so on.…”

Section: Introductionmentioning

confidence: 99%

Rapid and sensitive analysis of baclofen by high-performance liquid chromatography with UV-Vis and FD detection

Cao

2012

Acta Chromatographica

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Summary. Two rapid, sensitive and reproducible methods for the determination of baclofen(BAL) in urine and plasma based on high-performance liquid chromatography (HPLC) with UV-vis and fluorescent detection, respectively, were developed for the first time using a new synthesized fluorescent label, 6-oxy-(N-succinimidylacetate)-9-(2'-methoxycarbonyl) fluorescein (SAMF). The optimal derivatization yield was achieved in borate buffer (pH 8.0) for 15 min at room temperature (25 °C). With a mixture of methanol and water containing 5 mmol L −1 sodium citrate buffer (pH 5.0) as mobile phase, BAL was determined at λ = 455 nm with UV-vis detection and at λ ex /λ em = 488/520 nm with FD detection. The detection limits are 1.065 × 10 −3 mg mL −1 and 1.065 × 10 −2 mg mL −1 with HPLC-UV-vis and HPLC-FD, respectively. The proposed method has been successfully applied to the analysis of BAL in human urine and plasma samples. The established method is rapid (15 min of derivatization process and 10 min of chromatographic run), reproducible and sensitive.

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Determination of baclofen by derivatization with anthracene-2,3-dicarboxaldehyde followed by capillary electrophoresis with laser-induced fluorescence detection

Cited by 11 publications

References 23 publications

Homocyclic o‐dicarboxaldehydes: Derivatization reagents for sensitive analysis of amino acids and related compounds by capillary and microchip electrophoresis

Homocyclic o‐dicarboxaldehydes: Derivatization reagents for sensitive analysis of amino acids and related compounds by capillary and microchip electrophoresis

Enantioresolution of (RS)‐baclofen by liquid chromatography: A review

Rapid and sensitive analysis of baclofen by high-performance liquid chromatography with UV-Vis and FD detection

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