High-performance liquid chromatographic analysis of aminoglycoside antibiotics

Tawa, Riichi; Matsunaga, Hirokazu; Fujimoto, Takashi

doi:10.1016/s0021-9673(98)00342-2

Cited by 59 publications

(27 citation statements)

References 36 publications

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“…Various methods are available for the determination of aminoglycoside antibiotics, such as microbiological methods, enzyme immunoassays and chromatographic analyses. Microbiological assays are inexpensive and simple, but time consuming and nonspecific [3], enzyme immunoassays also lack versatility in routine multicomponent chemical analysis applications [3,4], and therefore LC is the most popular technique for the analysis of these compounds [3,[5][6][7]. In recent years, CE is increasingly being viewed as an alternative to LC for the determination of aminoglycoside antibiotics [3,8,9].…”

Section: Introductionmentioning

confidence: 99%

Trace analysis of aminoglycoside antibiotics in bovine milk by MEKC with LIF detection

Serrano

Silva

2006

Electrophoresis

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This work describes a straightforward and sensitive method for the multi-residue analysis of aminoglycoside antibiotics (kanamycin B, amikacin, neomycin B and paromomycin I) in bovine milk samples. The method involves the pre-capillary derivatization of antibiotics with sulfoindocyanine succinimidyl ester (Cy5) and their separation and determination by MEKC with LIF detection. The optimum procedure includes a derivatization step of the antibiotics at 25 degrees C for 30 min and direct injection for MEKC analysis, which is performed in about 20 min by using borate buffer (35 mM; pH 9.2) with 55 mM SDS as an anionic surfactant and 20% ACN as the organic modifier. Under these conditions, dynamic ranges of 10-500 microg/L and RSDs (within-day precision) from 3.8 to 5.3% were obtained. These results indicate that the proposed MEKC-LIF method is useful as a selective and sensitive tool for the determination of these antibiotics and surpasses other reported electrophoretic alternatives. Finally, the method was successfully applied to bovine milk samples after a simple solid-phase extraction clean-up and preconcentration procedure. The aminoglycosides were readily detected at 0.5-1.5 microg/kg levels with average recoveries ranging from 89.4 to 93.3%.

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Section: Introductionmentioning

confidence: 99%

Trace analysis of aminoglycoside antibiotics in bovine milk by MEKC with LIF detection

Serrano

Silva

2006

Electrophoresis

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“…They have been determined by bioassays, GC-MS and HPLC, in this case often following derivatization (to improve detection and/or separation), adding ionpair agents in the mobile phase or by ion-exchange techniques. More recently, HILIC was also proposed as worthwhile approach and there is an increasing number of applications; in literature brief reviews about the application of these methods for the separation of aminoglycoside antibiotics can be found (Tawa et al, 1998, Isoherranen and Sobak, 1999, Stead, 2000. Because of the nonvolatile and very polar nature of aminoglycosides the gas chromatographic analysis invariably involves derivatization of the amino and hydroxyl groups of these molecules.…”

Section: Chromatography Of Aminoglycosidesmentioning

confidence: 99%

HPLC-MS/MS of Highly Polar Compounds

Silvestro¹,

Tarcomnicu²,

Savu³

2012

Tandem Mass Spectrometry - Applications and Principles

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“…Reversed-phase HPLC (RP-HPLC) in particular, has proven to be very versatile in its ability to simultaneously analyze small molecular weight compounds of different polarities [60][61][62][63]. However, the cationic nature of weak basic drugs such as tricyclic antidepressants and bronchodilators produces broad asymmetrical peaks when analyzed by RP-HPLC or CEC with aqueous-organic solutions as a mobile phase, and C18 columns (containing high amount of residual or free silanol groups) as stationary phases [64,65].…”

Section: Determination Of Basic Drugs By Hplcmentioning

confidence: 99%

Characterization of pharmaceutical drugs by a modified nonaqueous capillary electrophoresis – mass spectrometry method

et al. 2003

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A simple method for the separation and characterization of a group of nine basic compounds, comprising seven tricyclic antidepressant and two bronchodilator drugs, by nonaqueous capillary electrophoresis (NACE) employing ultraviolet and mass spectrometry detection is described. After optimization of the electrophoresis separation conditions, including the compositions of the electrolyte and the organic solvent, a reliable separation of all nine basic analytes was achieved in 80 mM ammonium formate dissolved in a methanol-acetonitrite (80:20 v/v) mixture, having an apparent pH of 8.7. The volatile nonaqueous electrolyte system used with a normal electroosmotic flow polarity also provided an optimal separation condition for the characterization of the analytes by mass spectrometry. When results were compared with reversed-phase gradient and isocratic high-performance liquid chromatography (HPLC) methods, the NACE method provided greater efficiency, achieving baseline resolution for all nine basic compounds in less than 30 min. The NACE method is suitable for use as a routine procedure for the rapid separation and characterization of basic compounds and is a viable alternative to HPLC for the separation of a wide range of pharmaceutical drugs.

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High-performance liquid chromatographic analysis of aminoglycoside antibiotics

Cited by 59 publications

References 36 publications

Trace analysis of aminoglycoside antibiotics in bovine milk by MEKC with LIF detection

Trace analysis of aminoglycoside antibiotics in bovine milk by MEKC with LIF detection

HPLC-MS/MS of Highly Polar Compounds

Characterization of pharmaceutical drugs by a modified nonaqueous capillary electrophoresis – mass spectrometry method

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