High-performance glucose biosensor based on chitosan-glucose oxidase immobilized polypyrrole/Nafion/functionalized multi-walled carbon nanotubes bio-nanohybrid film

Shrestha, Bishnu Kumar; Ahmad, Rafiq; Mousa, Hamouda M.; Kim, In-Gi; Kim, Jeong In; Neupane, Madhav Prasad; Park, Chan Hee; Kim, Cheol Sang

doi:10.1016/j.jcis.2016.07.067

Cited by 121 publications

(45 citation statements)

References 51 publications

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“…In this context, the co-electrodepositon of pyrrole, graphene oxide, and auric ions (Au 3+ ) was performed by cyclic voltammetry in the potential ranging from −1.5 V to 0.8 V vs. a saturated calomel electrode (SCE) with a scan rate of 25 mV/s in HAuCl 4 ·4H 2 O aqueous solution for 10 cycles, as shown in Figure 2. The potential of 0.8 V vs. SCE is not only sufficient to allow the formation of the PPy polymer, but can also avoid the over-oxidation of the PPy [33]. It can be seen that the large reduction current should be due to graphene oxide and auric ions, since the reduction of water to hydrogen occurs at more negative potentials [16,34,35].…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Synthesis of Polypyrrole, Reduced Graphene Oxide, and Gold Nanoparticles Composite and Its Application to Hydrogen Peroxide Biosensor

Zhao

Hou

et al. 2016

Nanomaterials

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Here we report a facile eco-friendly one-step electrochemical approach for the fabrication of a polypyrrole (PPy), reduced graphene oxide (RGO), and gold nanoparticles (nanoAu) biocomposite on a glassy carbon electrode (GCE). The electrochemical behaviors of PPy–RGO–nanoAu and its application to electrochemical detection of hydrogen peroxide were investigated by cyclic voltammetry. Graphene oxide and pyrrole monomer were first mixed and casted on the surface of a cleaned GCE. After an electrochemical processing consisting of the electrooxidation of pyrrole monomer and simultaneous electroreduction of graphene oxide and auric ions (Au3+) in aqueous solution, a PPy–RGO–nanoAu biocomposite was synthesized on GCE. Each component of PPy–RGO–nanoAu is electroactive without non-electroactive substance. The obtained PPy–RGO–nanoAu/GCE exhibited high electrocatalytic activity toward hydrogen peroxide, which allows the detection of hydrogen peroxide at a negative potential of about −0.62 V vs. SCE. The amperometric responses of the biosensor displayed a sensitivity of 40 µA/mM, a linear range of 32 µM–2 mM, and a detection limit of 2.7 µM (signal-to-noise ratio = 3) with good stability and acceptable reproducibility and selectivity. The results clearly demonstrate the potential of the as-prepared PPy–RGO–nanoAu biocomposite for use as a highly electroactive matrix for an amperometric biosensor.

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Section: Resultsmentioning

confidence: 99%

Electrochemical Synthesis of Polypyrrole, Reduced Graphene Oxide, and Gold Nanoparticles Composite and Its Application to Hydrogen Peroxide Biosensor

Zhao

Hou

et al. 2016

Nanomaterials

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“…In the past decade, conducting polymers have wide applications in lightweight electrodes, sensors, electromagnetic shielding, and supercapacitors because of their electrochemical property, low cost, environmental stability and simple preparation . Environmental remediation with conducting polymer has also gained more attention due to its reversibility and high efficiency of Cr(VI) removal.…”

Section: Introductionmentioning

confidence: 99%

Facile Assembly of Polyaniline/Graphene Oxide Composite Hydrogels as Adsorbent for Cr(VI) Removal

et al. 2019

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A facile method for the assembly of polyaniline/graphene oxide (PANi/GO) composite hydrogel has been successfully demonstrated by in‐situ polymerization of aniline in the presence of phytic acid with the aid of GO. During the polymerization of aniline, the 2D GO nanosheets can regulate the aggregation state of polymer and prompt the formation of the hydrogel. The chemical structure, morphology and mechanical property are investigated by Fourier transform infrared spectroscopy, X‐ray diffraction, scanning electron microscopy and compressive stress–strain measurements, et al. Pani/GO composite hydrogel with high surface area and 3D porous nanostructures exhibits higher adsorption capability for Cr(VI) in compared with PANi hydrogel. Pseudo‐second‐order and Langmuir isotherm models are effectively employed to describe the adsorption behaviors of PANi/GO composite hydrogel. Furthermore, the regeneration experiments indicate that the as‐prepared composite hydrogel can remain 73.5% of the original removal capability after five adsorption–desorption cycles. It is expected that the PANi/GO composite hydrogel can be an effective candidate for the removal of Cr(VI) due to the synergistic effects between PANi and GO within the hydrogel network. POLYM. COMPOS., 40:E1777–E1785, 2019. © 2019 Society of Plastics Engineers

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“…These third generation biosensors are composed of electrodes (e.g., glassy carbon electrode, porous gold electrode, etc.) and are functionalized with composite materials such as sensing elements (e.g., carbon nanotubes [CNTs]) and immobilized GOx (du Toit & Di Lorenzo, ; M. Zhang et al, ; Sağlam, Kızılkaya, Uysal, & Dilgin, ; Shrestha et al, ; Suganthi, Arockiadoss, & Uma, ; Tian, Alex, Siegel, & Tiwari, ; X. P. Zhang, Liu, Li, & You, ; Xu, Sheng, Shen, & Zheng, ; Y. Yu et al, ). The presence of endogenous electroactive metabolites and impurities, the long distance between redox cofactor and electrode surface, and the orientation of GOx on the electrode, though, are difficult challenges for the development of direct electron transfer approaches (Bartlett & Al‐Lolage, ; Luong, Glennon, Gedanken, & Vashist, ).…”

Section: Introductionmentioning

confidence: 99%

How to engineer glucose oxidase for mediated electron transfer

Gutierrez

Wallraf

Balaceanu

et al. 2018

Biotech & Bioengineering

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Glucose oxidase (GOx) is of high industrial interest for glucose sensing because of its high β-d-glucose specificity. The efficient and specific electrochemical communication between the redox center and electrodes is crucial to ensure accurate glucose determination. The efficiency of the electron transfer rates (ETR) with GOx, together with quinone diamine based mediators, is low and differs even among mediator derivatives. To design optimized enzyme-mediator couples and to describe a mediator binding model, a joint experimental and computational study was performed based on an oxygen-independent GOx variant V7 and two quinone diimine based electron mediators (QDM-1 and QDM-2), which differ in polarity and size, and ferrocenemethanol (FM). A site saturation library at position 414 was screened with all three mediators and yielded four beneficial substitutions Tyr, Met, Leu, and Val. The variants showed increased mediator activity for the more polar QDM-2 with a simultaneously decreased activity for the less polar and smaller QDM-1 and for FM. The variant GOx V7-I414Y exhibited the biggest change for the quinone diimine derivatives compared with V7 (QDM-1: 55.9 U/mg V7, 33.2 U/mg V7-I414Y; QDM-2: 2.7 U/mg V7, 12.9 U/mg V7-I414Y). Theoretical ETR calculated based on the Marcus theory were in good agreement with the experimental results. Molecular docking studies revealed a preferable binding of the two QD mediators directly in the active site, 3.5 Å away from the N5 atom of the flavin adenine dinucleotide (FAD) and in direct vicinity to position 414. In summary, position 414 in the active site was identified to modulate the electron shuttling from the FAD of the GOx to small water-soluble mediators dependent on the polarity and size of residue 414 and on the polarity and size of the mediator. The presented mediator binding model offers a promising possibility for the design of optimized enzyme-mediator couples.

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High-performance glucose biosensor based on chitosan-glucose oxidase immobilized polypyrrole/Nafion/functionalized multi-walled carbon nanotubes bio-nanohybrid film

Cited by 121 publications

References 51 publications

Electrochemical Synthesis of Polypyrrole, Reduced Graphene Oxide, and Gold Nanoparticles Composite and Its Application to Hydrogen Peroxide Biosensor

Electrochemical Synthesis of Polypyrrole, Reduced Graphene Oxide, and Gold Nanoparticles Composite and Its Application to Hydrogen Peroxide Biosensor

Facile Assembly of Polyaniline/Graphene Oxide Composite Hydrogels as Adsorbent for Cr(VI) Removal

How to engineer glucose oxidase for mediated electron transfer

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