High‐performance detection of an abdominal aortic aneurysm biomarker by immunosensing

Guo, Shikui; Li, Yuejin; Li, Rougang; Zhang, Peng; Wang, Yongzhi; Gopinath, Subash C. B.; Gong, Ke; Wan, Ping

doi:10.1002/bab.1877

Cited by 7 publications

(5 citation statements)

References 28 publications

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“…The tetravalent streptavidin-biotin method used in this research yielded a good output and can be utilized for other biomarker analyses. Interdigitated electrode 0-2 V (current-volt) Immunosensing At low picomolar Dose-dependent analysis [33] Ultrasonic transducer 40 kHz Oscillator 2 to 400 cm Distance traveled [34] Telemetric pressure 95-130 mmHg Intra-aortic pressure 10 cm Transmission range [35] Telemetric pressure 133 kHz Operating frequency 120 mm Transmission range [36] Wireless pressure 10-20 MHz Operating frequency 40.27 kHz/mmHg Resonant frequency [37] Interdigitated electrode microwave 24 GHz Operating frequency 200 MHz and 4 GHz Frequency [38] Interdigitated electrode 0-2 V (current-volt) Duplex formation At low femtomolar Dose-dependent analysis Current work…”

Section: Discussionmentioning

confidence: 99%

Detection of microRNA-335-5p on an Interdigitated Electrode Surface for Determination of the Severity of Abdominal Aortic Aneurysms

Liu

Wang

et al. 2020

Nanoscale Res Lett

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Abdominal aortic aneurysm (AAA) refers to the enlargement of the lower artery of the abdominal aorta, and identification of an early detection tool is urgently needed for diagnosis. In the current study, an interdigitated electrode (IDE) sensing surface was used to identify miRNA-335-5p, which reflects the formation of AAAs. The uniformity of the silica material was observed by 3D profilometry, and the chemically modified highly conductive surface improved the detection via the I-V mode. The targeted miRNA-335-5p was detected in a dose-dependent manner and based on linear regression and 3σ analyses, the sensitivity was determined to be 1 fM with a biotinylated probe. The high specificity was shown by discriminating the target sequence from noncomplementary and single-and triple-mismatched sequences. These outputs demonstrated the high-performance detection of miRNA-335-5p with good reproducibility for determination of the severity of AAA.

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Section: Discussionmentioning

confidence: 99%

Detection of microRNA-335-5p on an Interdigitated Electrode Surface for Determination of the Severity of Abdominal Aortic Aneurysms

Liu

Wang

et al. 2020

Nanoscale Res Lett

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“…Abdominal aortic aneurysm (AAA) is a fatal arterial disease, defined by an abnormal bulge in the artery wall. AAA is generally developed by the weakened artery due to injury, infection, or congenital defect in the connective tissue of the artery [ 1 , 2 ]. Various other reasons also contribute to AAA, which include genetic susceptibility, atherosclerosis, smoking, and hypertension [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…AAA is generally developed in the abdominal aorta between the renal and iliac arteries [ 5 ], and a diameter of >3 cm causes various health issues [ 6 ]. In particular, if the aneurysm size expands above 5.5 cm, it ruptures and causes bleeding which leads to death [ 2 ]. AAA is a dynamic and complex pathophysiological process, and the molecular mechanism of AAA is still unclear.…”

Section: Introductionmentioning

confidence: 99%

“…The rupturing of AAA is asymptomatic, and the present imaging methods, such as ultrasound computerized tomography and magnetic resonance imaging, are helpful in identifying AAA, but these methods are inconvenient and expensive. So, it is mandatory to develop a sensing strategy to diagnose AAA by blood-based biomarker analysis [ 2 , 7 – 9 ]. Various blood-based biomarkers help to identify and diagnose the disease at its earlier stages, which helps to provide the proper treatment at the right time [ 10 – 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Current-Volt Biosensing “Cystatin C” on Carbon Nanowired Interdigitated Electrode Surface: A Clinical Marker Analysis for Bulged Aorta

Chen

Kang

Sun

et al. 2022

Journal of Analytical Methods in Chemistry

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A carbon nanowire-modified surface with interdigitated electrode (IDE) sensing system was introduced to identify abdominal aortic aneurysm biomarker “papain,” also known as cysteine protease, used as the capture probe to identify Cystatin C. Papain was immobilized through the covalent integration of amine group on papain and the carboxyl group with carbon nanowire. This papain-modified electrode surface was utilized to detect the different concentrations of Cystatin C (100 pg/mL to 3.2 ng/mL). The interaction between papain and Cystatin C was monitored using a picoammeter, and the response curves were compared. With increasing Cystatin C concentrations, the total current levels were gradually increased with a linear range from 200 pg/mL to 3.2 ng/mL, and the current differences were plotted and the detection limit of Cystatin C was calculated as 200 pg/mL. The averaging of three independent experiments (n = 3) was made with 3δ estimation, and the determination coefficient was y = 1.8477 × 0.7303 and R2 = 0.9878. Furthermore, control experiments with creatinine and gliadin failed to bind the immobilized papain, indicating the specific detection of Cystatin C.

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“…Improving the biosensor performance is essential to diagnose and monitor the diseases during and after the treatment [8][9][10][11]. Antibody-based immunoassays are commonly used for various diagnostic assays with sensors, which include enzymelinked immunoassay, RAMAN spectroscopy, surface plasmon resonance, wave-guide mode, and electrochemical system [12][13][14][15][16][17][18]. Moreover, recent years have witnessed improved immunoassay procedure that was developed by the signal enhancement using nanomaterials, antibody immobilization procedures, and novel lab-on-chip technologies.…”

Section: Introductionmentioning

confidence: 99%

Thyroglobulin determination on silane–antibody functionalized interdigitated dielectrode surface to diagnose thyroid tumor

Wang

Sun

Gopinath

et al. 2021

Biotech and App Biochem

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Thyroid cancer appears in endocrine glands and specific to thyroid glands has been reported widely. This work was targeted to identify and quantify thyroglobulin by using antithyroglobulin antibody complexed silane surface on interdigitated electrode (IDE) sensing surface.(3-Aminopropyl)triethoxysilane linker was used to make silane-coupling with antibody and attached on the hydroxylated IDE. This electroanalytical IDE revealed the dose-dependent responses with thyroglobulin concentrations. By getting increments with the thyroglobulin concentrations, the current responses were enhanced concomitantly and the thyroglobulin detection limit was noted as 1 pM on the linear curve [y = 0.1311x + 0.5386; R 2 = 0.9707] with the sensitivity at lower picomolar range. Moreover, the control experiments with thyroid peroxidase and nonimmune antibody cannot yield any response of current, confirming the specific detection of thyroglobulin. This research set-up is useful to determine and quantify the thyroglobulin and diagnose thyroid cancer.

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High‐performance detection of an abdominal aortic aneurysm biomarker by immunosensing

Cited by 7 publications

References 28 publications

Detection of microRNA-335-5p on an Interdigitated Electrode Surface for Determination of the Severity of Abdominal Aortic Aneurysms

Detection of microRNA-335-5p on an Interdigitated Electrode Surface for Determination of the Severity of Abdominal Aortic Aneurysms

Current-Volt Biosensing “Cystatin C” on Carbon Nanowired Interdigitated Electrode Surface: A Clinical Marker Analysis for Bulged Aorta

Thyroglobulin determination on silane–antibody functionalized interdigitated dielectrode surface to diagnose thyroid tumor

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