Detection of microRNA-335-5p on an Interdigitated Electrode Surface for Determination of the Severity of Abdominal Aortic Aneurysms

Liu, Bei; Liu, Leiting; Wang, Jian; Chen, Yuan; Li, Zhijiang; Gopinath, Subash C.B.; Lakshmipriya, Thangavel; Huo, Zongwei

doi:10.1186/s11671-020-03331-y

Cited by 21 publications

(15 citation statements)

References 38 publications

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“…Recently, Changwu et al reported miR‑26b‑5p regulated the transforming growth factor β (TGFβ)/Smad4 signaling pathway to modulate hypoxia‑induced phenotypic switching of VSMCs. On the other hand, harnessing the results of a prior study [ 53 ], Bei et al [ 54 ] designed a novel device that would determination of the severity of AAA by detecting miRNA-335-5p. However, little is known about the specific regulation network of these two miRNAs in the aneurysm.…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics analysis of common key genes and pathways of intracranial, abdominal, and thoracic aneurysms

Liu

et al. 2021

BMC Cardiovasc Disord

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Background Aneurysm is a severe and fatal disease. This study aims to comprehensively identify the highly conservative co-expression modules and hub genes in the abdominal aortic aneurysm (AAA), thoracic aortic aneurysm (TAA) and intracranial aneurysm (ICA) and facilitate the discovery of pathogenesis for aneurysm. Methods GSE57691, GSE122897, and GSE5180 microarray datasets were downloaded from the Gene Expression Omnibus database. We selected highly conservative modules using weighted gene co‑expression network analysis before performing the Gene Ontology, Kyoto Encyclopedia of Genes and Genomes pathway and Reactome enrichment analysis. The protein–protein interaction (PPI) network and the miRNA-hub genes network were constructed. Furtherly, we validated the preservation of hub genes in three other datasets. Results Two modules with 193 genes and 159 genes were identified as well preserved in AAA, TAA, and ICA. The enrichment analysis identified that these genes were involved in several biological processes such as positive regulation of cytosolic calcium ion concentration, hemostasis, and regulation of secretion by cells. Ten highly connected PPI networks were constructed, and 55 hub genes were identified. In the miRNA-hub genes network, CCR7 was the most connected gene, followed by TNF and CXCR4. The most connected miRNAs were hsa-mir-26b-5p and hsa-mir-335-5p. The hub gene module was proved to be preserved in all three datasets. Conclusions Our study highlighted and validated two highly conservative co-expression modules and miRNA-hub genes network in three kinds of aneurysms, which may promote understanding of the aneurysm and provide potential therapeutic targets and biomarkers of aneurysm.

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Section: Discussionmentioning

confidence: 99%

Bioinformatics analysis of common key genes and pathways of intracranial, abdominal, and thoracic aneurysms

Liu

et al. 2021

BMC Cardiovasc Disord

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“…miRNA can bind specifically with its target mRNA in order to inhibit or decrease the expression. Recently, miRNA is more popularized and developing rapidly; in particular, the circulating miRNAs were found as the reliable modern biomarker for disease identification [ 21 ]. miRNA-137, containing 23 nucleotides, is found as the noninvasive reliable biomarker for AD [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Graphene Oxide-Gold Star Construct on Triangular Electrodes for Alzheimer’s Disease Identification

Chang

Zhao

Liu

et al. 2021

Journal of Analytical Methods in Chemistry

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Nanotechnology is playing a major role in the field of medical diagnosis, in particular with the biosensor and bioimaging. It improves the performance of the desired system dramatically by displaying higher selectivity and sensitivity. Carbon nanomaterial, gold nanostructure, magnetite nanoparticle, and silica substrate are the most popular nanomaterials greatly contributed to make the affordable and effective biosensor at low-cost. This research work is introducing a new sensing strategy with graphene oxide-constructed triangular electrodes to diagnose Alzheimer’s disease (AD). MicroRNA-137 (miRNA-137) was found as a suitable biomarker for AD, and the sensing method was established here to detect miRNA-137 on the complementary sequence. To enhance the immobilization of capture miRNA-137, gold nanostar (GNS) was conjugated with capture miRNA and immobilized on the GO-modified surface through an amine linker. This immobilization process enhanced the hybridization of the target and reaches the detection limit at 10 fM with the sensitivity of 1 fM on the linear curve with a regression coefficient of 0.9038. Further control sequences of miRNA-21 and single and triple base mismatched miRNA-137 did not show a significant response in current changes, indicating the specific miRNA-137 detection for diagnosing AD.

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“…It may be difficult to achieve reproducibility and biocompatibility of immobilized biomolecules by the above immobilization methods. In particular, the attachment of smaller capture molecules such as RNA, DNA, aptamers, and peptides to sensing surfaces is complicated [ 7 , 8 ]. Various researchers have used different techniques for efficient immobilization.…”

Section: Introductionmentioning

confidence: 99%

Longitudinal Zeolite-Iron Oxide Nanocomposite Deposited Capacitance Biosensor for Interleukin-3 in Sepsis Detection

Chen¹,

Gopinath²,

Anbu³

2021

Nanoscale Res Lett

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Sepsis is an extreme condition involving a physical response to severe microbial infection and causes fatal and life-threatening issues. Sepsis generates during the chemicals release with the immune system into the bloodstream for fighting against an infection, which causes the inflammation and leads to the medical emergency. A complexed longitudinal zeolite and iron oxide nanocomposite was extracted from coal mine fly ash and utilized to improve the surface characteristics of the capacitance biosensor to identify sepsis attacks. Anti-interleukin-3 (anti-IL-3) antibody was attached to the zeolite- and iron oxide-complexed capacitance electrode surface through an amine linker to interact with the sepsis biomarker IL-3. The morphological and chemical components of the nanocomplex were investigated by FESEM, FETEM, and EDX analyses. At approximately 30 nm, the longitudinal zeolite and iron oxide nanocomposite aided in attaining the limit of IL-3 detection of 3 pg/mL on the linear curve, with a regression coefficient (R2) of 0.9673 [y = 1.638x − 1.1847]. A lower detection limit was achieved in the dose-dependent range (3–100 pg/mL) due to the higher amount of antibody immobilization on the sensing surface due to the nanomaterials and the improved surface current. Furthermore, control experiments with relevant biomolecules did not show capacitance changes, and spiked IL-3 in human serum increased capacitance, indicating the specific and selective detection of IL-3. This study identifies and quantifies IL-3 via potentially useful methods and helps in diagnosing sepsis attack.

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Detection of microRNA-335-5p on an Interdigitated Electrode Surface for Determination of the Severity of Abdominal Aortic Aneurysms

Cited by 21 publications

References 38 publications

Bioinformatics analysis of common key genes and pathways of intracranial, abdominal, and thoracic aneurysms

Bioinformatics analysis of common key genes and pathways of intracranial, abdominal, and thoracic aneurysms

Graphene Oxide-Gold Star Construct on Triangular Electrodes for Alzheimer’s Disease Identification

Longitudinal Zeolite-Iron Oxide Nanocomposite Deposited Capacitance Biosensor for Interleukin-3 in Sepsis Detection

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