High outcrossing and random pollen dispersal in a planted stand of Acacia saligna subsp. saligna revealed by paternity analysis using microsatellites

Millar, Melissa A.; Byrne, Margaret; Nuberg, Ian; Sedgley, Margaret

doi:10.1007/s11295-007-0115-z

Cited by 37 publications

(45 citation statements)

References 30 publications

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“…Generalist insects including native and introduced bees are known to routinely fly distances of several kilometres, especially among fragmented habitat (Dick, 2001;Jha and Dick, 2010;Hagler et al, 2011) and may directly facilitate pollen dispersal when traversing intervening unsuitable habitat among populations. Pollen dispersal over a distance of 1.6 km has also been recorded previously for A. saligna, a common widespread species of the more mesic areas of south west WA, using direct paternity analysis methods (Millar et al, 2008.…”

Section: Discussionsupporting

confidence: 63%

Genetic connectivity and diversity in inselberg populations of Acacia woodmaniorum, a rare endemic of the Yilgarn Craton banded iron formations

2013

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Historically rare plant species with disjunct population distributions and small population sizes might be expected to show significant genetic structure and low levels of genetic diversity because of the effects of inbreeding and genetic drift. Across the globe, terrestrial inselbergs are habitat for rich, often rare and endemic flora and are valuable systems for investigating evolutionary processes that shape patterns of genetic structure and levels of genetic diversity at the landscape scale. We assessed genetic structure and levels of genetic diversity across the range of the historically rare inselberg endemic Acacia woodmaniorum. Phylogeographic and genetic structure indicates that connectivity is not sufficient to produce a panmictic population across the limited geographic range of the species. However, historical levels of gene flow are sufficient to maintain a high degree of adaptive connectivity across the landscape. Genetic diversity indicates gene flow is sufficient to largely counteract any negative genetic effects of inbreeding and random genetic drift in even the most disjunct or smallest populations. Phylogeographic and genetic structure, a signal of isolation by distance and a lack of evidence of recent genetic bottlenecks suggest long-term stability of contemporary population distributions and population sizes. There is some evidence that genetic connectivity among disjunct outcrops may be facilitated by the occasional long distance dispersal of Acacia polyads carried by insect pollinators moved by prevailing winds. Keywords: gene flow; inselberg; isolation; landscape; phylogeographic structure INTRODUCTION For plant species, demographic and genetic connectivity is affected by seed and pollen dispersal vectors, by a number of life history traits including life form, longevity and the mating system, and by the size and spatial arrangement of populations. Rare plant species and those with specific habitat requirements often have geographically disjunct and small population sizes. For these species, the surrounding landscape typically comprises a matrix of unsuitable habitat that acts as an effective physical barrier to dispersal and the size and spatial arrangement of populations assumes an important role in the structuring and maintenance of genetic diversity. Limitations to connectivity result in a degree of demographic isolation and genetic isolation among populations (Slatkin, 1987), and produce the phylogeographic and genetic structuring observed in many habitat specific, rare, endemics (Yates et al., 2007;Byrne and Hopper, 2008;Butcher et al., 2009). Limited connectivity among disjunct populations can result in reduced levels of genetic diversity as a result of increased levels of inbreeding and via the negative impacts of genetic drift (Slatkin, 1987;Ellstrand, 1992), both of which are further enhanced in small populations. Structuring and maintenance of genetic diversity has a key role in the ultimate persistence of species as they evolve and adapt to changing conditions. As a result of ...

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Section: Discussionsupporting

confidence: 63%

Genetic connectivity and diversity in inselberg populations of Acacia woodmaniorum, a rare endemic of the Yilgarn Craton banded iron formations

2013

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“…Kebun benih merupakan tempat untuk produksi berlimpah benih pohon-pohon yang unggul sesuai dengan karakter yang diinginkan (Millar et al, 2008). Penelitian mengenai kebun benih antara lain mengenai keuntungan genetik (genetic gains) pada kebun benih generasi pertama (Weng et al, 2008), analisis tetua (Hansen and Kjaer, 2006), kontaminasi serbuk sari dan sistem perkawinan (Slavov et al, 2005;Kaya et al, 2006) telah dilakukan.…”

Section: Pendahuluanunclassified

“…Penanda ISSR (Chezhian et al, 2010), kombinasi antara RAPD dan ISSR (Josiah et al, 2008), RAPD dan mikrosatelit (Shiran et al, 2007), serta mikrosatelit (Butcher et al, 2004;Zelener et al, 2005;Jones et al, 2006;Nurtjajaningsih et al, 2007;Millar et al, 2008) telah digunakan untuk mendeteksi keragaman genetik di kebun benih. Penanda mikrosatelit umumnya digunakan karena penanda ini sangat polimorfik, bersifat codominan, netral dan dapat diamplifikasi dengan sejumlah kecil DNA (Millar et al, 2008).…”

Section: Pendahuluanunclassified

KERAGAMAN GENETIK TETUA DAN ANAKAN DARI KEBUN BENIH SEMAI Acacia mangium GRUP D (AM004) DI SUMATERA SELATAN, INDONESIA

Yuskianti¹

2014

JPKW

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ABSTRAKInformasi keragaman genetik dari satu generasi ke generasi selanjutnya merupakan faktor penting untuk pengelolaan dan konservasi genetik kebun benih. Tujuan dari penelitian ini adalah untuk mengevaluasi tingkat keragaman genetik tetua sebagai generasi pertama (F1) dan anakannya (F2) dari kebun benih Akasia mangium yang ada di kebun benih semai Grup D (AM004) di Sumatera Selatan. Analisis dilakukan terhadap 251 tetua dan ± 200 biji dari 10 pohon induk. Analisis menggunakan 12 penanda mikrosatelit menunjukkan bahwa rata-rata jumlah alel yang terdeteksi (A) dari semua tetua dan anakannya adalah 8,23 dan 7.08. Secara umum, tingkat keragaman genetik tetua dan anakan tidak berbeda (He=0.609 untuk tetua dan He=0.606 untuk anakan). Penelitian ini mendeteksi keberadaan alel baru pada anakan. Kondisi ini menunjukkan kemungkinan adanya kontaminasi serbuk sari dari luar kebun benih.Kata kunci: Keragaman genetik, kebun benih semai Sumatera Selatan, tetua, anakan, penanda mikrosatelit ABSTRACT Information on genetic diversity from one generation to its next generation is an important factor for management and conservation in a seed orchard. The purpose of this study was to evaluate the level of genetic diversity of parental as the first generation (F1) and offspring (F2) of Acacia mangium in the

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“…As part of a larger process of assessing the risk posed by large-scale utilisation of A. saligna as an agroforestry crop to natural remnant populations of A. saligna, we developed and screened microsatellite markers for use in A. saligna [15].…”

Section: Introductionmentioning

confidence: 99%

Pollen contamination in Acacia saligna: assessing the risks for sustainable agroforestry

Millar¹,

Byrne²

2007

WIT Transactions on Ecology and the Environment, Vol 106

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Species developed for sustainable agroforestry may pose risks to remnant populations of closely related species via genetic contamination. Genetic contamination and the production of hybrid progeny may threaten the health and long-term viability of remnant populations. Acacia saligna is a native Western Australian species complex selected for further development for agroforestry in the agricultural areas of southern Australia.A. saligna shows great morphological, ecological, biological and genetic variation, and will be reclassified into a number of subspecies. This research aimed to develop genetic markers and use them to assess the levels and distances of gene flow via pollen dispersal between two of the proposed subspecies of Acacia saligna. Pollen dispersal from the abundantly flowering subsp. saligna into the poorer flowering subsp. lindleyi was high (32%). At the same site, pollen dispersal from subsp. lindleyi into subsp. saligna was much lower (14%). Most genetic contamination from subsp. saligna into subsp. lindleyi occurred at short distances (<300 m) although inter-subspecific pollen dispersal was detected over large distances of around 1600 m. The comparative levels of inter-subspecific pollen dispersal are influenced by the relative floral fecundity of the different variants. The results indicate that small remnant populations of subsp. lindleyi will be exposed to high levels of hybridisation when subsp. saligna is planted nearby for agroforestry. The effects of inter-subspecific hybridisation in A. saligna are unknown and until further investigated it is recommended as a management guideline that isolation distances of 1600 m or more be imposed between agroforestry plantings and native populations.

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High outcrossing and random pollen dispersal in a planted stand of Acacia saligna subsp. saligna revealed by paternity analysis using microsatellites

Cited by 37 publications

References 30 publications

Genetic connectivity and diversity in inselberg populations of Acacia woodmaniorum, a rare endemic of the Yilgarn Craton banded iron formations

Genetic connectivity and diversity in inselberg populations of Acacia woodmaniorum, a rare endemic of the Yilgarn Craton banded iron formations

KERAGAMAN GENETIK TETUA DAN ANAKAN DARI KEBUN BENIH SEMAI Acacia mangium GRUP D (AM004) DI SUMATERA SELATAN, INDONESIA

Pollen contamination in Acacia saligna: assessing the risks for sustainable agroforestry

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