High molecular weight hyaluronic acid reduces the growth and biofilm formation of the oral pathogen Porphyromonas gingivalis

Alharbi, Meshal S.; Alshehri, Fahad A.; Alobaidi, Ahmed S.; AlRowis, Raed; Al‐Shibani, Nouf; Niazy, Abdurahman A.

doi:10.1016/j.sdentj.2023.01.008

Cited by 3 publications

(6 citation statements)

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“…The antibacterial effect of HA against P. gingivalis has been demonstrated by multiple laboratory studies. HA was shown to significantly inhibit the growth and biofilm formation of P. gingivalis in vitro [ 28 , 29 , 30 ]. It was also able to significantly reduce the expression of multiple virulent genes in P. gingivalis, including fimA, mfa1, hagA, rgpA , and hagA [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

“…While the antimicrobial effect of HA against P. gingivalis has been demonstrated in laboratory studies [ 28 , 29 , 30 , 31 ], its effect on other oral species remains unclear. This is especially important regarding the normal flora and other beneficial species, as the introduction of antibiotics or antimicrobials into the biofilm environment could possibly lead to a loss of the functional balance within the biofilm and the creation of a dysbiotic state [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

“…Previously, a systematic review evaluating the adjunctive use of HA during surgical and non-surgical periodontal treatments suggested that HA may have additional clinical benefits, including probing depth reduction, clinical attachment gain, and reduced bleeding on probing [42]. While the antimicrobial effect of HA against P. gingivalis has been demonstrated in laboratory studies [28][29][30][31], its effect on other oral species remains unclear. This is especially important regarding the normal flora and other beneficial species, as the introduction of antibiotics or antimicrobials into the biofilm environment could possibly lead to a loss of the functional balance within the biofilm and the creation of a dysbiotic state [43].…”

Section: Discussionmentioning

confidence: 99%

“…Binshabaib and colleagues demonstrated that a gel containing 0.8% HA reduced colony-forming units of P. gingivalis grown on glass slides more significantly than 0.2% chlorhexidine (CHX) after 48 and 72 h [ 29 ]. Another in vitro study showed that high-molecular-weight HA significantly reduced the growth and biofilm formation of P. gingivalis [ 30 ]. HA was also shown to significantly reduce the expression of multiple genes related to adhesion and virulence in P. gingivalis, including fimA, mfa1, hagA, rgpA , and kgp [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Adjunctive Use of Hyaluronic Acid on Prevalence of Porphyromonas gingivalis in Subgingival Biofilm in Patients with Chronic Periodontitis: A Systematic Review

Alshehri

Alharbi

2023

Pharmaceutics

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Porphyromonas gingivalis (P. gingivalis) is a Gram-negative anaerobic bacterium that plays an important role in the development and progression of periodontitis. Hyaluronic acid (HA) is a naturally occurring glycosaminoglycan that has previously demonstrated antibacterial potential in vitro against multiple bacterial species, including P. gingivalis. The purpose of this systematic review is to evaluate the effectiveness of HA as an adjunctive topical antibacterial agent to non-surgical mechanical therapy of periodontitis in reducing the prevalence of P. gingivalis in subgingival biofilms. Five clinical studies were identified that satisfied the eligibility criteria. Only three trials were suitable for the meta-analysis as they provided data at three and six months. Data on the prevalence of P. gingivalis in each study were collected. The odds ratio (OR) for measuring the effect size with a 95% confidence interval (CI) was applied to the available data. The results did not favor the use of HA during non-surgical mechanical therapy to reduce the prevalence of P. gingivalis in subgingival biofilm (odd ratio = 0.95 and 1.11 at three and six months, consecutively). Within their limitations, the current data do not indicate an advantage for using HA during mechanical periodontal therapy to reduce the prevalence of P. gingivalis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Effect of Adjunctive Use of Hyaluronic Acid on Prevalence of Porphyromonas gingivalis in Subgingival Biofilm in Patients with Chronic Periodontitis: A Systematic Review

Alshehri

Alharbi

2023

Pharmaceutics

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“…Parthasarathy et al reported a statistically significant increase in the percentage of poor plaque scores among individuals aged over 50 compared to those in the 35 to 50 years age group 32. While in vitro studies have shown that HA significantly impedes the growth and formation of biofilms by Pg,67,68 clinical investigations have not yet established the effectiveness of HA in reducing bacteria. In a systematic review conducted byAlshehri and Alharbi,…”

mentioning

confidence: 99%

Effectiveness of 0.2% Hyaluronic Acid on Clinical, Biomolecular and Microbiological Parameters in Type 2 Diabetes Mellitus Patients with Periodontitis

Devina,

Halim,

Meivi

et al. 2024

Eur J Dent

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Objective This double-blind randomized clinical trial assessed the effectiveness of 0.2% hyaluronic acid (HA) gel as an adjunct to scaling and root planning (SRP) in patients with periodontitis and type 2 diabetes mellitus (DM), focusing on changes in clinical periodontal parameters, the expression of inflammatory mediators, and oral pathogens. Materials and Methods The randomized clinical trial involved 36 participants, 18 DM patients, and 18 healthy patients. The participants in each group were randomly assigned to receive placebo or HA gel after SRP. Gingival crevicular fluid and subgingival plaque samples were taken before treatment and at 4-week follow-up. Clinical parameters, interleukin-1β (IL-1β) and IL-10 levels, and proportions of Porphyromonas gingivalis (Pg) and Fusobacterium nucleatum (Fn) were evaluated at baseline and follow-up. Statistical Analysis Paired t-test (parametric data) or Wilcoxon signed-rank test (nonparametric data) was used for intragroup comparison between baseline and follow-up, and comparisons between groups one-way analysis of variance test (parametric data) or Kruskal–Wallis test (nonparametric data). Results At 4 weeks, most of the groups showed statistically significant decreases (p ≤ 0.05) in various clinical and biomolecular parameters. However, there were exceptions: the pocket probing depth (PPD) and clinical attachment loss (CAL) parameter did not significantly decrease for the placebo (p > 0.05) non-DM group, and the IL-10 parameter in the DM HA gel group (p = 0.108). Regarding bacterial proportions, the non-DM and DM placebo group exhibited significant test results for Pg after 4 weeks (p ≤ 0.05). In the case of Fn bacteria proportions, they decreased in all groups, but these results were not statistically significant (p ≥ 0.05). An intergroup analysis revealed no significant differences (p ≤ 0.05) for bleeding on probing (BOP), PPD, and both proinflammatory and anti-inflammatory cytokines. Only clinical attachment loss (CAL) exhibited a statistically significant intergroup difference 0.042. Conclusion The use of 0.2% HA gel into periodontal pockets alongside SRP, for both diabetic and healthy individuals, showed no statistically significant variances in clinical, biomolecular, and microbiological measures.

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Dental biofilm: control of formation, disorganization and means of regulation

Zabolotna

2023

MTT

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In press Biofilm is a predominant growth form of a greater number of microorganisms in the oral cavity that has the differences in physiology and genetics compared to planktonic forms. It requires a review of methods of the diagnostics and management of patients with the diseases caused by dental biofilms. The literature review was conducted with the aim of summarizing modern scientific information related to the control of its formation and evaluation of means for disorganization and regulation that are used in clinical dentistry. Based on the results of these studies a close relationship between the level of hygiene and the microbiological state of the oral cavity has been justified. Carrying out individual and professional hygiene is an important component in the comprehensive treatment of dental patients. It has been proven that the pathogenicity of the dental biofilm can be significantly reduced by breaking the integrity of its structure and restoring the normal microflora with the help of high-quality professional hygiene. But long-term uncontrolled use of personal hygiene products can cause some changes in the quality of the microbiome of the oral cavity. Modern research is aimed at disorganization of the biofilm matrix by pharmaceutical and non-pharmacological means. But its destruction can lead to the release of living microorganisms and the dissemination of the pathogen. The presented results of the use of different groups of means for normalizing the microflora of the oral cavity have both advantages and disadvantages that requires further discussion. Qualitative control over the development of dental biofilm will permit to anticipate the course of teeth diseases and periodontal tissues, and objectively assess the effectiveness of the prescribed treatment. Conducting a microbiological study of the biocenosis of the oral cavity will be the basis for choosing personal hygiene products and implementing timely measures. All this will further contribute to increase in the efficiency of prevention and management of pathology of the teeth. Keywords: microorganisms, оral cavity, biofilm, hygiene.

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High molecular weight hyaluronic acid reduces the growth and biofilm formation of the oral pathogen Porphyromonas gingivalis

Cited by 3 publications

References 21 publications

The Effect of Adjunctive Use of Hyaluronic Acid on Prevalence of Porphyromonas gingivalis in Subgingival Biofilm in Patients with Chronic Periodontitis: A Systematic Review

The Effect of Adjunctive Use of Hyaluronic Acid on Prevalence of Porphyromonas gingivalis in Subgingival Biofilm in Patients with Chronic Periodontitis: A Systematic Review

Effectiveness of 0.2% Hyaluronic Acid on Clinical, Biomolecular and Microbiological Parameters in Type 2 Diabetes Mellitus Patients with Periodontitis

Dental biofilm: control of formation, disorganization and means of regulation

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