High Levels of Resistance to Cephalosporins Associated with the Presence of Extended-Spectrum and AmpC β-Lactamases in <i>Escherichia coli</i> from Broilers in Southern Brazil

Lentz, Silvia Adriana Mayer; Adam, Franciele Caroline; Rivas, Paula Marques; Souza, Sara Neves de; Cupertino, Victória Martins Lima; Boff, Roberta; Motta, Amanda de Souza da; Wink, Priscila Lamb; Barth, Afonso Luís; Martins, Andreza Francisco

doi:10.1089/mdr.2019.0050

Cited by 5 publications

(3 citation statements)

References 24 publications

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“…For example, in a study of our group, 38.5% of Staphylococcus spp. isolates obtained from horses were resistant to at least one antimicrobial (Mota et al, 2021) study in broilers from Rio Grande do Sul also shows high levels of extended-spectrum beta-lactamases' resistance in E. coli isolates (Lentz et al, 2020). Studies evaluating Enterococcus spp.…”

Section: Discussionmentioning

confidence: 99%

Multidrug‐resistant Escherichia coli from free‐living pigeons (Columba livia): Insights into antibiotic environmental contamination and detection of resistance genes

Bueno

Loiko

Vidaletti

et al. 2022

Zoonoses and Public Health

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Bacterial resistance is a public and one health problem. Free‐living birds can be reservoirs of multidrug‐resistant bacteria and resistance genes. This study aimed to characterize the antimicrobial resistance of Escherichia coli isolated from free‐living urban pigeons (Columba livia) in South Brazil. Ninety‐two animals were sampled, and one isolate was obtained from each one. The isolates were characterized, and the antimicrobial resistance profile and beta‐lactam and colistin resistance genes were investigated. The isolates were classified as phylogroups B1 (35%), B2 (33%), A (16%) and D (16%), and 14% of the strains had the eae virulence gene. All isolates were resistant to at least one antimicrobial, and 63% of them were multidrug‐resistant. Geographical location where the pigeons were captured and presence of the eae gene were associated with multidrug resistance. blaVIM and mcr‐1 genes were detected in one and two isolates, respectively. This is the first report of these genes in E. coli of pigeons. The blaVIM‐positive isolate was classified as Shiga toxin‐producing E. coli, and the isolates with mcr‐1 were classified as Enterohaemorrhagic E. coli and Enteropathogenic E. coli, which raise additional concerns related to public health since these are zoonotic pathotypes. The results reveal that pigeons carry multidrug‐resistant pathogenic E. coli, which may interest public health. Nonetheless, further studies on whether these animals are sources of contamination for humans must be performed to understand their role in spreading antimicrobial resistance.

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Section: Discussionmentioning

confidence: 99%

Multidrug‐resistant Escherichia coli from free‐living pigeons (Columba livia): Insights into antibiotic environmental contamination and detection of resistance genes

Bueno

Loiko

Vidaletti

et al. 2022

Zoonoses and Public Health

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“…Resistant bacteria can then be transferred through several routes to different environments, humans, and animals 26 . Increased use of antibiotics on the farm is reported to drive the evolution of antimicrobial resistance in animals and bacteria confer similar mechanisms of resistance to the commonly used antibiotics in humans 34,36 . Control of antimicrobial resistance, therefore, requires joint efforts from all stakeholders from human health, animal health, agriculture, aquaculture, finance, environment, and well-informed consumers.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance at the human–animal interface in the Pastoralist Communities of Kasese District, South Western Uganda

Iramiot

Kajumbula

Bazira

et al. 2020

Sci Rep

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Intensive usage of antimicrobials in the management of animal diseases leads to selection for resistance among microorganisms. This study aimed to assess antimicrobial use and to describe factors associated with the transmission of antimicrobial resistance between humans and animals in pastoralist communities of Kasese district. A mixed-methods approach was employed in this study. Rectal swabs were collected from the participants and cattle and transported in Carry–Blaire transport medium to the laboratory within 24 h of collection for culture and sensitivity to confirm carriage of multi-drug resistant bacteria. In-depth interviews were conducted among veterinary officers, veterinary drug vendors, human health facility in-charges in both public and private health facilities, and operators of human pharmacies and drug shops. Carriage of multi-drug resistant bacteria among humans was 88 (93%) and 76(80%) among cattle. Consumption of lakeshore water and carriage of multi-drug resistant bacteria in cattle were associated with carriage of multi-drug resistant bacteria in the human population. The prevalence of multi-drug resistance among organisms Isolated from both humans and animals was high. There is a high likelihood of transmission of multi-drug resistance between humans and animals.

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“…In Brazil, MDR E. coli (MDR-E) has already been described in farm animals [23,24], chicken carcasses [25], wild birds [26], coastal waters [27], and as a cause of infections [28], but not as colonizers among individuals in the community. This information is a useful parameter for empiric antimicrobial therapy and a proxy for the general quality of people's health.…”

Section: Introductionmentioning

confidence: 99%

High levels of gut carriage of antimicrobial-resistant Escherichia coli in community settings in Rio de Janeiro, Brazil

et al. 2022

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The prevalence and risk factors for gut carriage of antimicrobial-resistant Escherichia coli among individuals living in the community in Rio de Janeiro, Brazil, are unknown. The aim of this study was to determine the prevalence of colonization with antimicrobial-resistant E. coli, including isolates producing ESBL and harboring plasmid-mediated quinolone resistant (PMQR) genes in this community. We performed a cross-sectional study and analyzed fecal specimens of individuals attending outpatient clinics in the city from January 2015 to July 2019. We investigated susceptibility to antimicrobial agents by disc diffusion tests and used PCR to determine ESBL types, PMQR, and the virulence genes that characterize an isolate as extraintestinal pathogenic E. coli (ExPEC). Among the 623 subjects, 212 (34%) carried an isolate resistant to at least one of the tested antimicrobial agents, with the highest frequencies of resistance to ampicillin (26%), trimethoprim-sulfamethoxazole (19%), cefazolin (14%), and ciprofloxacin (CIP, 9%). In addition, 13% (81) of subjects carried a multidrug-resistant-E. coli (MDR-E), including 47 (8% of all isolates) ESBL-producing E. coli (ESBL-E), mainly of CTX-M-8 (15, 32%) and CTX-M-15 (9, 20%) types. PMQR genes were present in 7% (42) of all isolates, including 60% (32) of the 53 resistant to CIP. Previous use of antimicrobial agents, particularly fluoroquinolones, was a risk factor for colonization with MDR-E (25%, 20/81 vs 13%, 70/542, p = 0.01), ESBL-E (28%, 13/47, vs 13%, 77/576, p = 0.01), and resistance to CIP (26%, 14/53, vs 12%, 70/570, p = 0.01). The most pathogenic phylogroups B2, C, and D were 37% of the MDR-E, 30% of the ESBL-E, 38% of the CIP-resistant, and 31% of PMQR gene carrying E. coli isolates. We show that carriage of MDR-E (mostly ESBL-E) reached high levels in the community in Rio de Janeiro, increased by the selection of antimicrobial agents. Much of the resistant E. coli isolates are potential pathogenic strains. The widespread use of antimicrobial agents during the COVID-19 pandemic in Brazil may have worsened this picture.

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High Levels of Resistance to Cephalosporins Associated with the Presence of Extended-Spectrum and AmpC β-Lactamases in Escherichia coli from Broilers in Southern Brazil

Cited by 5 publications

References 24 publications

Multidrug‐resistant Escherichia coli from free‐living pigeons (Columba livia): Insights into antibiotic environmental contamination and detection of resistance genes

Multidrug‐resistant Escherichia coli from free‐living pigeons (Columba livia): Insights into antibiotic environmental contamination and detection of resistance genes

Antimicrobial resistance at the human–animal interface in the Pastoralist Communities of Kasese District, South Western Uganda

High levels of gut carriage of antimicrobial-resistant Escherichia coli in community settings in Rio de Janeiro, Brazil

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