High levels of multidrug resistance in clinical isolates of Gram-negative pathogens from Nigeria

Ogbolu, David Olusoga; Daini, OA; Ogunledun, A; Alli, A. O. Terry; Webber, Mark A.

doi:10.1016/j.ijantimicag.2010.08.019

Cited by 103 publications

(98 citation statements)

References 10 publications

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“…This is higher than the 2.7 %, 15.8 %, 20 % and 28.9 % reported in previous studies in Nigeria [8,12,18,19]. Previous use of antimi-crobial agents, especially cephalosporins and fluoroquinolones, has been reported as risk factors associated with emergence of ESBL [6,7].…”

Section: Discussionmentioning

confidence: 59%

“…This will indicate that majority of the isolates producing ESBL in this study produce multiple types of ESBL such as TEM, SHV and CTX-M types. Indeed previous studies have reported both TEM, SHV and CTX-M types of ESBL in Nigeria [8,17]. Five isolates (4.9 %) produced only CTX-M type.…”

Section: Discussionmentioning

confidence: 72%

“…Previous use of antimi-crobial agents, especially cephalosporins and fluoroquinolones, has been reported as risk factors associated with emergence of ESBL [6,7]. Both antimicrobial agents are the drug of choice in treating Gram negative bacterial infections [8]. Unregulated use of antibiotics and over the counter sales of antibiotics without prescription is rife in Nigeria [9][10][11].…”

Section: Discussionmentioning

confidence: 99%

“…Risk factors that have been associated with ESBL production include old age (> 65 years), male gender, previous use of β-lactam antibiotics and fluoroquinolones amongst others [6,7]. In Nigeria, extended-spectrum cephalosporins and fluoroquinolones are widely used as broadspectrum antibiotics and remain the drugs of choice to treat infections caused by various Gram negative pathogens [8]. Also, antibiotics use in Nigeria is unregulated and over the counter sales of antibiotics without prescriptions are rife [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

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Extended-Spectrum Beta-Lactamase (ESBL)–Producing Gram-negative Isolates from Urine and Wound Specimens in a Tertiary Health Facility in Southern Nigeria

Ogefere¹,

Aigbiremwen²,

Omoregie³

2015

Trop. J. Pharm Res

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Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Extended-Spectrum Beta-Lactamase (ESBL)–Producing Gram-negative Isolates from Urine and Wound Specimens in a Tertiary Health Facility in Southern Nigeria

Ogefere¹,

Aigbiremwen²,

Omoregie³

2015

Trop. J. Pharm Res

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“…According to previous reports, the mechanisms underlying the resistance of A. baumannii to β-lactam antibiotics can be described as follows: 1) generation of A-D types of β-lactamase that hydrolyze β-lactam drugs (Bush, 2010;Ogbolu et al, 2011); 2) the outer membrane protein (OMP) variants with molecular weights of 29 and 43 kDa are related to carbapenem resistance (Limansky et al, 2002;Dupont et al, 2005); 3) target sites of β-lactam drugs, or penicillin-binding proteins (PBPs) change, e.g. the resistance of A. baumannii to carbapenems correlates with downregulation of PBPs (Perez et al, 2007;Zarrilli et al, 2009); and 4) it has been reported that overexpression of efflux pumps, such as adeABC, adeIJK, and adeFGH on the inner membrane, is associated with increasing minimal inhibitory concentration (MIC) of some β-lactam drugs (Cortez-Cordova and Kumar, 2011;Coyne et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

A novel variant of the β-lactamase ADC-61 gene in multi-drug resistant Acinetobacter baumannii

Zhou

Yang

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The aim of this study was to investigate the existence of a β-lactamase gene in a group of multi-drug resistant Acinetobacter baumannii. Twenty strains of multi-drug resistant A. baumannii were isolated. Thirty-four β-lactamase genes and the ISaba1-OXA-23 linkage were analyzed in these strains by polymerase chain reaction (PCR) and verified by DNA sequencing. Three kinds of β-lactamase genes (TEM, ADC, and OXA-23) were identified, among which the sequence of strain No. 20, ADC, was different from ADC subtypes recorded by GenBank, and was identified as a new variant of β-lactamase genes (named ADC-61 and registered in GenBank: accession No. JQ753702); all the other 19 strains were ADC-30. Eighteen strains of the OXA-23 group were all positive as indicated by detection of ISaba1-OXA-23 linkage. Gene sequencing indicated that the TEM gene was TEM-1. These results suggest that the three kinds of β-lactamase genes identified in this study, TEM, ADC, and OXA-23, play a key role in drug resistance in this group of A. baumannii. To our knowledge, this is the first report of an emergent new mutation of the β-lactamase gene, ADC-61, in China or abroad.

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Coastal Seawater Bacteria Harbor a Large Reservoir of Plasmid-Mediated Quinolone Resistance Determinants in Jiaozhou Bay, China

Zhao

Dang

2012

Microb Ecol

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Diversity and prevalence of plasmid-mediated quinolone resistance determinants were investigated in environmental bacteria isolated from surface seawater of Jiaozhou Bay, China. Five qnr gene alleles were identified in 34 isolates by PCR amplification, including qnrA3 gene in a Shewanella algae isolate, qnrB9 gene in a Citrobacter freundii isolate, qnrD gene in 22 Proteus vulgaris isolates, qnrS1 gene in 1 Enterobacter sp. and 4 Klebsiella spp. isolates, and qnrS2 gene in 1 Pseudomonas sp. and 4 Pseudoalteromonas sp. isolates. The qnrC, aac(6')-Ib-cr, and qepA genes could not be detected in this study. The 22 qnrD-positive Proteus vulgaris isolates could be differentiated into four genotypes based on ERIC-PCR assay. The qnrS1 and qnrD genes could be transferred to Escherichia coli J53 Azi(R) or E. coli TOP10 recipient strains using conjugation or transformation methods. Among the 34 qnr-positive isolates, 30 had a single point mutation in the QRDRs of GyrA protein (Ala67Ser, Ser83Ile, or Ser83Thr), indicating that cooperation of chromosome- and plasmid-mediated resistance contributed to the spread and evolution of quinolone resistance in this coastal bay. Eighty-five percent of the isolates were also found to be resistant to ampicillin, and bla(CMY), bla(OXY), bla(SHV), and bla(TEM) genes were detected in five isolates that also harbored the qnrB9 or qnrS1 gene. Our current study is the first identification of qnrS2 gene in Pseudoalteromonas and Pseudomonas strains, and qnrD gene in Proteus vulgaris strains. High prevalence of diverse qnr genes in Jiaozhou Bay indicates that coastal seawater may serve as an important reservoir, natural source, and dissemination vehicle of quinolone resistance determinants.

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High levels of multidrug resistance in clinical isolates of Gram-negative pathogens from Nigeria

Cited by 103 publications

References 10 publications

Extended-Spectrum Beta-Lactamase (ESBL)–Producing Gram-negative Isolates from Urine and Wound Specimens in a Tertiary Health Facility in Southern Nigeria

Extended-Spectrum Beta-Lactamase (ESBL)–Producing Gram-negative Isolates from Urine and Wound Specimens in a Tertiary Health Facility in Southern Nigeria

A novel variant of the β-lactamase ADC-61 gene in multi-drug resistant Acinetobacter baumannii

Coastal Seawater Bacteria Harbor a Large Reservoir of Plasmid-Mediated Quinolone Resistance Determinants in Jiaozhou Bay, China

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