High-Level Production of Human Parathyroid Hormone (hPTH) by Induced Expression inSaccharomyces cerevisiae

Vad, Randi; Moe, Elin; Saga, Kirsti; Kvinnsland, Aina M.V.; Øyen, Tordis B.

doi:10.1006/prep.1998.0912

Cited by 9 publications

(4 citation statements)

References 31 publications

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“…This is in agreement with a study on enhancement of production of heterologous ␣-amylase by S. cerevisiae (21). In contrast, Vad et al found that even if overexpression of Sso2p in S. cerevisiae enhanced secretion, the total amount of the heterologous protein, human parathyroid hormone, was not increased due to impaired growth of the cells (29).…”

Section: Discussionsupporting

confidence: 91%

Development of a Saccharomyces cerevisiae Strain with Enhanced Resistance to Phenolic Fermentation Inhibitors in Lignocellulose Hydrolysates by Heterologous Expression of Laccase

Larsson

Cassland

Jönsson

2001

Appl Environ Microbiol

280

117

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To improve production of fuel ethanol from renewable raw materials, laccase from the white rot fungus Trametes versicolor was expressed under control of the PGK1 promoter in Saccharomyces cerevisiae to increase its resistance to phenolic inhibitors in lignocellulose hydrolysates. It was found that the laccase activity could be enhanced twofold by simultaneous overexpression of the homologous t-SNARE Sso2p. The factors affecting the level of active laccase obtained, besides the cultivation temperature, included pH and aeration. Laccaseexpressing and Sso2p-overexpressing S. cerevisiae was cultivated in the presence of coniferyl aldehyde to examine resistance to lignocellulose-derived phenolic fermentation inhibitors. The laccase-producing transformant had the ability to convert coniferyl aldehyde at a faster rate than a control transformant not expressing laccase, which enabled faster growth and ethanol formation. The laccase-producing transformant was also able to ferment a dilute acid spruce hydrolysate at a faster rate than the control transformant. A decrease in the content of low-molecular-mass aromatic compounds, accompanied by an increase in the content of high-molecular-mass compounds, was observed during fermentation with the laccase-expressing strain, illustrating that laccase was active even at the very low levels of oxygen supplied. Our results demonstrate the importance of phenolic compounds as fermentation inhibitors and the advantage of using laccase-expressing yeast strains for producing ethanol from lignocellulose.

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Section: Discussionsupporting

confidence: 91%

Development of a Saccharomyces cerevisiae Strain with Enhanced Resistance to Phenolic Fermentation Inhibitors in Lignocellulose Hydrolysates by Heterologous Expression of Laccase

Larsson

Cassland

Jönsson

2001

Appl Environ Microbiol

280

117

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Section: Discussionmentioning

confidence: 99%

Impact of media components from different suppliers on enterokinase productivity in Pichia pastoris

Krahulec

Šafránek

2021

BMC Biotechnol

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Background The aim of this study was to provide an information about the homogeneity on the level of enterokinase productivity in P. pastoris depending on different suppliers of the media components. Results In previous studies, we performed the optimisation process for the production of enterokinase by improving the fermentation process. Enterokinase is the ideal enzyme for removing fusion partners from target recombinant proteins. In this study, we focused our optimization efforts on the sources of cultivation media components. YPD media components were chosen as variables for these experiments. Several suppliers for particular components were combined and the optimisation procedure was performed in 24-well plates. Peptone had the highest impact on enterokinase production, where the difference between the best and worst results was threefold. The least effect on the production level was recorded for yeast extract with a 1.5 fold difference. The worst combination of media components had a activity of only 0.15 U/ml and the best combination had the activity of 0.88 U/ml, i.e., a 5.87 fold difference. A substantially higher impact on the production level of enterokinase was observed during fermentation in two selected media combinations, where the difference was almost 21-fold. Conclusions Results demonstrated in the present study show that the media components from different suppliers have high impact on enterokinase productivity and also provide the hypothesis that the optimization process should be multidimensional and for achieving best results it is important to perform massive process also in terms of the particular media component supplier .

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“…The presence of nitrogen components in cultivation media may also be important to protect heterologous proteins in the extracellular medium from proteolysis. Extracellular proteolysis of heterologous proteins is affected by nutritional conditions, and may increase due to glucose exhaustion or carbon starvation [ 45 , 102 , 103 ]. The addition of complex nitrogen sources, such as casamino acids, peptides, amino acids, skim milk or bovine serum albumin has been shown to decrease the degradation of the heterologous proteins by S. cerevisiae and P. pastoris , probably by providing large amounts of protein substrate or reducing the production of extracellular proteases [ 81 , 104 - 110 ].…”

Section: Introductionmentioning

confidence: 99%

Role of cultivation media in the development of yeast strains for large scale industrial use

et al. 2005

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The composition of cultivation media in relation to strain development for industrial application is reviewed. Heterologous protein production and pentose utilization by Saccharomyces cerevisiae are used to illustrate the influence of media composition at different stages of strain construction and strain development. The effects of complex, defined and industrial media are compared. Auxotrophic strains and strain stability are discussed. Media for heterologous protein production and for bulk bio-commodity production are summarized.

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High-Level Production of Human Parathyroid Hormone (hPTH) by Induced Expression inSaccharomyces cerevisiae

Cited by 9 publications

References 31 publications

Development of a Saccharomyces cerevisiae Strain with Enhanced Resistance to Phenolic Fermentation Inhibitors in Lignocellulose Hydrolysates by Heterologous Expression of Laccase

Development of a Saccharomyces cerevisiae Strain with Enhanced Resistance to Phenolic Fermentation Inhibitors in Lignocellulose Hydrolysates by Heterologous Expression of Laccase

Impact of media components from different suppliers on enterokinase productivity in Pichia pastoris

Role of cultivation media in the development of yeast strains for large scale industrial use

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