High level of endogenous l-serine initiates senescence in Spirodela polyrrhiza

Zhu, Yerong; Tao, Hanlin; Lv, Xin; Wang, Shufang; Wang, Ningning; Wang, Yong

doi:10.1016/j.plantsci.2003.12.021

Cited by 23 publications

(12 citation statements)

References 34 publications

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“…Daughter fronds will separate from their parents after maturation and new daughters will be produced. Thus within a short period a large population can be produced from a single mother frond through vegetative budding 23, 24 . Spirodela polyrrhiza is largely made up of metabolically active cells with very little structural fibre, and the tissue contains twice the protein, fat, nitrogen and phosphorus of other vascular plants.…”

Section: Resultsmentioning

confidence: 99%

Synthesis and herbicidal activity of novel α,α,α‐trifluoro‐m‐tolyl pyridazinone derivatives

Zou

Zhu

et al. 2006

Pest Management Science

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A series of novel alpha,alpha,alpha-trifluoro-m-tolyl pyridazinone derivatives was synthesised. Herbicidal activities of the two intermediate compounds and 15 pyridazinone derivatives were evaluated through barnyardgrass and rape cup tests and Spirodela polyrrhiza (L.) Schleiden tests. Selected compounds were also evaluated under greenhouse conditions. Bleaching activities were observed at 10 microg ml(-1) and some compounds exhibited herbicidal activities at a rate of 300 g ha(-1). The relationship between crystal structures and herbicidal activities is discussed through a comparison of two compounds (5a and 5f).

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Section: Resultsmentioning

confidence: 99%

Synthesis and herbicidal activity of novel α,α,α‐trifluoro‐m‐tolyl pyridazinone derivatives

Zou

Zhu

et al. 2006

Pest Management Science

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“…Kematian kalus hasil kultur anter Anthurium pada penelitian ini diduga terjadi karena dampak senesensi akibat perlakuan serin seperti yang juga dilaporkan oleh Zhu et al (2004) pada kultur in vitro Spirodela polyrrhiza, bahwa aplikasi serin menyebabkan terjadinya senesensi eksplan baik sebagian maupun seluruhnya, baik pada inkubasi terang maupun gelap yang berakibat pada terjadinya kematian. Aplikasi 1 mM menyebabkan terjadinya peningkatan konsentrasi serin andogenus eksplan.…”

Section: Pengaruh Glutamin Dan Serin Dalam Induksi Kalusunclassified

Pengaruh Glutamin dan Serin terhadap Kultur Anter Anthurium andraeanum cv. Tropical

Winarto¹

2011

J.Hort

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Kultur anter merupakan salah satu teknologi haploid penting dalam produksi tanaman haploid ganda dan berhasil diaplikasikan pada berbagai jenis tanaman, namun aplikasi pada Anthurium belum pernah dilaporkan. Penelitian dan pengembangan kultur anter Anthurium yang difokuskan untuk mempelajari pengaruh glutamin dan serin terhadap induksi, pertumbuhan, dan regenerasi kalus dilakukan di Laboratorium Kultur Jaringan Balai Penelitian Tanaman Hias dari bulan Januari sampai dengan September 2008. Tujuan penelitian ialah mengetahui pengaruh kombinasi konsentrasi glutamin dan serin terhadap induksi, pertumbuhan, dan regenerasi kalus pada kultur anter Anthurium. Spadik Anthurium andraeanum cv. Tropical, kalus hasil kultur anter serta medium Winarto dan Teixeira digunakan dalam studi ini. Glutamin dan serin pada konsentrasi 0, 250, 500, dan 750 mg/l diuji dalam percobaan ini. Percobaan disusun menggunakan rancangan acak lengkap pola faktorial dengan empat ulangan. Hasil studi menunjukkan bahwa penambahan glutamin dan serin pada medium terseleksi belum memberikan pengaruh yang signifikan terhadap induksi, pertumbuhan, dan regenerasi kalus. Glutamin pada konsentrasi 250 mg/l menginduksi potensi tumbuh anter hingga 48% dengan 21% anter beregenerasi dan 1,3 anter per perlakuan membentuk kalus. Sementara serin pada 500 mg/l merupakan konsentrasi yang paling potensial dalam induksi kalus dengan 55% potensi tumbuh anter, 24% anter beregenerasi, dan 1,4 anter per perlakuan membentuk kalus. Glutamin 250 mg/l merupakan konsentrasi terbaik dibanding konsentrasi yang lain dalam mendukung pertumbuhan dan regenerasi kalus. Perlakuan tersebut tanpa serin mampu menginduksi potensi pertumbuhan kalus hingga 77% dengan volume kalus mencapai 237 mm3 dan empat tunas dihasilkan per eksplan. Sementara perlakuan serin justru mereduksi pertumbuhan dan regenerasi kalus dan menstimulasi senesensi kalus yang berdampak pada pencoklatan dan kematiannya. Dari hasil penelitian ini dapat disarankan penggunaan glutamin dibanding serin dalam meningkatkan keberhasilan kultur anter Anthurium. Anther culture is one of important haploid technologies in producing double haploid lines and successfully applied in many plants, while the application in Anthurium is not reported yet. Research and development in anther culture of Anthurium focusing on studying the effect of glutamine and serine on callus induction, growth, and its regeneration was conducted at Tissue Culture Laboratory of Indonesian Ornamental Crops Research Institute from January untill September 2008. Objective of this study was to know the effect of glutamine and serine on callus induction, growth, and its regeneration in anther culture of Anthurium. Spadix of Anthurium andraeanum cv. Tropical, callus derived from anther and Winarto and Teixeira medium were utilized in the study. Glutamine and serine of 0, 250, 500, and 750 mg/l were tested in the experiments. Factorial experiment was arranged by completely randomized design with four replications. Results of the study indicate that addition of glutamine and serine in selected culture medium gave moderate significant effect on induction, growth, and regeneration of callus. Glutamine in 250 mg/l induced potential growth of anther up to 48% with 21% regenerated anthers and 1.3 anthers per treatment producing calli, while 500 mg/l of serine was better concentration in callus formation with 55% potential growth of callus, 24% regenerated anthers and 1.4 anthers per treatment producing calli. In growth and regeneration of callus, supplementation of serine reduced callus capacity in growth and production of shoots and stimulated callus senescence causing browning and death of it, while 250 mg/l glutamine exhibited positive effect on them. The treatment without serine was able to induce potential growth of callus up to 77% with 237 mm3 per callus and four shoots produced per explants. Results of the study suggest application of glutamine rather than serine in improving anther culture of Anthurium.

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“…However, increased susceptibility to photodamage has been reported in excised rye leaves (Kar et al 1993) during low-light-induced senescence. In duckweed, it was previously found that light in low intensity could induce senescence of Spirodela polyrrhiza explants cultivated on a medium without supplementation of exogenous cytokinins and that application of cytokinin into the medium could endow the excised halffronds a life time similar to intact plants (Li et al 2003;Zhu et al 2004). Therefore, an apparent antagonism between cytokinin and light in the low-light-induced excised plant senescence system was demonstrated.…”

Section: Introductionmentioning

confidence: 99%

Damage of PS II during senescence of Spirodela polyrrhiza explants under long-day conditions and its prevention by 6-benzyladenine

et al. 2006

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The chlorophyll a (Chl a) fluorescence technique was applied to investigate damage of PS II during senescence of excised half-fronds in Spirodela polyrrhiza P143. The green explants showed a typical Chl a fluorescence transient, OJIP. After cultivation of explants under long-day conditions for 8 days, all the J, I, and P steps disappeared, but a clear K band, an indication of senescence, was observed. JIP-test showed that at this time point, the photosynthetic performance index (PI) dropped to zero and the active reaction center (RC) per leaf cross-section (RC/CS) declined to 18%. As the oxygen-evolving complex (OEC) and the chlorophyll content all remained above 42%, it is proposed that the decline in RC contributes more to the appearance of the K band. Supplementation of 6-benzyladenine (6-BA) into the medium at the beginning of cultivation caused dramatic increase in PI, OEC, RC/CS, and chlorophyll content, and at any time before the 8th day reversed the senescence process of the explants. When 6-BA was added after 8 days of cultivation, the PI did not increase anymore, RC/CS and OEC were maintained at 22% and above 40%, respectively, and chlorophyll content decreased continuously further. These data support a view that the decline in RC is crucial for initiation of the irreversible senescence phase of explants cultivated under long-day conditions.

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High level of endogenous l-serine initiates senescence in Spirodela polyrrhiza

Cited by 23 publications

References 34 publications

Synthesis and herbicidal activity of novel α,α,α‐trifluoro‐m‐tolyl pyridazinone derivatives

Synthesis and herbicidal activity of novel α,α,α‐trifluoro‐m‐tolyl pyridazinone derivatives

Pengaruh Glutamin dan Serin terhadap Kultur Anter Anthurium andraeanum cv. Tropical

Damage of PS II during senescence of Spirodela polyrrhiza explants under long-day conditions and its prevention by 6-benzyladenine

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