High Level of APOA1 in Blood and Maternal Fetal Interface Is Associated With Early Miscarriage

Abstract

Early miscarriage (EM) is one of the most devastating obstetrical complications globally affecting the quality of women's life. In the present study, we aimed to identify proteins that correlate with and could act as biomarkers for EM. We performed 2-dimensional gel electrophoresis in chorionic villi samples followed by mass spectrometry for identification of differential protein expression with EM. Proteomic studies detected a total 124 protein spots, out of which 83 spots were differentially expressed between EM and controls in chorionic villi samples. Matrix assisted laser desorbtion/ionization-time of flight (MALDI-TOF) mass spectrometry analysis revealed Apolipoprotein A1 (APOA1) to be the most upregulated protein in the EM group that was validated by Western blotting and Enzyme-linked immunosorbent assay (ELISA) . We found low but not statistically significant level of APOA1 on 21st day of menstruation in comparison to the 7th day. APOA1 level was observed to be the lowest in the first trimester. Hence, this study suggests that low APOA1 expression is critical in establishing pregnancy and elevated APOA1 expression in chorionic villi correlates with EM. Similar observation in serum samples suggests its potential as a marker for the risk of EM.

A Genome-Wide Association Study To Identify Candidate Genes for Metabolic Disorders in Offspring by In Vitro Fertilization

“…APOA1 is related to embryo quality, development, and survival. Studies have indicated that APOA1 expression is significantly increased in chorionic villi, decidua, and serum of patients with IVF and early abortion, and Verma' results also proved that APOA1 expression in the villi of IVF patients was increased [ 53 ] . Furthermore, APOE plays a major role in antiphospholipid functional attenuation (APL), inducing pregnancy complications [54] .…”

Article A Genome-Wide Association Study To Identify Candidate Genes for Metabolic Disorders in Offspring by In Vitro Fertilization

“…APOA1 is related to embryo quality, development, and survival. Studies have indicated that APOA1 expression is signi cantly increased in chorionic villi, decidua, and serum of patients with IVF and early abortion, and Verma' results also proved that APOA1 expression in the villi of IVF patients was increased [53] . Furthermore, APOE plays a major role in antiphospholipid functional attenuation (APL), inducing pregnancy complications [54] .…”

Cardiometabolic health during early adulthood and risk of miscarriage: a prospective study

“…Another study reported altered serum lipids profiles among women with a threatened miscarriage, including diminished concentrations of LDL cholesterol, total cholesterol, and phospoholipids 24 . There are also a few previous studies which found that greater Apolipoprotein A-I might is associated with an increased likelihood of miscarriage 25,26 .…”

“…It is clear that the metabolism of phospholipids changes during pregnancy 30,31 , but we do not fully understand if and what impact this may have on ensuring a healthy pregnancy. With regard to some of the other specific metabolites measured, Apolipoprotein A-I is an immunomodulator, and is shown to inhibit myeloid derived suppressor cell recruitment, which is known to regulate maternal pregnancy tolerance ensuring an intact pregnancy 25,26 .…”