In vitro fertilization (IVF) processes increase offspring's short-term and long-term health risks, but their mechanisms remain unclear. We conducted a bibliometric analysis to determine the landscape of IVF offspring health. Subsequently, a bioinformatics method was utilized to identify the co-genes properties and biological function mechanisms of IVF and type 2 diabetes mellitus (T2DM). Finally, we predicted compounds against key targets and performed multiple validations of the mechanisms underlying IVF offspring health risks. We identified 15 genes associated with T2DM, and their biological functions are primarily associated with lipid metabolism. We also identified the properties of co-genes, modified characteristics, confirmed a conserved motif, identified 3 SNPs sites, and determined the three core genes, APOA1, APOB, and APOE, which were mainly correlated with metabolic and cardiovascular diseases. In addition, we predicted drugs that may improve metabolic abnormalities in IVF offspring. The impact of aberrant lipid metabolism in offspring after IVF therapy warrants additional investigation, particularly in terms of long-term health consequences and possible mechanisms.
Background In vitro fertilization (IVF) processes increase offspring's short-term and long-term health risks, but their mechanisms remain unclear. Methods We conducted a bibliometric analysis to determine the landscape of IVF offspring health. Subsequently, a bioinformatics method was utilized to identify the co-genes properties and biological function mechanisms of IVF and type 2 diabetes mellitus (T2DM). Finally, we predicted compounds against key targets and performed multiple validations of the mechanisms underlying IVF offspring health risks. Results We identified 15 genes associated with T2DM, and their biological functions are primarily associated with lipid metabolism. We also identified the properties of co-genes, modified characteristics, confirmed a conserved motif, identified 3 SNPs sites, and determined the three core genes, APOA1, APOB, and APOE, which were mainly correlated with metabolic and cardiovascular diseases. In addition, we predicted drugs that may improve metabolic abnormalities in IVF offspring. Conclusion The impact of aberrant lipid metabolism in offspring after IVF therapy warrants additional investigation, particularly in terms of long-term health consequences and possible mechanisms.
Based on our previous animal model of abnormal metabolism, we combined bioinformatics methods to explore the mechanism of early embryo oxidative stress increasing metabolic risk in offspring. Methods: To screen the differentially expressed genes (DEGs) between IVF and naturally conceived offspring by bioinformatics methods, and to identify the co-gene characteristics and biological function mechanisms of DEGs and glucose and lipid metabolism related. Then, we constructed oxidative stress models in vitro and in vivo and performed functional analysis of early embryo differential genes caused by oxidative stress to explore the molecular mechanism of abnormal metabolism risk in IVF offspring. Results: In IVF offspring, we found 15 differentially expressed genes associated with disorders of glucose and lipid metabolism, and their biological functions are primarily associated with lipid metabolism. Zygote oxidative stress is a key factor affecting offspring health, and in different oxidative stress models, the level of ROS was significantly increased. More importantly, the PPAR pathway was altered in both IVF offspring and the early embryo oxidative stress model. In addition, we found decreased expression and nuclear localization of PPARγ in oxidative stress on IVF-derived embryos. Conclusion: The results suggest that PPARγ is involved in the development of embryos under oxidative stress. Oxidative stress can lead to genome-wide reprogramming of embryos and affect The long-term health of offspring through PPARγ.
BackgroundIn vitro fertilization (IVF) processes increase offspring's short-term and long-term health risks, but their mechanisms remain unclear. MethodsWe conducted a bibliometric analysis to determine the landscape of IVF offspring health. Subsequently, a bioinformatics method was utilized to identify the co-genes properties and biological function mechanisms of IVF and type 2 diabetes mellitus (T2DM). Finally, we predicted compounds against key targets and performed multiple validations of the mechanisms underlying IVF offspring health risks. ResultsWe identified 15 genes associated with T2DM, and their biological functions are primarily associated with lipid metabolism. We also identified the properties of co-genes, modified characteristics, identified 3 SNPs sites, and determined the three core genes, APOA1, APOB, and APOE, which were mainly correlated with metabolic and cardiovascular diseases. In addition, we predicted drugs that may improve metabolic abnormalities in IVF offspring. ConclusionsThe impact of aberrant lipid metabolism in offspring after IVF therapy warrants additional investigation, particularly in terms of long-term health consequences and possible mechanisms.
In vitro fertilization (IVF) processes increase offspring's short-term and long-term health risks, but their mechanisms remain unclear. We conducted a bibliometric analysis to determine the landscape of IVF offspring health. Subsequently, a bioinformatics method was utilized to identify the co-genes properties and biological function mechanisms of IVF and type 2 diabetes mellitus (T2DM). Finally, we predicted compounds against key targets and performed multiple validations of the mechanisms underlying IVF offspring health risks. We identified 15 genes associated with T2DM, and their biological functions are primarily associated with lipid metabolism. We also identified the properties of co-genes, modified characteristics, confirmed a conserved motif, identified 3 SNPs sites, and determined the three core genes, APOA1, APOB, and APOE, which were mainly correlated with metabolic and cardiovascular diseases. In addition, we predicted drugs that may improve metabolic abnormalities in IVF offspring. The impact of aberrant lipid metabolism in offspring after IVF therapy warrants additional investigation, particularly in terms of long-term health consequences and possible mechanisms.
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