High iron content of Ankaferd hemostat as a clue for its hemostatic action of red blood cell origin

Akar, Nejat; Ardçoğlu, Yasemin; Öktem, Zekı; Erduran, Nuran; Haznedaroğlu, İbrahim C.

doi:10.1097/mbc.0000000000000223

Cited by 7 publications

(8 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2 Akar and coworkers previously demonstrated the presence of Fe(III) ions inside ABS (ABS iron ion concentration were higher than 2000 ppm in their investigation). 3 We, therefore, investigated possible interrelationships between ABS-derived iron and the antithrombin effects of ABS. The aim of this letter is to report the results of those investigations.…”

mentioning

confidence: 99%

The Antithrombin Effect of Ankaferd Hemostat (ABS) Is Related to the High Iron Content of the Medicine

Okay

Öztürk

Haznedaroğlu

2019

Clin Appl Thromb Hemost

Self Cite

View full text Add to dashboard Cite

mentioning

confidence: 99%

The Antithrombin Effect of Ankaferd Hemostat (ABS) Is Related to the High Iron Content of the Medicine

Okay

Öztürk

Haznedaroğlu

2019

Clin Appl Thromb Hemost

Self Cite

View full text Add to dashboard Cite

“…Human fibrinogen immediately recognizes iron. Thus, iron can play an important role in the ABS related cellular hemostasis located in the junction of RBCfibrinogen interactions (19). The antithrombotic effect of ABS may be associated with the presence of Fe (III) ions.…”

Section: Discussionmentioning

confidence: 99%

Effects of Ankaferd Hemostat on red blood cell aggregation: a hemorheological study

Çiftçiler¹,

Aksu²,

FALKMARKEN³

2019

Turk J Med Sci

View full text Add to dashboard Cite

Background/aim: Ankaferd hemostat (ABS; Ankaferd blood stopper, İstanbul, Turkey) is a prohemostatic agent affecting erythrocytes. The hemostatic action of ABS depends upon fibrinogen gamma chain, prothrombin, and red blood cells. The aim of this study was to assess the effects of ABS on erythrocyte aggregation via hemorheological analyses. Materials and methods: To measure erythrocyte aggregation, blood samples were obtained from healthy, nonsmoker volunteers who had not taken any medication in the previous 10 days. One mL of blood was placed into the laser-assisted optical rotational cell analyzer (LORCA), into the chamber formed by the gap between two concentric glass cylinders. The solution prepared with ABS and saline was added to blood in incremental amounts of 10 µL, 20 µL, 30 µL, 40 µL, 50 µL, 60 µL, 70 µL, and 100 µL. Erythrocyte aggregation was determined by laser-assisted optical rotational cell analyzer at 37 °C. Results: AMP was found to be 17.7 ± 2.1 au in the blood without ABS, whereas it was lower in the blood with ABS. AMP was 16.0 ± 3.3 in the ABS-added blood group. RBC aggregates did not form faster when cells contacted ABS. The t t½ value was 4.6 ± 2.6 in the ABS-added blood group and 1.9 ± 0.20 in the control group. Aggregation was faster in the control group (P = 0.03). AI, which is a combination of AMP and t½, was lowered in the ABS group (48.7 ± 12.3) compared to the control group (65.8 ± 1.6) (P = 0.02). It was notable that the γIsc max (sec-1) value of the control was higher (200 ± 106) than the ABS-added blood group (141 ± 51.0). Conclusion: ABS has antierythroid aggregation effect. ABS inhibits pathological aggregation of red blood cells. Antithrombotic clinical effects of ABS may be ascribed to the antierythroid aggregan actions of the drug.

show abstract

“…24 Recently, Akar et al indicated that ABS had significant amount of iron and very low concentration of copper and zinc. 7 Thus, we investigated whether ABS-derived iron can ameliorate DFO-induced iron deficiency in vitro. We used human Caco-2 and HepG2 cell lines as models of this study.…”

Section: Discussionmentioning

confidence: 99%

“…14 The recent investigation has shown that ABS has significant amount of iron. 7 This observation let us hypothesize that iron in ABS solution might have ability to reduce IDA. We tested this idea in iron-deficiency-induced Caco-2 and HepG2 cell lines by analyzing expression of iron-regulating genes.…”

Section: Introductionmentioning

confidence: 97%

“…It has been recently reported that ABS contains iron. 7 Iron is one of the important transition metals in human and involves in variety of physiological functions including oxygen carrying, cell division, inflammation, and energy production. 8 Consuming low levels of dietary iron or bleeding lead to iron-deficiency anemia (IDA) in human, whereas high iron in human body causes iron toxicity in tissues (hemochromatosis).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ankaferd Influences mRNA Expression of Iron-Regulated Genes During Iron-Deficiency Anemia

Gulec

Güleç

2017

Clin Appl Thromb Hemost

View full text Add to dashboard Cite

Ankaferd Blood Stopper (ABS) comprises a mixture of plants and stops bleeding via forming a protein network by erythroid aggregation. Bleeding causes reduction of iron levels in body. It has been indicated that ABS contains significant amount of iron. Thus, we investigated the biological activity of ABS-derived iron on iron-regulated genes during iron-deficiency anemia (IDA). IDA We selected Caco-2 and HepG2 cell lines as in vitro models of human intestine and liver, respectively. Iron deficiency anemia was induced by deferoxamine. The cells were treated with ferric ammonium citrate (FAC) and ABS. Messenger RNA levels of ironregulated genes were analyzed by quantitative reverse transcription polymerase chain reaction to elucidate whether iron in ABS behaved similar to inorganic iron (FAC) during IDA. The results showed that ABS-derived iron influenced transcriptions of ironregulated marker genes, including divalent metal transporter (Dmt1), transferrin receptor (TfR), ankyrin repeat domain 37 (Ankrd37), and hepcidin (Hamp) in IDA-induced Caco-2 and HepG2 cells. Our results suggest that when ABS is used to stop tissue bleeding, it might have an ability to reduce levels of IDA.

show abstract

High iron content of Ankaferd hemostat as a clue for its hemostatic action of red blood cell origin

Cited by 7 publications

References 3 publications

The Antithrombin Effect of Ankaferd Hemostat (ABS) Is Related to the High Iron Content of the Medicine

The Antithrombin Effect of Ankaferd Hemostat (ABS) Is Related to the High Iron Content of the Medicine

Effects of Ankaferd Hemostat on red blood cell aggregation: a hemorheological study

Ankaferd Influences mRNA Expression of Iron-Regulated Genes During Iron-Deficiency Anemia

Contact Info

Product

Resources

About