High hydrostatic pressure affects antigenic pool in tumor cells: Implication for dendritic cell-based cancer immunotherapy

Urbanová, Linda; Hradilova, Nada; Moserová, Irena; Vošáhlíková, Šárka; Sadílková, Lenka; Hensler, Michal; Špíšek, Radek; Adkins, Irena

doi:10.1016/j.imlet.2017.05.005

Cited by 18 publications

(27 citation statements)

References 23 publications

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“…HHP treatment of selected human cancer cell lines leads to degradation of tumor antigens. Of note is that this is dependent on both, the tumor entity or even individual tumors and the magnitude of HHP . Our experiments revealed that HHP with 200 MPa and 500 MPa is in particular immunogenic (and C. Seitz et al, unpublished data).…”

Section: Immunological Basis For Design Of Multimodal Radio‐immunothesupporting

confidence: 49%

“…Of note is that this is dependent on both, the tumor entity or even individual tumors and the magnitude of HHP. 163 Our experiments revealed that HHP with 200 MPa and 500 MPa is in particular immunogenic 159 HHP-treatment therefore fulfills, besides staying in accordance with legal requirements and showing no toxicity, the main preconditions for the generation of whole cell-based tumor vaccines. HHPtreated tumor cells are completely inactivated and maintain their immunogenicity, with HHP-treatment being a highly reproducible and relatively easy method.…”

Section: Combination Of Rt With Vaccination-high Hydrostatic Pressumentioning

confidence: 52%

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Immunomodulation by ionizing radiation—impact for design of radio‐immunotherapies and for treatment of inflammatory diseases

Frey

Rückert

Deloch

et al. 2017

Immunological Reviews

149

127

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Ionizing radiation is often regarded as an element of danger. But, danger responses on the cellular and molecular level are often beneficial with regard to the induction of anti-tumor immunity and for amelioration of inflammation. We outline how in dependence of radiation dose and fraction, radiation itself-and especially in combination with immune modulators-impacts on the innate and adaptive immune system. Focus is set on radiation-induced changes of the tumor cell phenotype and the cellular microenvironment including immunogenic cancer cell death. Mechanisms how anti-tumor immune responses are triggered by radiotherapy in combination with hyperthermia, inhibition of apoptosis, the adjuvant AnnexinA5, or vaccination with high hydrostatic pressure-killed autologous tumor cells are discussed. Building on this, feasible multimodal radio-immunotherapy concepts are reviewed including overcoming immune suppression by immune checkpoint inhibitors and by targeting TGF-β. Since radiation-induced tissue damage, inflammation, and anti-tumor immune responses are interconnected, the impact of lower doses of radiation on amelioration of inflammation is outlined. Closely meshed immune monitoring concepts based on the liquid biopsy blood are suggested for prognosis and prediction of cancer and non-cancer inflammatory diseases. Finally, challenges and visions for the design of cancer radio-immunotherapies and for treatment of benign inflammatory diseases are given.

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Section: Immunological Basis For Design Of Multimodal Radio‐immunothesupporting

confidence: 49%

Section: Combination Of Rt With Vaccination-high Hydrostatic Pressumentioning

confidence: 52%

Immunomodulation by ionizing radiation—impact for design of radio‐immunotherapies and for treatment of inflammatory diseases

Frey

Rückert

Deloch

et al. 2017

Immunological Reviews

149

127

View full text Add to dashboard Cite

show abstract

“…Although HHP is known to denature proteins, it does not affect the covalent bonds, meaning the primary and secondary structures of the proteins are maintained, while their tertiary and quaternary structures are changed ( 42 ). A previous study showed that HHP-treated tumor cells affect the antigenic pool and DCs and HHP-killed tumor cells may induce CD8 + T cell-mediated responses ( 43 ). Moreover, HHP-treatment induces immunogenic tumor cell death, and the interaction between tumor cells killed with HHP and DC results in the phagocytosis of cancer cells and activation of DC ( 44 ).…”

Section: Discussionmentioning

confidence: 99%

Effective pressure and treatment duration of high hydrostatic pressure to prepare melanoma vaccines

Yan

et al. 2020

Oncol Lett

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Current therapeutic methods for melanoma have numerous limitations, and thus the improvement of such treatment methods are essential. One possible option is the vaccination of autologous inactivated tumor cells. The primary indispensable principles of a cell-based melanoma vaccine include: i) Entire inactivation of melanoma cells; ii) retaining the immunogenicity of melanoma cells; and iii) adherence to laws and ethical guidelines. However, traditional methods for the production of the vaccine, such as ultrasonic, chemotherapeutics and freeze-thawing, have some juridical or therapeutic constraints. Therefore, the present study used high hydrostatic pressure (HHP) to inactivate malignant cells, and treated B16-F10 tumor cells with different pressures (≥50 MPa) and different durations (≥1 min). It was identified that tumor cells in vitro lost their proliferative ability, but retained their immunogenicity following treatment. Furthermore, the vaccination of the melanoma cells significantly suppressed their oncogenesis. Collectively, the present results suggest that HHP treatment may be an economically viable and effective measure to develop a melanoma vaccine, when pressure was ≥200 MPa and the treatment duration was ≥30 min.

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“…According to Pascal's law, pressure which is generated and transmitted by the transmitting fluid acts to the same amount on the cells filled in the cryovials. The fluid is stored in a reservoir (5) that is attached to the aperture and the pressure can be recorded via a digital manometer (6). The pressure is maintained and released by several switches (7) in the aperture.…”

Section: Methodsmentioning

confidence: 99%

“…Though HHP is known to denature proteins, it doesn't affect covalent bonds, meaning that the proteins' primary and secondary structure is maintained, whereas their tertiary and quaternary structure is changed (5). Urbanova et al showed that HHP-treatment of tumor cells affects the antigenic pool and that loading of DCs with HHP-killed tumor cells can induce CD8+ T cell responses in vitro (6). Fucikova et al demonstrated that HHP-treatment induces immunogenic cancer cell death in human tumor cells and that interaction of HHP-killed cancer cells with DCs results in phagocytosis of the tumor cells and activation of the DCs (7).…”

Section: Introductionmentioning

confidence: 99%

Tumor Cell-Based Vaccine Generated With High Hydrostatic Pressure Synergizes With Radiotherapy by Generating a Favorable Anti-tumor Immune Microenvironment

et al. 2019

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Dendritic cell (DC)-based vaccines pulsed with high hydrostatic pressure (HHP)-inactivated tumor cells have been demonstrated to be a promising immunotherapy for solid tumors. We focused on sole injection of tumor cells that were inactivated by HHP and their combination with local radiotherapy (RTx) for in vivo induction of anti-tumor immune responses. HHP-treatment of tumor cells resulted in pre-dominantly necrotic cells with degraded DNA. We confirmed that treatments at 200 MPa or higher completely inhibited the formation of tumor cell colonies in vitro . No tumor growth was seen in vivo after injection of HHP-treated tumor cells. Single vaccination with HHP-killed tumor cells combined with local RTx significantly retarded tumor growth and improved the survival as shown in B16-F10 and CT26 tumor models. In B16-F10 tumors that were irradiated with 2 × 5Gy and vaccinated once with HHP-killed tumor cells, the amount of natural killer (NK) cells, monocytes/macrophages, CD4+ T cells and NKT cells was significantly increased, while the amount of B cells was significantly decreased. In both models, a trend of increased CD8+ T cell infiltration was observed. Generally, in irradiated tumors high amounts of CD4+ and CD8+ T cells expressing PD-1 were found. We conclude that HHP generates inactivated tumor cells that can be used as a tumor vaccine. Moreover, we show for the first time that tumor cell-based vaccine acts synergistically with RTx to significantly retard tumor growth by generating a favorable anti-tumor immune microenvironment.

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High hydrostatic pressure affects antigenic pool in tumor cells: Implication for dendritic cell-based cancer immunotherapy

Cited by 18 publications

References 23 publications

Immunomodulation by ionizing radiation—impact for design of radio‐immunotherapies and for treatment of inflammatory diseases

Immunomodulation by ionizing radiation—impact for design of radio‐immunotherapies and for treatment of inflammatory diseases

Effective pressure and treatment duration of high hydrostatic pressure to prepare melanoma vaccines

Tumor Cell-Based Vaccine Generated With High Hydrostatic Pressure Synergizes With Radiotherapy by Generating a Favorable Anti-tumor Immune Microenvironment

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